In this study, matured silkworm hemolymph was studied for inhibitory effect on melanocyte formation, tyrosinase activities and antibacterial effect. A matured silkworm hemolymph mass purification method was developed and some of suppressing melanin formation effects were investigated, too. Antibacterial effects were tested by Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and Candida albicans. As a result, the advanced purification method was higher in yield rate than existing one by 53%. A free radical scavenging activity of hemolymph was higher than sericin on the market(IC50, 202.9±33.1) and lower than vitamin C(0.57±0.02) by 6.4±0.3 ㎍/㎖. An inhibitory effect on melanin synthesis was higher in hemolymph compared to morus bark and arbutin by 9.15% and 11.56%, respectively. The antibacterial effects on Escherichia, Staphylococcus were detected at 2% hemolymph concentration, which was relatively good. Thus, these results suggest that hemolymph of matured silkworm may have beneficial properties as a material for cosmetics.