흰점박이꽃무지 (Protaetia brevitarsis) 유충은 동아시아에서 식용 및 약용으로 널리 사용되고 있다. 흰점박이꽃무지 수요 증가로 인해 유충의 대량 사육 방법이 필요하지만 좁은 공간에서 대량으로 유충을 사육할 경우 곤충 병원성 질병 감염에 취약해진다. Metarhizium anisopliae는 흰점박이꽃무지 유충에 치명적인 곤충 병원성 곰팡이 중 하나이다. 이를 저해하기 위해 항진균 성분이 함유된 옻나무 (Rhus verniciflua Stokes) 추출액을 첨가하였고, 연구 결과 1% 옻나무 추출액을 첨가한 경우 녹강균에 의한 유충의 치사율이 유의미하게 낮았다. 또한 일반톱밥을 급여한 대조구에 비해 옻나무 추출액 처리구에서 유충의 무게가 더 무거웠고 유충 기간이 짧아 지는 것을 확인하였다. 따라서 이 연구를 통해 곤충 병원성 곰팡이에 대한 옻나무 추출액의 항진균 효과를 확인할 수 있었으며 흰점박이꽃무지 유충 사육 시 옻나무 추출액을 첨가 하여 급여한다면 병원성 곰팡이에 의한 유충의 치사율을 낮추고 증체량을 높이는 데 효과가 있을 것이다.
In this study, antimicrobial and antioxidative activities were investigated using the hot water extracts of Rhus verniciflua Stokes (RVS) in order to develop RVS as a natural antimicrobial and antioxidative material. The antimicrobial activity was not found against five strains of yeasts (Saccharomyces cerevisiae, Saccharomyces pastorianus, Torulaspora delbrueckii, Zygosaccharomyces rouxii, and Zygosaccharomyces bailii) while the activity was confirmed against five strains of bacteria (Bacillus subtilis, Staphylococcus aureus, Bacillus licheniformis, Escherichia coli, and Pseudomonas aeruginosa) at the RVS extract concentration of 0.2-1.0% (w/v) by plate cultural method. In the investigation for 24 hours by turbidometry, the antimicrobial activity showed a relatively high growth inhibition of 79.7% against Bacillus subtilis. The highest growth inhibition of 82.6% was against Bacillus licheniformis at the RVS extract concentration of 2,000 μg/mL. The lowest growth inhibition was 25.7-44.1% against E. coli at the extract concentration of 250 μg/mL and 2,000 μg/mL. The total polyphenol and flavonoid contents of the RVS extract were 42.48 mg/g and 5.42 mg/g, respectively. The highest antioxidative activity at 79.15% was observed at the RVS extract concentration of 0.5 mg/mL by DPPH. Also the antioxidative activity by ABTS demonstrated the highest level at 81.56% at the same concentration. It can be gleaned from these results that the hot water extract of RVS has a great potential to be a natural antioxidant and antimicrobial material against gram positive bacteria(Bacillus subtilis and Bacillus licheniformis).
The current study was carried out to investigate the effect of addition of Rhus veniciflua Stokes oil (RVSO) and black garlic extract (BGE) on the lipid oxidation in Hanwoo (Korean cattle) beef model systems. The RVSO at 0.2% inhibited the TBARS (2-thiobarbituric acid reactive substances) formation when tested in liposome model system. The antioxidant effect of RVSO was further found to be similar to 0.2% butylated hydroxy toluene (BHT) and 0.01% vitamin E. On the other hand, BGE at 0.1% also showed the inhibition of TBARS formation in 4% NaCl-added Hanwoo beef patty and found to have slightly lower (p<0.05) effect than 0.1% vitamin E but higher (p<0.05) effect than 0.1% BHT. Results of this study indicated that both RVSO and BGE possess strong antioxidant effects and help to increase the oxidative stability in Hanwoo beef products.
This study was to invesigate the effect of dietary supplemention of Pozzolan, Chromium-methionine chelate and rhus verniciflua stokes additive feed on growth performance and ultrasound live body characteristics in finishing Hanwoo steer. The animal used in the experiment were a total of 17 heads and weighted 577.09±35.90㎏. The experimental diets were basal hi-lok branded only concentrat and rice straw and 4% Rhus, 0.4% Cramin and 2% Pozzolan, which additice in basal diet. Individually average daily gain was tended to decreased with treated group. Ultrasound back fat thickness and marbling score were not significantly different(p<0.05)between teratment, however Pozzolan additive treat was showed tend to increased. Longissimus muscle area was showed tended to decreased in the Pozzolan treatment.
The purpose of this present study is to measure the changes of regional cerebral blood flow(rCBF) was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) through the data acquisition system composed of MacLab and Macintosh computer. Contractile force was measured with force displacement transducer under 1.5 g loading tension. Rhus verniciflua stokes increased the changes of rCBF in rats, significantly. The rCBF of Rhus verniciflua stokes was decreased by pretreated propranolol, l-NNA and methylene blue. But atropine indomethacin did not change the rCBF. Contractions evoked by PE 0.1μM were significantly inecreased by Rhus verniciflua stokes. Propranolol significantly altered the effect of Rhus verniciflua stokes, but l-NNA, ODQ and indomethacin did not change the relaxation of Rhus verniciflua stokes. These results suggest that Rhus verniciflua stokes can increase the rCBF and relax EP induced contraction of isolated rat aorta, and that is related to sympathetics and nitric
oxide.
The objective of this research was to investigate the effect of immersion in Rhus verniciflua Stokes extract (RVSE) on the total reducing ability (TRA), protein oxidation and myoglobin oxidation of beef steak (Hanwoo longissimus muscle) stored with high oxygen-modified-atmosphere packaging (MAP) (HOMAP, 75% O2+20% CO2+5% N2) and low oxygen-MAP (LOMAP, 0% O2+20% CO2+80% N2) at 4℃ for nine days. RVSE induced TRA (p<0.05), metmyoglobin (MetMb) formation, and the CIE Ho value but reduced the carbonyl content and R630-R580, as an index of the intensity of redness by oxymyoglobin, and the CIE L*, a*, and C* values. HOMAP maintained a lower TRA, MetMb concentration, and CIE H° value but had higher R630-R580 and CIE L*, a*, and C* values compared to LOMAP. Therefore, RVSE induced TRA and protein oxidation stability but reduced myoglobin stability in Hanwoo beef steak. In addition, the effects of HOMAP were opposite those of RVSE.
옻나무의 근삽에 의한 묘목생산에 있어서 적정삽목 시기와 삽수의 절단 여부에 따른 신초발생율과 묘소질을 조사하였다. 삽목시기가 빠를수록 신초는 3-4주 후부터 발생하여 늦은 경향이었으나 삽식 10주 후의 신초 형성율은 높은 경 향이었는데 3월 15일과 25일 및 4월 5일 삽목구는 84% 이상이었으며, 신초는 삽목시기가 늦을수록 각시기별로 고르게 발생하는 경향이었다. 묘소질은 4월 5일과 3월 25일 삽목구가 수장, 주당 분지수, 엽수, 경직경 및 엽면적 모두 가장 양호하였다. 절단하지 않은 삽수로부터 신초의 발생은 2주 후부터 시작되었으나 절단한 삽수로부터는 3주 후부터 시작되어 약 1주일이 늦었다. 그러나 삽식 6주 이후부터 10 cm의 크기로 절단한 삽수의 신초발생율은 급격히 증가하여 10주 후에는 84%이었으나 절단하지 않은 삽수의 신초발생율은 54%로 매우 낮았다. 절단하지 않은 삽수의 묘가 경시적으로는 양호하였으나, 수장, 분지수 및 경직경 등은 통계적인 유의성이 인정되지 않았다.
Allergen-removed-extract was produced from Rush verniciflua by two phase methods. Phase one was high temperature treatment of Rush verniciflua tree to get allergen-removed-extract. Phase two was extraction of solution from phase one product using water or organic solvents. The solutions from above method show high antioxidant activity, anticancer activity, and improvement in lung function, but did not contain urushiol family compounds.
Four olefinic catechols, commonly referred to as urushiol were isolated from the sap of Korean Rhus verniciflua Stokes and had the stronger inhibitory effects on the reverse transcriptase of AIDS. The hexane extract with a inhibitory effects on reverse transcriptase was purified by silica and ODS gel column chromatography. The active compounds were identified by MS and 1H-NMR as 3-[8' (Z), 11' (Z), 14' -pentadecatrienyl]catechol, 3-[8' (Z), 11' (Z)-pentadecadienyl]catechol, 3-[8' (Z)-penta- decenyl]catechol, and 3-pentadecylcatechol. All of these compounds showed strong inhibitory effects on reverse transcriptase of AIDS, in which 3-pentadecylcatechol exhibited the highest activity (IC50 : 10.87 μg/ml).
옻나무의 묘목생산성을 높이기 위하여 근삽에 있어서 BA의 처리방법과 삽식법을 공시하였다. 삽식 당일에 삽수를 1,000 mg/L BA 용액에 1분간 침지처리가 적하법보다 초기에 신초의 발생율은 낮았으나 5주 이후부터 급격하게 증가하여 10주 후에 는 약 85%로 가장 높았으며, 적하법에서는 BA의 처리 간격이 신초의 발생에 큰 영향이 없었다. 2일 간격으로 100 μl의 BA를 적하한 삽수에서 수장이 가장 컸으며 엽수도 많았고, 다음으로 침지처리가 묘소질에 효과적이었다. 분지의 발생은 침지처리구 보다는 적하처리구의 유묘가 삽수와 가까운 지점에서 발생하였다. 평삽이 사삽과 직삽에 비하여 신초의 발생이 약 1주일 빨랐고 신초의 발생도 3주 이후부터 6주까지 급격하게 증가하는 경향이었는데, 삽식 10주 후의 신초발생율은 약 96%로 가장 높았다. 평삽구의 묘가 약 40cm로써 가장 컸으며 평삽구의 묘가 약 40cm로써 가장 컸으며 제 1분지의 발생부위도 낮은 경향이었다. 평삽의 경우 각각 다른 부분에서 발생하는 2~3개의 신초를 각각 분리하여 하나의 묘목으로 생육시킴으로써 묘목의 대량증식에 효과적이었다.