The vertical distributions of nitrifying bacteria in aerobic fixed biofilm were investigated to evaluate the relationship between nitrification performance and microbial community at different HRT. Fluorescent in situ hybridization (FISH) and portable ion selective microelectrode system were adopted to analyze microbial communities and ions profiles according to the biofilm depth. Cilia media packed MLE (Modified Ludzack-Ettinger) like reactor composed of anoxic, aerobic Ⅰ/Ⅱ was operated with synthetic wastewater having COD 200 mg/L and NH4 +-N 40 mg/L at HRT of 6 hrs and 4 hrs. Total biofilm thickness of aerobic Ⅰ, Ⅱ reactor at 4 hrs condition was over two times than that of 6 hrs condition due to the sufficient substrate supply at 4 hrs condition (6 hrs; aerobic Ⅰ 380 ㎛ and Ⅱ 400 ㎛, 4 hrs; aerobic Ⅰ 830 ㎛ and Ⅱ 1040 ㎛). As deepen the biofilm detection point, the ratio of ammonia oxidizing bacteria (AOB) was decreased while the ratio of nitrite oxidizing bacteria (NOB) was maintained similar distribution at both HRT condition. The ratio of AOB was higher at 4 hrs than 6 hrs condition and NH4 +-N removal efficiency was also higher at 4 hrs with 89.2% than 65.4% of 6 hrs. However, the ratio of NOB was decreased when HRT was reduced from 6 hrs to 4 hrs and NO2 --N accumulation was observed at 4 hrs condition. Therefore, it is considered that insufficient HRT condition could supply sufficient substrate and enrichment of AOB in all depth of fixed biofilm but cause decrease of NOB and nitrite accumulation.

This study was performed to evaluate the inactivation and microbial regrowth of heterotrophic and nitrifying bacteria using chloramine as a secondary disinfectant for drinking water distribution system. Three sets of the three reactors filled with the Cl2/NH3-N ratio of 3:1, 4:1 and 5:1 were used in these experiments. Chloramine concentration were applied to each set of the reactors with 1㎎/ℓ, 2㎎/ℓ and 3㎎/ℓ, respectively. For the set with 1㎎/ℓ was applied, all the reactors showed that the residual chloramine concentration gradually decreased with elapsed time and reached to zero level after 7 days. Heterotrophic bacteria remarkably increased and nitrification occurred after 11 days. For the sets with 2㎎/ℓ and 3㎎/ℓ, however, the residual chloramine was maintained through the experimenatal period (21 day). Furthermore the regrowth of heterotrophic bacteria and nitrification were not found. More than 2㎎/ℓ of chloramine with Cl2/NH3-N ratio of 3:1, the nitrification could be inhibited by 2 days of contact time.