큰느타리버섯 수확 후 배지를 이용한 유기퇴비를 제조하여 퇴비 원료로서의 이용가능성에 대해 실험을 진행하였다. 퇴비의 발아능력을 검증하기 위해 오이, 무, 상추, 배추를 파종한 후 발아율과 발아지수를 측정하였으며 미강과 미생물제의 첨가비율이 높을수록 높은 발아율을 나타내는 것을 확인할 수 있었다. 오이와 무의 발아지수는 전체적으로 높았으며, 상추의 경우 SMS 100%와 미강 5% 첨가구만 발아지수 70을 넘었고 배추는 미생물제 5% 첨가구에서 가장 높은 발아지수를 나타내었다. 또한 상추를 이용하여 SMS 상토 제조비율과 생육특성을 조사하기 위해 생육시험용 상토 제조비율은 일반상토 100%와 SMS 10%, SMS 20%, SMS 30%, SMS 50%, SMS 70%, SMS 100%로 제조하여 상추묘를 이식하였다. 먼저 상토재료의 이화학성 분석을 시행하였으며 SMS 첨가량이 높을수록 질소전량이 높아지는 것을 확인할 수 있었다. 하지만 상추의 SMS 처리구와 일반상토에서의 생육정도를 비교하였을 때, 중량은 SMS 10% 43.9 g, 일반상토 46.4 g로 비슷하였으며 엽록소 함량도 SMS 10% 32.8, 일반상토 SMS 10% 32.8로 같았고 나머지도 비슷한 수치를 나타내었으나 총장에서 SMS 10% 14.6 cm, 일반상토 16.8 cm로 일반상토가 더 큰 것으로 확인되었다.

생육이 빠르고 중형인 큰느타리버섯 품종을 육종하기 위하여 육종모본 24×46과 KNR2539를 단교배하여 소규모 시험재배하여 생육소요일수(15.4일)와 수량(81.5 g/850 cc), 품질(7.5)을 기준으로 17×15계통을 선발하였다. 선발된 계통을 ‘애린이6’라고 명명하고 대량재배로 큰느타리2호와 생육특성을 비교하였다. 병당수량은 ‘애린이6’가 76.0 g으로 대조품종 61.4 g의 113% 수준이었다. 품질의 경우 ‘애린이6’는 6.8, ‘큰느타리2호’는 5.7로 나타났다. 생육소요일, 수량, 품질은, 독립 t test로 분석한 결과 통계적으로 유의성을 보였다. 갓의 명도(L)에 있어서 ‘애린이6’는 61.7로 대조품종의 58.3보다 높아서 밝은 색을 띄었다. 고유성에 있어서는 URP2와 URP11에서 대조품종과 신품종이 다형성을 보였고, 대치배양에서도 뚜렷한 대선이 관찰되었다.

In this study, we investigate the potential use of persimmon peels (PP) in mushroom culture medium for the production of functional mushrooms. Pleurotus eryngii was cultivated in medium supplemented with PP (SMPP) at the following concentrations: 0% SMPP (control), 5% SMPP, 10% SMPP, 15% SMPP, 20% SMPP, or 30% SMPP. The total polyphenol content, DPPH radical scavenging ability, ABTS cation scavenging ability, and reducing power of P. eryngii cultivated in SMPP were investigated. P. eryngii cultured in 20% SMPP produced the highest values for all four measurements. Total polyphenol content, DPPH radical scavenging activity, ABTS cation scavenging ability, and reducing power all increased upon the addition of PP. Based on our results, we can conclude that persimmon peels are a highly valuable supplement for functional mushroom culture medium.

King oyster mushroom (Pleurotus eryngii), an improved species of oyster mushroom, is a popular ingredient in Asian cuisine. Spleen cells were treated with various concentrations (0, 5, 10, 50, 100, 250, 500, and 1,000 μg/mL) of king oyster water extracts (KOWE); then, the proliferation of the cells was measured 24, 48, and 72 h after each treatment. Also, type 1 T helper cytokine productions (TNF-α, IFN-γ, and IL-2) were measured in activated macrophage by KOWE in seven concentrations. Under the condition of its 50, 100, 250, and 1,000 μg/mL for 48 h, the proliferation of cells was increased. However, there was no significant fluctuation in the spleen cells proliferation for 24 and 72 h-long KOWE exposure. To determine cytokine (TNF-α, IFN-γ, IL-2) productions of type 1 T helper cells, macrophage was stimulated by KOWE for 48 h. Treatment of KOWE gave a rise to the levels of TNF-α and IFN-γ, but not in that of IL-2 productions. These results suggest that king oyster mushroom water extracts may be beneficial for enhancing immune functions in its high concentration.

Pleurotus eryngii, an edible white-rot fungus, is cultivated widespread in Eurasia, northern Africa and China. It occupies the second position in the world mushroom market. Despites the importance, the small numbers of studies have been done. To promote the availability of P. eryngii, it is important to know the genome and organization of the fungus. In this study, the whole genome sequence of P5 monokaryon from P. eryngii KNR2312 strain was sequenced using Next Generation Sequencing (NGS) strategy. Identified 222 SSR markers based on newly known genome information and various type of markers used to construct the map consisted of 12 linkage groups (LGs) spanning 1047.8 cM. Using composite interval mapping, 71 quantitative trait loci (QTL)s were identified on 12 linkage groups (LG) for nine traits such as yield, quality, cap color and earliness in four different populations. Clusters of more than five QTLs for various traits were identified on three genomic regions on LG 1, 7, and 9. The largest cluster was identified on LG 1 in the range from 65.4 to 110.4 cM. We performed the genes prediction analysis responsible for yield in the LG1 genomic region with highest logarithm of the odd (LOD) scores The identified genes were involved in biomass degradation and synthesis and signal transduction. However, the region was wide to identifie the genes crucial role in important trait, we need to narrow down. To improve the resolution of QTL mapping, we enlarge the populations crossing with additional 205 monokaryons. Find mapping of QTLs will increases the accuracy and efficiency of interpret the genomic region and enhance the usefulness of genomic information. [Supported by a grant from the IPET (213007-05-1-SBI30), MIFAFF, Republic of Korea.]

In this study, we attempted to find alternative materials to rice bran (Japonica in Korea) such as rice bran pellet (Indica in Pakistan), corn distillers dried grains with solubles, sesame oil meal, and kapok meal for bottle cultivation of Pleurotus eryngii. Among all treatments, the total carbon content of the mixed substrate was the highest in T1 and the total nitrogen content was the highest in T4. The C/N ratio of T2 was comparable to that of the control and was higher than that of other treatments. The diameter of the pileus was the largest in T1 and T2, and the length of the stipe was the largest in T1 and T2. The yield and bio-efficiency of the fruit body were higher in T2 than in the other treatments, and were similar to that of the control plot. Therefore, the findings of our study suggest that rice bran pellet (Indica in Pakistan) could be a suitable alternative to rice bran (Japonica in Korea) for the cultivation of P. eryngii.

This study was conducted to investigate the effects of Kojongsi persimmon peels as a mushroom substrate on antioxidant activity of Pleurotus eryngii. The Pleurotus eryngii was growth on the medium with 0, 5, 10, 15, 20, 30, 40, 50% Kojongsi persimmon peels. The Pleurotus eryngii was extracted into ethanol and their antioxidant activities were analyzed. The total polyphenol content was highest in the 20% Kojongsi persimmon peels treatment (40±0.38 mg of gallic acid equivalents/100 g) than 0% Kojongsi persimmon peels treatment (21 ±0.18 mg of gallic acid equivalents/100 g). The 20% Kojongsi persimmon peels treatment showed better DPPH scavenging activity (36.81±0.23% at 1 mg/ml), ABTS scavenging activity (39.03±0.10% at 1 mg/ml) and reducing power (0.3±0.10% at 1 mg/ml) than 0% Kojongsi persimmon peels treatment and another extract ones. Overall, total polyphenol content and antioxidant activities of extracts from Pleurotus eryngii was increased dose dependently and 20% Kojongsi persimmon peels treatment was showed better total polyphenol content and antioxidant activities than 0% Kojongsi persimmon peels treatment and another extract ones.

The Kojongsi persimmon peels is byproduct after production of dried persimmon. This study was conducted to investigate optimal mixing ratio of Kojonsi persimmon peels as a substrate for cultivation of Pleurotus eryngii. The primary nutritive material of substrate for Pleurotus eryngii was corncob, soybean meal, beet pulp, ground corn and bran. Five to forty percent of persimmon peels were mixed with primary nutritive materials as new substrate. The chemical properties of substrate mixed with Kojongsi persimmon peels was similar to the mixture without Kojongsi persimmon peels in T-C, T-N, C/N ratio, and other nutrients. Mycelial growth time on medium with 5 to 30% persimmon peels was similar to the control without persimmon peels as 27 days but that on medium with 40% and 50% persimmon peels was delayed for 10 days. The time of pinhead formations in medium with 5 to 30% persimmon peels was 12 days, the growth time to harvest was 6 days. These results were similar to those of the control without persimmon peels. The sizes of pileus of treatments with 5 to 30% persimmon peels were tend to be smaller compared with the control. The length of stipe of 10% persimmon peels treatment was 80 mm which was shorter than that of the control with 120 mm.

To breed a new Korean Pleurotus eryngii cultivar with high quality and yield, single crosses between 24×46 and KNR2539 were performed, and a new cultivar, 6×13, was selected based on the days to harvest (14.6 days), quality (7.2), and yield (85.9 g/850 cc bottle). The strain was named Aeryni 5 and cultivated on a large scale at the mushroom farms to compare with Keuneutari 2ho. The yield of Aeryni 5 (82.2 g) was 122.7% of Keuneutari 2ho, and the quality of the new cultivar was 7.7 while reference cultivar was 6.3. The yield and quality of the two cultivars were statistically different. The lightness of the pileus of Aeryni 5 (61.7) was greater than that of Keuneutari 2ho by 3.4 points; thus, the pileus of Aeryni 5 looked brighter. PCR with URP2 was used to discriminate between Aeryni 5 and Kenneutari 2ho.

Differentiation of Pleurotus eryngii is laborious and time-consuming tasks especially in mycelial status. For development of a method for differentiation of P. eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotypes. PCR with the each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pair-group method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into 2 clusters. Cluster I and II comprised of 4 and 8 cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivar and strains with 21 allele with a PIC value of 0.9090. These results might be useful to provide an efficient method for the identification of P. eryngii cultivars with separate PCR reactions. (This work was supported by a grant from the Golded Seed Project (213003-04-3-SBY20), MIFAFF, Republic of Korea.]

본 연구에서는 버섯 수확후배지를 바이오매스로 활용하 고자 수행하였으며, 적정 전처리 조건을 구명한 결과는 다음과 같다. 버섯 수확후 배지의 전처리방법 중 알카리 처리가 리그닌 함량을 효과적으로 경감시키면서, 당전환 율이 우수하였고 큰느타리 수확후 배지가 당전환율 80.7%로 가장 우수하였다. 큰느타리 수확후 배지에 1% NaOH를 이용한 알카리 처리시 전처리 온도별 성분분석 결과 120℃처리시에 glucose 25.3%, xylose 14.8%로 유 리당 함량이 가장 높고, 효소에 의한 당전환율도 66%로 무처리구 28.2%에 비해 2.3배 증가되었다. 촉매제 농도 (NaOH 0.5~2.0%)에 따른 당분석결과 glucose 함량은 촉 매제 농도와 관계가 적었으며, xylose와 arabinose는 촉매제 농도가 높을수록 다소 감소하는 경향이었다. 그러나 당전환율은 NaOH 1% 처리시에 66%로 가장 높았다.

본 연구는 지역 특산물인 감과피를 이용한 버섯배지 개 발을 위한 기초자료로서 감과피의 이용 가능성을 검토하 고자 수행되었으며 감과피는 경상남도 산청지역에서 곶감 을 생산하고 남은 것을 수거하여 건조한 후 사용하였고 공시균주는 ASI 2312를 사용하였다. 대조구는 혼합배지:콘코브:톱밥(220:65:15, v/v)을 혼합한 기본배지를 사용하 였고 기본배지 중 혼합배지를 5%, 10%, 15%, 20%, 30%, 40%, 50%의 건조된 감과피 처리구로 대체한 처리 구를 시험구로 사용하였다. 대조구인 기본배지에 비해 시 험구인 5%, 10%, 15%, 20%, 30%, 40%, 50% 감과피 처 리구의 T-N 함량은 감과피 첨가량이 증가할수록 감소하 는 경향을 나타내었으며 T-C 함량은 큰 차이가 없었고 C/ N율은 감과피 첨가량이 증가할수록 증가하는 경향을 나 타내었다. 감과피 첨가량이 균사생장에 미치는 영향을 조 사한 결과 대조구보다 감과피가 첨가된 모든 시험구에서 균사생장이 느리게 나타났다. 감과피 첨가량에 따른 배양 일수는 대조구인 혼합배지에 비해 감과피를 30%까지 첨 가하여도 배양일수와 초발이 소요일수에는 뚜렷한 차이는 없었지만 감과피 첨가량이 증가할수록 배양일이 길어지는 경향을 보였으며 혼합배지에 비해 감과피 첨가비율이 증 가할수록 갓의 직경은 감소하고 대의 길이는 길어지고 직 경은 감소하는 경향을 나타내었다. 수확 후 동결 건조한 큰느타리버섯의 수분, 단백질, 조회분 함량은 대조구와 시 험구에서 유의성 있는 차이를 나타내지 않았지만 조지방 함량은 감과피 첨가량이 증가함에 따라 증가하는 경향을 나타내었고 β-glucan 함량은 대조구에 비해 10%와 15% 감과피 첨가구에서 높게 나타났다. 따라서 대조구와 비교 했을 때 15% 감과피 첨가구는 자실체 수량도 높게 나타 나고 β-glucan 함량도 높게 나타났기때문에 감과피 첨가 비율은 15%가 가장 적합한 것으로 사료된다.

큰느타리버섯의 아미노산을 함량을 분석하고, 70% 메 탄올, 95% 발효주정, 물 추출물의 항산화 활성을 측정하 였다. 아미노산 17종류 분석결과 필수아미노산에서는 체 내 단백질을 구성하는 Phe의 함량이 가장 높았고, 비필수 아미노산에서는 감칠맛을 내는 Glu가 가장 높은 함량을 보였다. DPPH 라디칼 소거능에서는 ASI 2887이 70% 메 탄올, 95% 발효주정, 열수 추출용매별로 34.37±1.19, 34.66±5.46, 40.97±1.65 mg/g으로 소거능을 보였고, 아질 산염 소거능 역시 열수추출물이 다른 추출물보다 소거능 이 높았으며 ASI 2820이 70% 메탄올, 95% 발효주정, 열수 추출물에서 각각 21.45±2.73, 33.32±1.06, 33.31±2.65 mg/g으로 추출용매에 상관없이 높은 아질산염 소거능을 보였다. 폴리페놀 함량도 열수 추출물에서 가장 높은 함 량을 보였고 가장 높은 함량을 보인 것은 ASI 2887로 DPPH 라디칼 소거능과 유사한 관계를 보이는 것을 확인 하였다. 큰느타리버섯은 아미노산과 생리활성 물질의 다 량 함유하고 있어 기능성 식품 및 건강식품으로서의 활용 이 기대된다.

This study was carried out to investigate optimal mixture of persimmon peels for Pleurotus eryngii cultivation. The 5-50% of persimmon peels was mixed with mushroom growing medium for Pleurotus eryngii cultivation. Mycelial growth of persimmon peels treatment were 74-165 mm (28 days) and slower than that of control (treatment without persimmon peels) as 160 mm (28 days). Mycelial growth time of 5-20% persimmon peels treatment were similar to control as 34 days but that 30-50% of persimmon peels treatment were delayed for 17 days. The time of pinhead formations in control and 5-50% persimmon peels treatment were 11 days, the growth time to harvest were 6-7 days. The sizes of pileus of 15% persimmon peels treatment was tend to be smaller compared with control. The length of stipe of 15% persimmon peels treatment was 124 mm which was longer than that of the control with 118 mm. The thickness of stipes of 15% persimmon peels treatments was 48 mm which were tend to be thicker than that of the control with 47 mm. Thirty percent of persimmon peels treatment was better than other treatment comparing with control.

This study was carried out to develope mushroom growing medium for Pleurotus eryngii cultivation using persimmon peels. Total nitrogen, carbon source and protein of commercial cultivation medium used in this study was 1.34, 53.9 and 0.09 %, respectively. The C/N ratio was 40.22. The total nitrogen was increased by increasing mixing ratio of persimmon peels. The carbon source and protein was no significant difference by increasing mixing ratio of persimmon peels. In column test, mycelial growth of 15% persimmon peels treatment was similar with control, but mycelia of 20-50% persimmon peels treatment was growed slowly compared with control. To investigate yields of fruiting body, 15% of persimmon peels was mixed with commercial cultivation medium. The fruiting body of 15% persimmon peels treatment was no significant difference yield compared with control. But, the chemical contents of 15% persimmon peels treatment were the higher than control.

Pleurotus eryngii, an edible white-rot fungus, is widespread in Eurasia and northern Africa. It has become a major cultivated mushroom in Asia, with a current global production rate of approximately 3 × 10 5 metrictons/yr. To improve the quality or productivity through breeding, a genetic linkage map is an important component. In this study, genetic linkage map of the P. eryngii was constructed using 98 monokaryotic progeny derived from dikaryon of parental KNR2312 strain derived from haploid meiotic spores. The whole genome sequence of P5 monokaryon from P. eryngii KNR2312 strain by Next Generation Sequencing (NGS) strategy was used to design the SSR markers. 484 primers pairs were identified by SSR Locator I and tested polymorphism via PCR. A total of 241 loci were mapped using Joinmap 4.0, comprising 222 SSR markers, 2 mating type factors, and the 13 INDEL markers. The map consisted of 14 linkage groups spanning 1003 cM at an average marker interval of 4.2 cM. The mating loci, A and B were mapped on linkage groups 4 and 11, respectively. The established linkage map and the genetic information based on NGS could be used for QTL mapping of agronomic traits, marker-assisted breeding that may ultimately lead to outstanding phenotypic characteristics. [Supported by a grant from the IPET (213003-04-3-SBY20), MIFAFF, Republic of Korea.]

Differentiation of Pleurotus eryngii is laborious and time-consuming tasks especially in mycelial status. For development of a method for differentiation of P. eryngii cultivars, simple sequence repeats (SSR) from whole genomic DNA sequence analysis was used for genotyping and two multiplex-SSR primer sets were developed. These SSR primer sets were employed to distinguish 12 cultivars and strains. Five polymorphic markers were selected based on the genotypes. PCR with the each primer produced one to four distinct bands ranging in size from 200 to 300 bp. Polymorphism information content (PIC) values of the five markers were in range of 0.6627 to 0.6848 with an average of 0.6775. Unweighted pair-group method with arithmetic mean clustering analysis based on genetic distances using five SSR markers classified 12 cultivars into 2 clusters. Cluster I and II comprised of 4 and 8 cultivars, respectively. Two multiplex sets, Multi-1 (SSR312 and SSR366) and Multi-2 (SSR178 and SSR277) completely discriminated 12 cultivar and strains with 21 allele with a PIC value of 0.9090. These results might be useful to provide an efficient method for the identification of P. eryngii cultivars with separate PCR reactions. (This work was supported by a grant from the Gold Seed Project [Supported by a grant from the IPET (213003-04-3-WTI11), MIFAFF, Republic of Korea.]

본 연구는 큰느타리버섯 수확후배지 발효사료에 적합한 생균제를 개발하고 큰느타리버섯 수확후배지 발효사료 급여가 비육한우에 미치는 영향을 조사하기 위하여 수행되었다. 큰느타리버섯 수확후배지 발효사료를 제조하기 위하여 큰느타리버섯 수확후배지로부터 cellulase, xylanase 활성이 우수하면서 Asp. flavus에 대해 항균활성을 나타내는 균주를 선발하여 Bacillus subtilis CS21로 명명하고 버섯수확후배지 발효사료용 생균제로 사용하였다. 사양시험은 13개월령 비육한우 20두를 공시하여 29-30개월령까지 수행하였다. 공시동물은 성장단계(육성기, 비육전기, 비육후기)에 따라 배합비를 조절한 TMR 사료를 급여한 대조구와 30%의 큰느타리버섯 수확후배지 발효사료가 첨가된 TMR 사료(30% F-SMS TMR)를 급여한 처리구로 나누어 수용하였다. 시험기간 동안 총 증체량과 일당 사료섭취량은 대조구보다 30% F-SMS TMR 급여구에서 약간 높게 나타났다. 육질등급에서는 대조구와 30% F-SMS TMR 급여구 모두 1등급 이상 출현율이 100%를 나타내었으나 육량등급의 A등급 출현율은 대조구(57%)가 30% F-SMS TMR 급여구(41.67%)보다 높은 경향을 나타내었으며 C등급 출현율은 대조구(0%)보다 30% F-SMS TMR 첨가구(25%)에서 높은 경향을 나타내었다. 이상의 결과를 종합하면 발효공정을 거쳐 기호성과 저장성이 개선된 큰느타리버섯 수확후배지 발효사료 급여는 육질등급에서는 대조구와 비슷하였지만 증체량은 대조구보다 30% F-SMS TMR 급여구가 높은 반면 육량등급의 A등급 출현율은 낮고 C등급 출현율은 높은 경향을 나타내었으므로 육량등급을 높일 수 있도록 배합사료 조성비를 개선한다면 큰느타리버섯 수확후배지는 비육한우의 대체사료자원으로 이용될 수 있을 뿐만 아니라 사료비절감 효과를 기대할 수 있을 것으로 사료된다.

본 연구는 큰느타리버섯의 베타글루칸 고함유 형질에 관련된 SCAR marker를 개발하기 위해 수행되었다. operon사의 OPA(20개), OPB(20개), OPL(20개), OPP(20개), OPR(20개), OPS(20개) 등 총 120개 primer를 random primer(10 mer)로 사용하여 대립 계통 9종과 베타글루칸 고함유 계통 9 종을 대상으로 RAPD를 이용한 bulked segregant analysis를 실시하여 OP-R03 primer로부터 대립 계통에는 나타나지 않고 베타글루칸 고함유 계통에만 나타나는 특이적인 RAPD 밴드를 얻었다. OP-R03 primer를 이용한 RAPD 결과, 약 91 bp 부근에서 베타글루칸 고함유 계통에 특이적인 DNA 밴드가 관찰되었으며 이 DNA 밴드의 염기서열 말단을 근거로 SCAR 마커로 사용할 specific primer인 OP-R03-1-F와 OP-R03-1-R를 디자인하였다. SCAR 마커 OP-R03-1-F/-1-R primer를 이용하여 PCR을 수행한 결과에서도 91 bp 부근에서 대립 계통과 구별되는 DNA 밴드가 베타글루칸 고함유 계통에서 확인되었으며 random primer인 OP-R03 primer를 이용하여 PCR을 수행했을 때보다 재현성이 높고 진한 DNA 밴드임을 확인할 수 있었다.

본 연구는 지역 특산물인 감과피를 이용한 버섯배지 개발을 위한 기초자료로서 감과피의 이용 가능성을 검토하 고자 수행되었으며 감과피는 경상남도 진주시에 소재한 진주단감원예조합으로부터 건조된 것을 수집하여 사용하 였고 공시균주는 큰느타리 2312를 사용하였다. 대조구는 시판혼합배지를 사용하였고 건조된 감과피 5%, 10%, 15%, 20%, 25%, 30%이 첨가된 시판혼합배지를 시험구 로 사용하였다. 대조구인 시판혼합배지에 비해 시험구인 5%, 10%, 15%, 20%, 25%, 30%의 감과피가 첨가된 시 판혼합배지의 총질소 함량은 감과피 첨가량이 증가할수록 증가하는 경향을 나타내었으며 총탄소와 유기물 함량은 큰 차이가 없었고 C/N율은 감과피 첨가량이 증가할수록 감소하는 경향을 나타내었다. 감과피 첨가량에 따른 시험 관 내에서의 균사생장을 조사한 결과 대조구인 시판혼합 배지보다 감과피가 첨가된 모든 배지에서 균사생장이 느 리게 나타났다. 시험구의 혼합배지 중 5% 감과피가 첨가 된 배지에서 균사 생장이 가장 빨라지만 감과피 첨가량이 증가할수록 균사 생장속도는 느려지는 경향을 보였다. 감 과피 첨가량에 따른 배양일수는 대조구인 시판혼합배지에 비해 감과피를 30%까지 첨가하여도 배양일수와 초발이 소요일수에는 뚜렷한 차이는 없었지만 감과피 첨가량이 증가할수록 배양일이 길어지는 경향을 보였으며 시판혼합 배지에 비해 감과피 첨가비율이 증가할수록 갓의 직경과 두께는 감소하고 대의 길이는 짧아지고 굵기는 굵어지는 경향을 나타내었다. 따라서 감과피를 배지자원으로 이용 하기 위해서는 감과피 첨가량에 따른 혼합배지의 조성비 가 필요할 것으로 생각된다.