본 연구는 천연물질에서 유래한 반추위 메탄저감용 친환경 첨가제 개발을 위해 각기 다른 종균을 이용하여 발효한 세신 추출물의 항균활성, 항산화활성 및 in vitro 반추위 발효시험을 체계적으로 실시하였다. 접종된 균주들의 성장효율을 알아보기 위해 실시한 생균수측정 결과 L. curvatus NJ40 균주에서 유의적으로 높은 균주성장을 나타냈다(p<0.05). 항균활성측정 결과는 대조구 대비 L. curvatus NJ40 및 L. plantarum NJ45 균주와 발효된 세신 추출물이 병원균에 대한 항균효과를 나타내는 것으로 조사되었다. In vitro 반추위 발효실험에 세신 및 발효 세신 추출물을 적용한 결과, 휘발성 지방산 생성량 대비 반추위 메탄 저감효과가 나타났다. 특히 반추위 미생물 활력 및 사료이용 효율을 대표할 수 있는 휘발성 지방산 생성효율에 대한 부정적 효과 없이, 오히려 휘발성지방산 생성효율을 향상시키면서 반추위 메탄 저감효과를 가져올 수 있는 것으로 나타났다.

This study was carried out to investigate the effect of DDGS hydrolysate (H-DDGS) and rumen-protected lysine-choline complex (RPLC) on milk production and blood metabolites in dairy cows. Feeding trials was performed to latin-square design using the 4 mid-lactational cows for 8 weeks, and treated with T1 (H-DDGS 1.1 kg), T2 (H-DDGS 0.73 kg + RPLC 0.15 kg), T3 (H-DDGS 0.37 kg + RPLC 0.30 kg) and T4 (H-DDGS 0.37 kg + RPLC 0.15 kg) according to the content of protein source. Dry matter intake (DMI) of TMR and average weight showed no significant difference between treatments. The milk production of T1, T2 and T4 were significantly higher than T3 treatment (p < 0.05), and milk/DMI efficiency tend to increase in the T1. Milk components showed no significant difference between treatments, however, the milk protein of T2 increased to 0.15% than T1. Also blood metabolites showed no significant difference between treatments. But T-CHO level numerically represented a lower trend in the treatments of adding to RPLC compared with T1. This result suggests that the high level (1.1 kg) of H-DDGS is expected to improve the feed utilization without the negative impact on weight gain, feed intake and milk production as the lactation stage of dairy cows proceeds, and 0.15 kg of RPLC under the same feeding conditions of H-DDGS may be useful on fat metabolism.

To develop anti-acidosis and anti-diabetes agentsfrom natural products, the inhibitory activities of Brazilian plant extracts against microbial -amylase and -glucosidase were evaluated. Among 100 different ethanol extracts tested, those of Acacia jurema Mart., Anacardium humile A. St.-Hil., Cedrela odorata L., and Guazuma ulmifolia Lam showed good inhibitoryactivities toward both enzymes. In addition, an extract of Plumeria drastica Mart. showed specific inhibition of -amylase, whereas that of Eugenia uniflora L. demonstrated strong inhibition of the enzyme. IC50 values of -amylase inhibition suggested that the extract of A. humile A. St.-Hil., which has been used as an anti-diabetes medicine in Brazil, had potent inhibitory activity. The IC50 for the A. humile A. St.-Hil. extract () was similar to that of acarbose (). This activity of A. humile A. St.-Hil. was not reduced by heat or acid treatment. Moreover, treatment with HCl (0.01 M) for 1 h increased the inhibitory activity from 57.5% to 81.2%. Also, the extract did not cause hemolysis of human red blood cells at levels up to 1 mg/mL. The results indicate that the extract of A. humile A. St.-Hil. is potentially useful as an anti-acidosis and anti-diabetes agent.