The present study was undertaken to estimate the antibacterial effect of a combination of C. rhizoma,L. Flos, and P. japonica (1:1:1) extracts (CLP1000) and a combination of the herbal extract mixture and dioctahedral smectite (CLPS1000) against murine salmonellosis. At the concentration of CLP1000 and CLPS10000.5 mg/ml, the antibacterial effect was not showed on Salmonella typhimurium (S. typhimurium). On the other hand,the antibacterial effect against S. typhimurium was observed at the concentration of CLP1000 and CLPS1000 1.0 mg/ml. Oral administration of Smectite, CLP1000, and CLPS1000 at the dose of 10 mg/ml showed a therapeutic effect for S. typhimurium infected BALB/c mice. The mortality of Smectite, CLP1000 and CLPS1000-treated mice was 90%,90%, and 70% at 12 days, respectively, while that of untreated mice was 100% at 9 days after a lethal dose of S. typhimurium infection. The results of our study strongly indicate that CLPS1000 has potential as an effective of salmonellosis.

백작약 분말을 떡과 국수에 첨가(0, 1, 3, 5%)하여 저장기간별로 이화학적, 관능적, 미생물학적 특성을 검토하여 저장성 및 제품 특성을 살펴보았다. 수분은 저장 기간동안 감소하였고, 무첨가군이 첨가군들에 비해 보다 급격한 감소를 보였다 이는 백작약 섬유소의 수분 보유력 때문으로 여겨진다. 떡의 밝기의 기준인 L 값은 첨가량이 증가할수록 감소하였으며 저장 기간동안 증가하였다. 적색도 a값과 황색도 b값은 백작약 분말을 첨가할수록 증가하였다. 백작약 분말 첨가 국수의 경우 밝기의 기준인 L값은 첨가량이 증가할수록 감소하였고, 저장기간 동안에도 감소하였다. 황색도 b값은 첨가량이 증가할수록 증가하였다(p〈0.05). 백작약 분말 첨가 떡과 국수 모두 무첨가군에서 총균수가 첨가군보다 더 급격하게 증가하였으며 백작약 분말 첨가량이 많을수록 미생물의 증식 억제효과가 큰 것으로 나타났다. 무첨가 떡과 국수를 48시간 저장 후 총균수는 1.0×103 CFU/g에 달한 반면 5% 첨가군은 96시간 이후 그 수준에 달하였다. 떡과 국수의 색, 향, 맛, 쫄깃한 정도, 전반적인 품질에 대한 관능 검사 결과 백작약 분말 첨가 떡이 무첨가군에 비해 선호도가 높았다(p〈0.05). 백, 국수 모두 촉촉한 정도는 제조 후 24시간 이전에는 무첨군이 더 높은 값을 보였으나 그 이후에는 첨가량이 증가할수록 더 높은 값을 보였다. 백작약 분말 첨가떡과 국수 모두 색은 3% 첨가군의 기호도가 높았으며 향, 맛, 전반적인 품질은 1% 첨가군을 선호하였다. 이상의 결과로 보아 백작약 분말 첨가는 떡과 국수의 기호성을 높이고 저장기간을 연장시키는 가능성을 확인하였다.

Paeonia japonica is a perennial flowering plant used in traditional medicine therapy. The purpose of this study was to investigate the effect of water extract and solvent fractions obtained from P. japonica on anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities in YD-10B cells, human oral squamous carcinoma cell line. Water fraction revealed the highest extraction yield at 11.44% (w/w). Anti-oxidative activity was the highest in ethyl acetate fraction (85.13%). In the thrombin inhibitory activity test, ethyl fraction was the highest, with a value of 87.54%. Release and activation of MMP-2/pro-MMP-2 ratio in thrombin-treated YD-10B cells were significantly inhibited in the ethyl acetate fraction. At a concentration of 120 ㎍/㎖, water extract and solvent fractions of P. japonica inhibited cell proliferation in YD-10B cells except water fraction. Pro-apoptotic effect on human oral squamous carcinoma cell using the Bax/Bcl-2 ratio analysis was higher in water extract than other fractions. These findings suggest that the ethyl acetate fraction of P. japonica potentiates a promising antioxidant, anti-thrombin and anti-invasive agents.

Paeoniae radix has been widely used for its anti-allergic, anti-inflammatory and analgesic effects, and demonstrated to have anticonvulsant, memory enhancing and anxiolytic activities. The present study was performed to examine the protective effect of methanol extract of Paeoniae radix (PR) from Paeoniae Japonica Miyabe et Takeda (Paeoniaceae) on hydrogen peroxide (H2O2)-induced neurotoxicity using cultured rat cerebral cortical neuron. H2O2 produced a concentration-dependent reduction of neuronal viability, PR, over a concentration range of 10 to 100 μg/ml showed concentration-dependent decrease of the H2O2(100 μM)-induced neuronal cell death, as assessed by a 3-[4,5-dimethylthiazol-2-yl]-2,5-di-phenyl-tetrazolium bromide assay and the number of apoptotic nuclei, evidenced by Hoechst 33342 staining. PR (100 μg/ml inhibited 100 μM H2O2-induced elevation of the cytosolic Ca2+ concentration ([Ca2+]c), which was measured by a fluorescent dye, flue-4 AM. PR (50 μg/ml inhibited glutamate release into medium induced by 100 μM H2O2, which was measured by HPLC, and generation of reactive oxygen species (ROS). These results suggest that PR may mitigate the H2O2-induced neurotoxiciy by interfering with the increase of [Ca2+]c, and then inhibiting glutamate release and generation of ROS in cultured neurons.