표고 시설재배에 있어 적정 상대습도를 구명하여 고품질 버섯생산 데이터를 제시하기 위해 상대습도를 달리 처리한 결과는 다음과 같다. 상대습도에 따른 생육특성을 조사한 결과, 갓의 명도값은 RH65%에서 높았고, 적색도는 RH95%에서 높았으며, 황색도는 상대습도 간 유의적 차이가 나타나지 않았다. 갓의 경도는 RH95%에서 높은 경향이었다. 갓직경은 RH95%에서 높게 측정되었고, 갓두께는 RH95%에서 높았다. 대길이의 경우 유의적 차이가 나타나지 않았지만 상대습도가 증가할수록 대길이가 증가하는 경향을 보였으며, 대직경은 RH95%에서 높게 측정되었다. 수량은 RH95%에서 유의적으로 높게 측정되었다. 화고발생률은 상대습도가 낮아질수록 높게 조사되었다. 위의 결과를 통해 RH95%에서 표고 자실체의 크기, 수량이 높게 조사되었지만 버섯의 갓색이 어둡고 화고 발생 비율이 낮은 결과를 나타내어 시설재배에서 고품질의 화고 버섯을 생산 할 수 있는 상대습도는 65%와 85%인 것으로 판단되었다.

표고 톱밥배지 배양일수별 배지특성을 조사한 결과 ‘산조701호’의 색도는 명도인 L값이 배양 30일에 83.8(±2.5) 에서 배양 120일에 45.7(±2.3)로 감소하였고, a와 b의 값은 배양일수가 증가할수록 증가하였지만 경도는 배양 30일에 9.4(±0.9)g/mm에서 배양 120일에 2.6(±0.2)g/mm으로 감소하였다. ‘농진고’는 명도인 L값이 배양 30일에 86.2(±2.1) 에서 배양 120일에 53.4(±1.3)로 감소하였고, a와 b의 값은 배양일수가 증가할수록 증가하였지만 경도는 배양 30일에 4.8(±0.7)g/mm 배양 120일에 3.8(±1.0)g/mm로 감소하였다. 배양일수별 자실체 특성을 조사한 결과 ‘산조701호’의 배양일수별 첫 수확일은 30일 배양배지는 46일, 60일배양 배지는 89일, 90일배양 배지는 8일로 가장 짧았고, 120일 배양배지가 9일이었다. 자실체 평균무게는 90일 배양배지가 48.3 g으로 가장 높았고 갓 직경, 갓 두께도 가장 높았지만 색도측정결과에서는 120일 배양배지에서 발생된 자실체가 가장 우수한 상품성을 보이는 것을 확인하였다. ‘농진고’는 30일 배양배지가 22일, 60일 배양배지가 18일, 90일 배양 배지가 8일로 가장 짧았고, 120일 배양배지는 9일이었다. 따라서 표고의 배지, 자실체 특성 등을 고려할 때 배지의 배양일수가 120일이 안정적인 수량 및 높은 상품성 확보가 가능할 것으로 판단되었다.

In this study, we investigated the morphological characteristics and antioxidant ability of mushroom cultivar Lentinula edodes ‘Nongjingo’ fruiting bodies after exposure to various light conditions. Color differences between mushrooms treated with mixtures of LED light revealed that mushrooms displayed lighter color shades when compared to the control group (fluorescent light treated mushrooms). Redness increased and yellowness decreased after exposure to all treatments other than the fluorescent control. Measurement of growth characteristics of ‘Nongjingo’ fruiting bodies showed increases after exposure to all mixed LED treatments. In addition, the uniformity of fruiting bodies was higher when using LED light compared to fluorescent light. The measurement of stem diameters did not show a significant difference between the treatments, however, diameters were slightly larger with exposure to white-green LED. Moreover, stem length was longer in the mixed LED treatments when compared to those exposed to fluorescent light. Examination of the ratio of stem diameter to stem length revealed that the diameter of the stem was greater than the length. The antioxidant activity of water extracts made from Nongjingo fruiting bodies grown under mixed LED conditions was compared to those from mushrooms grown under fluorescence light conditions. The highest antioxidant activity was observed from mushrooms treated with white LED; however, no significant difference was found between mushrooms exposed to white-green LED compared to white-blue LED. The treatment showed higher antioxidant ability than vitamin C. Our results confirm that treatment of white LED and white-blue LED affects the growth and antioxidant ability of Nongjingo mushroom fruiting bodies.

At present, oak sawdust and rice bran are mainly used as the medium materials for bag cultivation of Lentinula edodes. This experiment was conducted to develop the optimum medium of ‘Nongjingo’, a new variety of Lentinula edodes. We investigated oak sawdust, oak fermented sawdust, poplar sawdust, and douglas fir sawdust as main materials, and rice bran and wheat bran as nutrient resources, respectively. The medium composition was consisted of main material 80%+nutrient 20% based on the volume ratio. Eight treatments of medium composition were tested. The period of mycelial incubation in ‘oak sawdust 40%+douglas fir sawdust 40%+rice bran 20% was 86 days (39 days in dark incubation and 47 days in browning) shortened up to 18 days compared to the control. The 1st yield of commercial fruiting bodies in this treatment was 48.1 g/bag which was 44.8% higher than that in control. We expect that the cultivation period of ‘Nongjingo’ will be shortened, and the yield increased in the medium replaced half of oak sawdust by douglas fir sawdust.

This research was relative humidity (65, 75, 85 and 95%) growth characteristics of the oak mushroom (Lentinula edodes) 'Nongjingo’. At the relative humidity was 65% in growth chamber. On this condition the moisture content of medium surface was 60.5%. That was lower than other treatments. That condition makes Pileus was 77.1% and stipe was 65%. Among the growth characteristics according to the relative humidity, the pileus diameter at 75% relative humidity were 45.5mm that largest than other treatment and pileus thickness at 75% relative humidity were largest than other treatment. The stipe thickness at 65% relative humidity were 14.8mm that lagest than other treatment. The number of available stipes at 95% relative humidity were 13.3peace/2kg that largest than other treatment. The comparison yield ability of 1 cycle was 104.2g that result in largest than other treatment at 95% relative humidity. This result was associated to the relative humidity of fruiting bodies, the lower relative humidity result in the lower of yield. However, the lower relative humidity result in the higher number of whago, Especially, at the treatment of 65% relative humidity, all the fruit bodies were produced as Whago (dry). Therefore, the higher relative humidity influenced in the higher yield but produced water mushroom. That result was thought that ‘Nongjingo’ would be possible to produce high quality mushroom by adjusting the relative humidity appropriately.