The development of resistant seeds to manage this pest is a big challenge for breeders. We investigated the oviposition preference and development of C. chinensis on two susceptible mungbean cultivars (Seonwha and Gyeongseon) and one previously reported resistant cultivar Jangan, compared to the susceptible cowpea, Yeonbun, by offering a multiple choice test and a no-choice test. Additional, insect stage wise development of C. chinensis was also examined at four temperatures (20, 25, 30 and 35℃). The both choice tests results showed that the cowpea is the most suitable seed host for oviposition. Total developmental time from egg to adult stage ranged from 27.01 to 38.2 days, and developmental time was shortest on the cowpea and longest on Jangan mungbean. However, no successful development of C. chinensis on Jangan mungbean occurred at all constant temperatures. Higher rate of adult emergence and longer adult longevity were found on cowpea and mungbean except Jangan mungbean. Results suggest, higher performance of C. chinensis on cowpea and mungbean might be governed by physical as well as chemical attributes of the seeds. Thus, Jangan mungbean could be stand as a resistant one against bruchids and it could be a resistant sources for successful breeding.

본 연구의 목적은 이른 봄철 계곡에서 번식하는 계곡산개구리 산란지의 수온 및 음환경을 규명하는 것이다. 연구대상 지는 한국 중부지역 치악산국립공원의 75개소이었다. 조사기간은 2015년 3월 23일부터 4월 24일까지이었다. 알덩이수 와 산란지의 물리적 요인간 상관관계 분석결과 산란지 수온과는 양의 상관관계(p<0.05)를, 산란지 음량과는 음의 상관관계(p<0.05)를 나타내었다. 그러나 면적과 수심은 알덩이수와 상관관계를 나타내지 않았다. 계곡산개구리 산란지와 본류 의 수온을 비교한 결과 산란지가 본류보다 평균 2.2℃ 높았다(p<0.001). 계곡산개구리는 이른 봄철 낮은 온도를 극복하 기 위하여 물이 고여 있고, 수심이 10cm 내외로 얕은 곳의 수온이 높은 곳에 산란하고 있었다. 또한 알들을 서로 붙여 낳음으로서 보온효과를 극대화하고 있었다. 계곡산개구리와 산란지의 음량을 본류와 비교한 결과 산란지는 본류보 다 6.9㏈ 조용한 것으로 나타났다(p<0.001). 따라서, 계곡산개구리는 이른 봄철의 낮은 온도, 시끄러운 물소리를 극복하 기 위하여 계곡 내에서도 수온이 높고, 조용한 미소서식지를 찾아 산란하는 것으로 판단된다. 본 논문은 양서류 산란지의 음환경 특성을 밝힌 첫번째 논문이라는데서 의의가 있다.

Six wooden plant essential oils (EOs; Illicium verum, Gaultheria fragrantissima, Bursea delpechiana,Croton anisatum, Cinnamomum cassia and Aniba rosaeodora) and their major compounds (trans-anethole, methyl salicylate, trans-cinnamaldehyde, linalool and linalyl acetate) identified from gas chromatography (GC) and GC-mass spectrometry were tested for adult repellence and pheromone antagonism using Y-tube essay and oviposition deterrent effect using no choice test against adzuki bean beetle (ABB), Callosobruchus chinensis L. EOs of I. verum and C. anisatum as well as their common major compound, trans-anethole were found effective repellents having high degree of pheromone antagonistic and oviposition deterrent activity. Methyl salicylate, the major compound of G. fragrantissima EO (which was only the pheromone antagonist) showed high degree of repellency, oviposition deterrence and pheromone antagonistic effect in higher concentration. From this study, EOs of I. verum and C. anisatum and their common major compound trans-anethole as well as the major compound of G. fragrantissima, methyl salicylate can be screened as eco-friendly management agents against C. chinensis in stored legumes if slow releasing formulations are prepared by future efforts.

In general, the larvae of Protaetia brevitarsis is raised on fermented oakwood sawdust. Many attempts have been to changes components sawdust, such as using other tree instead of oakwood or adding another material. Like this some study exists, about 'component' of sawdust, however little is known of relation between larval growth and quality of sawdust. The phenomenon-big particle size sawdust flows up on excrement from the larvae-is occurring in used sawdust for raising the larvae. This study was conducted to observe the larvae and adult of Protaetia brevitarsis act according to each sawdust particle size. We conducted choice experiments with Protaetia brevitarsis, which were offered sawdust differing in particle size. Females preferred to lay their eggs on the fine particle sawdust. Also in the case of larvae, the more fine sawdust particle has the more high level of preference, and so is hatchability. Especially larvae tend to be slanted toward finer sawdust. Growth rate of the larvae was shown the highest level in the finest sawdust just as others do. These results show that fine sawdust particle size is advantage for larval development and oviposition.

The longevity and fecundity of Naranga aenescens adult were investigated at temperatures of 15.0, 17.5, 20.0, 22.5, 25.0, 27.5, 30.0, and 32.5°C. Adult longevity was influenced by temperature but there was no temperature effect of fecundity. Longevity decreased with increasing temperature: it was highest at 15°C (22.2 ± 3.51 days) and lowest at 32.5°C (6.2 ± 0.32 days). Total fecundity was highest at 25°C (1693 eggs) and lowest at 15.0°C (1115 eggs). The oviposition period was longest at 20°C (8.8 ± 0.57 days) and shortest at 32.5°C (4.1 ± 0.21 days). The oviposition model presented in this study consisted of two reproductive components (total fecundity and age-specific oviposition rate) and a survival component (age-specific survival rate). The relationship between adult developmental rate and temperature was described by the Logan 6 model (r2=0.98, p<0.0001).The age-specific survival rate was explained by a exponential function (r2=0.98, p<0.00001). The age-specific cumulative oviposition rate was described by the two-parameter Weibull function (r2=0.95, p<0.00001). Temperature-dependent fecundity was estimated using the Briere-2 model (r2=0.91, p<0.0125). Daily egg production of N. aenescens in relation to adult age and temperature was estimated.

The spotted-wing drosophila (SWD), Drosophila suzukii (Diptera: Drosophilidae), originally distributed across a few Asian countries, including South Korea, has invaded North America and Europe, but is absent from Australia. In order to export the South Korean grape cultivar Campbell Early to Australia, its potential to serve as oviposition and development medium for SWD must first be determined. In this study, we determined the oviposition and development potential of SWD on Campbell Early, after elucidating the SWD life cycle and establishing an artificial diet-based mass-culturing system. An investigation of the life cycle under five temperature regimes (16, 19, 22, 25, and 28°C) showed that the durations of the egg, larval, and adult stages were shorten when temperature was increased from 16, 19, 22, 25, and 28°C, but pupal duration was shortest at 25°C and extended again at 28°C. A test of oviposition and development potential of SWD on Campbell Early grape clusters showed oviposition of 30.8 ± 6.8 eggs per cluster of injured grapes and 157.7 ± 16.2 eggs on a culture dish of artificial diet. However, in a similar experiment using uninjured grape clusters, only a single egg was deposited on the grape skin, which soon dried. In light of these results, newly harvested grapes left at vineyards during daily harvests are unlikely to serve as an oviposition and development medium for SWD, as long as the grapes remain uninjured.

매실의 낙과 피해를 일으키는 복숭아씨살이좀벌은 경기도, 충청남북도, 전라남북도 지역의 매실과 살구, 그리고 중국산 복숭아 종자에서 확인되었다. 복숭아씨살이좀벌은 땅에 떨어진 핵과류의 핵 속에서 유충 상태로 월동하고, 노숙유충은 4월 중순까지, 번데기는 3월 하순에서 4월 하순까지 관찰되었으며, 성충은 4월 하순~5월 상순 사이에 90% 이상이 우화하는 것으로 조사되었다. 암컷 성충은 핵이 경화되기 이전 씨앗이 액 상일 때 과실 씨앗의 외피 바로 밑에 산란하였으며, 산란관의 길이가 5 mm를 넘지 않아 산란이 가능한 매실의 크기는 직경이 2 cm를 넘지 않았 다. 하나의 과실에 최대 5개의 알이 관찰되었으며, 알 기간은 2일을 넘지 않았다. 부화한 유충은 먼저 고형화되는 배로 이동하여 이를 섭식하면서 성장하는데 이 과정에서 유충들 사이의 경쟁으로 인해 한 마리의 유충만 생존하였다. 6월 상순경 피해를 받은 과실의 대부분이 부패증상을 보이 며 낙과하였다. 땅에 떨어진 과실의 핵 속에서 이듬해 봄까지 노숙유충으로 월동하였다.

The influence of three temperatures, 20℃, 25℃ and 35℃ and four relative humidities (RH), 30-35%, 50-55%, 70-75%, and 90-95% on oviposition and development of Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) on azuki bean seeds was investigated. One day old five males and five females of C. chinensis were introduced to 30 gm azuki bean seeds for each combination of temperature and RH, and replicated ten times. The introduced adults were allowed to lay eggs for 72 hours. Temperature significantly influenced on oviposition with increased number of eggs at 30℃, relative humidity, however, had no effect on egg laying. Development time was affected by both temperature and relative humidity. Development time was shortest at 30℃ and 70-75% RH. Adult emergence success rate was affected by both temperature and RH; increased level of temperature and RH increased emergence rate of adults. The results suggest that C. chinensis is much likely to cause greater damage to azuki bean seeds stored at 30℃ and 70-75% RH.

최근 국내 포도원에서 과실 표면에 구름모양의 흰색 얼룩이 자주 발견되고 있으나, 그 원인에 대해 명확히 알려진 바가 없어 방제대책을 세우지 못하고 있는 실정이다. 흰색의 달무리 반점은 포도 과실 표면에 부정형으로 퍼져 있으며, 그 중앙에는 총채벌레가 산란할 때 만들어진 작은 구멍의 상처가 남아 있다. 이 상처부위는 시간이 지나면서 코르크화되고 표피세포와 분리되어 딱지로 남거나 떨어져 나가게 된다. 이러한 증상은 총채벌레의 섭식이나 노린재의 흡즙에 의한 상처와는 구별된다. 산란구멍에서 발견한 총채벌레 알껍질에서 DNA를 추출하여 ITS2 부위의 염기 서열을 PCR-RFLP 방법으로 분자동정을 실시한 결과 꽃노랑총채벌레의 것으로 확인되었다. 미토콘드리아 COI 염기서열은 이러한 분자 동정 결과를 재확인하여 주었다. 본 연구결과는 포도 과피에 꽃노랑총채벌레 산란에 의해 유발되는 독특한 피해 증상에 대한 정확한 정보를 제공하며 포도원에서 이 해충에 대한 방제전략 수립에 도움이 될 것으로 여겨진다.

This study was conducted to determine the oviposition preference and development of adzuki bean weevil, Callosobruchus chinensis (L.) (Coleoptera: Bruchidae) on five different leguminous seeds a) cowpea, b) white kidney bean c) soybean cultivar Daepung d) mung bean and e) azuki bean. A multiple choice test and a nochoice test were conducted to record the number of eggs laid, total developmental time, number of adults emerged, and adult longevity of C. chinensis on the five different leguminous seeds. Both choice and no-choice tests revealed cowpea seed as the most preferred oviposition substrate. Total developmental time from egg to adult stage ranged from 28 to 31 days; shortest of that on the cowpea followed by adzuki and mung bean seeds. Successful development of C. chinensis was not witnessed on soybean or on kidney bean. Rate of adult emergence was higher on adzuki and mung bean seeds with longer adult longevity of those emerged from adzuki bean and cowpea. Higher egg laying preference and shorter developmental time with longer adult longevity of the beetle on cowpea probably signifies importance of physical as well as chemical attributes of the seed as an oviposition substrate other than evolutionary history of the insect. Cowpea seeds can possibly be used as a pull stimulus either as a trap crop or as a stimulant in the mechanical traps against C. chinensis where other leguminous host crops are grown as main crops.

썩덩나무노린재(Hemiptera: Pentatomidae)의 발육을 15, 20, 23, 25, 30℃에서 조사하였다. 종령기까지 발육을 완성한 온도는 20℃와 23℃이었다. 20℃와 23℃에서 약충기간은 각각 46.9일과 35.5일이었으며, 1령 기간이 가장 짧고 5령기간이 가장 길었다. 실험에 사용한 최저 온도인 15℃에서는 2령까지, 30℃에서는 4령까지 발육하는 동안에 모든 개체가 사망하였다. 암수 성충의 수명은 20℃에서는 각각 53.6일과 45.3일이었고, 23℃에서는 각각 50.1일과 68.1일이었다. 암컷 당 총 산란수는 20℃에서는 135.8개, 23℃에서 242.9개이었다. 알에서부터 성충우화까지 필요한 평균 적산온도(발육영점온도 13.94℃ 적용)는 20℃와 23℃에서 각각 314.5 일도와 367.8 일도이었다. 그 외에 약충의 각 령기별 발육기간과 사망율, 산란전기간과 산란후기간, 산란횟수별 산란수, 알에서 산란전기간까지의 적산온도 등이 조사되었다.

We investigated the duration of laying worker oviposition and egg-laying behavior in three queenless colonies of A. cerana by in situ video recording. Egg load was determined by dissecting laying workers in September 2012. Egg size, length and breadth, shape index and egg elongation were calculated. To determine the number of eggs laid by laying workers per cell at 24, 53, 74, 120 and 171 hours was also monitored. To estimate the number of eggs per cell per week, a small comb was squeezed between two frames. The combs were collected at given hours and weekly to count the number of eggs, respectively. The results showed that the duration of oviposition of laying workers on average was 109.2 ± 67.5 seconds per cell. During oviposition, egg-laying workers showed two types of behaviors, viz; a still phase, where the egg-laying workers did not move, and a recovery phase, where the egg-laying workers vigorously wagged their abdomens after oviposition. The results showed that on average, 4.0 ± 5.1 of worker eggs per cell per week was recorded. The highest number of eggs was recorded at 120 hours compare to at 24, 53, 74 and 171 hours. Three different shapes of the eggs namely oval, elongated long and elongated curve shaped was laid by workers. The results showed that the laying worker carried 1 to 4 mature eggs in her ovaries and may lay from one to four per oviposition. In conclusion, the laying worker shows a still and a recovery phase during and after laying the eggs. The laying workers retain 1 to 4 eggs in their ovaries. The breadth of eggs is strong positive relationships with length. One worker cell can accumulate up to 33 eggs in queenless colonies.

The adzuki bean borer, Ostrinia scapulalis (Lepidoptera: Crambidae), is one of serious insect pests against the red bean, Vigna angularis. Adult emergence, mating and oviposition behaviors of the insect were investigated in a constant condition of 25±1℃, 15L:9D (light: dark) photoperiod, and 60±10% relative humidity, for the purpose of collecting fundamental data for elucidation of adult biology and female sex pheromone. The circadian rhythmic behaviors were observed at 30-min interval during the experiments, and the data was analyzed at one-hour interval. Adult emergence was observed for 15 hours from 2 hours before lights-off to 4 hours after lights-on in a row, and ca. 90% emerged in scotophase. Adults did not mate within the emerged scotophase and the following photophase. Mating was observed only in the scotophase, and ca. 95% of mating occurred for 5 hours from 2 hours after lights-off to 2 hours before lights-on. The result was different from the main mating time in the previous reports. Mean mating rate at 0- to 4-day-old pairs was ca. 50%. It took almost one day from mating to oviposition, but oviposition 2 days after mating was observed in 0-day-old adult pairs. Adult pairs older than 7-day-old could not mate.

Ascotis selenaria is one of most important pest of geometridae moths in citrus orchards causing citrus fruit damages in jeju, Korea. Oviposition site had not been found on citrus tree, but was witnessed at the net adjacent of vinyl covering a green house in 2007. And then, A. selenaria larva highly clumped on the citrus(Shiranui : [C. unshiu × C. sinenesis] × C. reticulata) tree near the oviposition site and the ID(Index of Dispersion) value was 6.81. However, dispersion of A. selenaria larva was not clearly clumped in field citrus(C. unshiu) during 2008 and 2010. The value of GI(Green’s index) was 0.0179, 0.0208, and 0.0064, respectively. Those were so low that A. selenaria larva were distributed almost randomly in field citrus orchard. Consequently, it was assumed that A. selenaria female oviposited on a tree like Cryptomeria japonica surrounding a citrus orchard in field and hatched larva moved to citrus tree by drifting with winds.

The oriental tobacco budworm, Helicoverpa assulta, is an oligophagous insect feeding on a few species of Solanaceae plants, including Nicotina tabacum. This study investigated the relationship between H. assulta and plants, focusing on oviposition preference and larval performance of the specialist in host and non-host plants. (1) In choice experiments, N. tabacum and Phaseolus vulgaris were put in a cage with 5 females and 10 males. Most of eggs (ca. 98%) were layed in N. tabacum, whereas few eggs in P. vulgaris. (2) In non-choice experiments, N. tabacum, Datura stramonium, or P. vulgaris was individually put in a cage with 3 females and 6 males, resulting that N. tabacum was the most preferred host, followed by D. stramonium and P. vulgaris. Oviposition was delayed about 2 days in P. vulgaris (a non-host plant) compared to the two host plants. (3) Larval performances were also compared with the three plants. Larval growth was better in N. tabacum followed by D. stramonium and P. vulgaris, High mortality (ca. 100%) of larvae was observed in P. vulgaris, suggesting toxic component(s) derived from the non-host plant might cause the death. The ovipositional avoidance in P. vulgaris is current being investigated to search for the oviposition deterrent, which could be useful for the environment-friendly pest management of H. assulta.

참딱부리긴노린재의 생물학적 특성을 조사하기 위하여, 온도 20±1, 25±1, 30±1, 35±1, 37.5±1, 40±1℃, 습도 80±10%, 광주기 16:8(L:D)에서 발육실험을 실시하였다. 그 결과 알부터 성충우화까지의 발육영점온도는 14.8℃, 유효적산온도는 399.1일도였다. 35℃에서 조사한 결과를 정리하면 다음과 같다. 알 기간은 5.6일, 부화율은 81.1%, 약충 기간은 14.3일(1령 3.2, 2령 2.2, 3령 2.7, 4령 2.7, 5령 3.6일)이었다. 암컷 성충의 수명은 33.8일, 산란기간은 29.2일이었다. 총 산란수는 111.2개, 하루 최대산란수는 14.8개(7일차)였다. 알과 약충은 37.5℃까지만 발육하고 40℃에서는 발육하지 못했으며, 40℃에서 총 산란수는 22.1개였다. 참딱부리긴노린재 1령, 3령, 5령, 성충의 담배가루이 번데기 하루포식량은 각각 1.9, 7,3, 18.7, 29.5마리였다.

 ,  , An unknown planthopper was discovered in 2010 in an apple orchard in Sinpoong-myun, Gongju-si and in a blueberry orchard in Deogsan-myun, Yesan-gun. This pest have arrived 4 or 5 years ago and the population density then rapidly increased. It was identified as Ricania sp. by Dr. Murray J. Fletcher. Adult Ricania sp. was found on 26 plants including blueberry, grape, peach, sumac, japanese angelica, jujube, kiwi, Rubus coreanus, apple, apricot, plum and chestnut. Females lay eggs on 1-year-old branches. The mean number of eggs laid on apple and plum were 18.7 and 15.3, respectively. The mean length of egg masses on apple and plum were 12.35 and 11.45 mm, respectively. The distance between eggs laid on apple and plum branchs were 1.3 and 1.5 mm, respectively. The mean number of eggs in the same length of egg mass on apple and plum branchs were 17.1 and 13.3 , respectively.

The ovarian development and oviposition response of two vector beetles of pine wood nematode, Monochamus alternatus and M. saltuarius, was investigated. The ovary structure of two beetles was investigated by means of light microscopy. The ovary of both beetles was composed of two ovaries that are connected by a common oviduct. Each of the two ovaries was consisted of twelve ovarioles, the functional units of female oogenesis. The ovary type was meroistic telotrophic with nurse cells and oocytes in the tropharium. Ovarian development of two vector beetles was completed at 12-14 days after maturation-feeding pine twigs. Aspect of accumulation of vitellogenins in the ovary of two vectors showed difference in developmental stages and major yolk proteins, differently from vitellogenin. To investigate oviposition performance of two vector beetles, we provided P. thunbergii and P. koraiensis bolts for egg laying and collected emerged adults from each pine bolts in the next year. M. saltuarius females made more oviposition wounds and entrance hole of larvae than M. alternatus on pine bolts. We also investigated whether two beetles can transmission of Bursaphelenchus xylophilus and the European type of B. mucronatus via oviposition to noninfected dead pines. 48-83% from newly emerged adults of two beetles were carried two species nematode. This results suggested that two species beetles can transmit nematodes through oviposition performance and transmitted nematodes successfully propagate in non-infected dead pines.

Potato tuber moth (PTM) has been a destructive pest on potato in the fields and strores through tropical to subtropical area. According to global warming, PTM distribution limit in Korea is facing north gradually. Recently, potato fields damaged by PTM are reported intermittently in the midsection of a country and southward. To secure a sufficient number of insect with equal growth stage is a prerequisite for investigating bionomics and developing new agrochemicals to control pests. In 2009, we collected PTM larvae first from the potato fields in Miryang and Gimcheon, and then placed them into transparent hexagonal rearing cage (30×30×30cm) with providing potato tubers as a food till emergence. A transparent cylindrical rearing cage (Φ15×30cm) erected vertically before putting emerged adults, and covered upper opening with net (50 mesh). To get eggs, we placed a filter paper (Φ11cm, called ‘oviposition paper’) on the net and sprinkled sugar solution. The oviposition paper laid eggs was stored in 10℃ refrigerator and changed with new one every 1~2 day interval. The developmental period of eggs to hatch was 5.2±0.7 days at 23±2℃, L16/D8 condition. Although adults laid eggs continuously for two weeks, second to fifth days after emergence showed highest oviposition, which suggested the most efficient periods for collecting sufficient eggs. A hatchability of eggs laid within 10 days after emergence was over 90%.