Various small molecules can be used to control major signaling pathways to enhance stemness and inhibit differentiation in murine embryonic stem cell (mESC) culture. Small molecules inhibiting the fibroblast growth factor (FGF)/ERK pathway can preserve pluripotent cells from stimulation of differentiation. In this study, we aimed to evaluate the effect of pluripotin (SC-1), an inhibitor of the FGF/ERK pathway, on the colony formation of outgrowing presumptive mESCs. After plating the zona pellucida-free blastocyst on the feeder layer, attached cell clumps was cultured with SC-1 until the endpoint of the experiment at passage 10. In this experiment, when the number of colonies was counted at passage 3, SC-1-treated group showed 3.4 fold more mESC colonies when compared with control group. However, after passage 4, there was no stimulating effect of SC-1 on the colony formation. In conclusion, SC-1 treatment can be used to promote mESC generation by increasing the number of early mESC colonies.

최근 의약적으로 유용한 단백질을 대량 생산키 위한 실현 가능한 방법이 유전자변환 가축의 이용과 관련되어 발전되어 왔다. 이러한 유전자 변환동물은 이종의 단백질을 유즙속으로 분비시키는 생체반응기로서 이용되고 있다. 이러한 전략적 목적을 위해 현재 유전자 변환동물의 생산을 위한 이용에 있어 여러 가지 방법들이 보고되고 있다. 그러나 ES 세포의 사용이 이러한 방법들 사이에서 가장 실질적인 것으로 추정되고 있다. 본 실험에서는 유전자 구축을 위해 사람 황체

In this study, we investigated on antioxidative activity and nitric oxide production inhibitory activity of various solvent extracts of Lespedeza bicolor. The total polyphenol content of the methanol extract was 192.6 mg/g and flavonoid content of the acetone extract was 40.6 mg/g, as the highest content. In DPPH radical scavenging ability, SC50 values of the ethanol and methanol extract were exhibited 0.69mg/ml and 0.89mg/ml, respectively. However, in nitric oxide(NO) scavenging ability, SC50 values of the acetone was exhibited 0.72mg/ml as the highest activity. Moreover, the acetone extract showed strong NO production inhibitory effect in lipopolysaccharide(LPS)-stimulated Raw 264.7 cell. In the cytotoxicity measurement by MTT assay, the extracts were exhibited Raw 264.7 cell viabilities of 92.57~129.04% as nontoxic result in concentration of 65~650μg/ml. As a result, the acetone extract of L. bicolor could be applicable to functional materials for anti-inflammatory related fields.

단분화성 정원줄기세포의 장기간 체외배양 중에 확립되는 다분화능 정원줄기세포는 배아줄기세포와 유사한 특성을 가져 3배엽성 세포로 체외분화가 가능하며 기형종을 형성할 수 있다. 본 연구에서는 선행 연구를 통해 outbred 생쥐(ICR strain)로부터 확립된 다분화능 정원줄기세포의 형질전환 가능성을 확인하며, 배아 내로 주입하여 유전적 키메라를 형성하는 효율을 배아줄기세포와의 비교를 통하여 검증하고자 하였다. 다분화능 정원줄기세포를 넣은 배아로부터 태어

This study was conducted to determine feasibility of production system of Perilla frutescens leaf-stem by fertilizing of Sta-Green in pots. Germination rate of Perilla frutescens seeds collected in 2002 was 7%, also germination rate of seeds collected in 2003 was 62%, while germination rate of seeds collected in 2004 was above 93%. Seed germination rate of Perilla frutescens collected in 2004 were higher than seed gathering in 2002. Especially, plant growth and yield of Perilla frutescens grown in pot(The pots was filled with soil mixtures of Sta-Green and Peat Moss mixed with 40:60 ratio.) was the highest. These results indicate that leaf and stem production of Perilla frutescens can be improved by fertilizing of Sta-Green in pots.

Activated carbon(AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on Leaf and Stem Production of Angelica acutiloba as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated carbon added with 10, suggesting that optimum amount of activated carbon was ranged from 10 to 20%. Growth and enlargement of the root were improved by 10% AC.

This study was conducted to produce effectively more leaves of Angelica acutiloba through year-round production system by using various mulching materials. Soil temperature by covering treatment was the highest in P.E.(Polyethylene Film) mulching and followed by rice straw mulching, especially showing highest temperature in Tr. PE(Transparent Polyethylene Film) from April through May. Water content of soil was higher in P.E. mulching than in non-mulching. Leaf and stem production of Angelica acutiloba through year-round production showed the highest in PE mulching also root production of Angelica acutiloba showed the highest in P.E. mulching. These results suggest that leaf and stem production of Angelica acutiloba can be maximized through P.E. mulching cultivation system.

This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by fertilizing of Sta-Green in pots. The results obtained are summarized as follows. Germination rate of Angelica acutiloba seeds collected in 2003 was 13%, while germination rate of seeds Collected in 2004 was above 91%. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than seed gathering in 2003. Especially, plant growth and yield of Angelica acutiloba grown in pot(The pots was filled with soil mixtures of Sta-Green and Peat Moss mixed with 45:55 ratio.) was the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by fertilizing of Sta-Green in pots and optimizing seed collecting time in greenhouse.

This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by using connect pots in 2003 and 2004. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than those harvested in 2003. Germination rate of Angelica acutiloba seeds collected in 2003 was 10％, while germination rate of seeds collected in 2004 was above 90％. Especially, plant growth and yield of Angelica acutiloba grown in connect pots sized with 4 × 4 × 5cm(length × width × height) were the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by using connect pots and optimizing seed collecting time in greenhouse.

This study was conducted to develop the clonal propagation technique through in vitro culture using basal stem explants in Phalaenopsis hybrid grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when basal stem explants were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 ml/L coconut water, 1 mg/L NAA, 5 mg/L 2iP and 2.5g/L gel rite. PLBs transferred to Hyponex medium were regenerated to plantlets. Plantlets transferred to plastic pots containing spagnum moss were developed and successfully acclimatized under greenhouse. The flower was bloomingly opened in plants regenerated from basal stem explants. The flower was not different from both mother plant and plant induced through clonal propagation of Phalaenopsis hybrid.

본 연구는 고구마 수확시기에 줄기를 예취하여 월동 재배할 수 있는 조건을 구명하여 이듬해에 본포 삽식묘로 활용함으로서 씨고구마의 저장, 파종 및 묘 생산에 소요되는 노력과 비용을 절감하고자 시험을 수행하였던 바 다음과 같은 결과를 얻었다. 1. 줄기묘의 월동재배를 위해서는 터널피복처리에 의해 생존이 가능하였으며 적정 재식시기는 10월 중순이었다. 2. 줄기묘의 재식밀도는 10 3cm(333주/m2 )가 적정하였다 3.줄기묘의 겨울철 생육을 위해서는 최저 5℃ 이상이 필요하였다. 4. 줄기묘의 묘수는 씨고구마에 의해 생산된 묘수에 비해 적었으나 줄기묘의 조기 증식에 의해 묘수를 증가시킬 수 있었다. 5. 줄기묘의 본포 수량성은 씨고구마묘와 차이를 보이지 않았다. 6. 품종간에는 줄기묘의 월동 후 생존율에 큰 차이를 보이지 않았다.

감자의 생장점 배양을 통하여 생산된 소량의 소괴경(microtuber)을 경삽하여 균일하게 생장된 플러그우량묘를 대량생산할 수 있는 기술을 개발하기 위하여 삽수를 정단 및 기부부위로 구분하고 NAA 및 IAA 10 mg /1 용액에 2시간 침지하여 cocopeat, vermiculite, perlite, peatmoss 혼용배지를 이용한 삽목과 온로장 처리 ,포장정식후 괴경형성효을에 대하여 시험한 결과를 요약하면 다음과 같다. 1. 감자의 소괴경 유내 줄기를 이용한 플러그 삽목시 고체배지는 cocopeat, vermiculite, perlite, peatmoss를 이용한 혼합배지에서 플러그의 형성율이 양호하게 나타났다. 2. 고온장일조건(20℃ 이상, 16시간의 일장)에서는 지상부생육 및 지하부발근이 양호하였으나, 외부방치구에서는 발근이 부진하고 복지가 발생하여 소괴경이 형성되었다. 3. 지상, 지하부 생체중은 생장조절제 전처리시 NAA 10mg/1 및 IAA 10mg/1 간에 차이가 없었다. 4. 삽수부위별로는 삽목묘의 초기생육은 초장에서는 비슷한 경향이었고, 그 외 엽수, 근수, 근장 등의 형질은 정단부위묘가 양호하였지만, 포장정식 후에는 큰 차이가 없었다. 5. 삽목묘를 봄재배하여 괴경형성여부를 시험한 결과 초장은 70~80cm, 복지수 10여개, 괴경수는 7~8개가 형성되었다.

구기(枸杞) 신조예취(新梢刈取)가 경엽(莖葉) 및 과실(果實) 수량(收量)에 미치는 영향(影響)을 구명(究明)하고자 1991년부터 1992년 까지 청양종(靑陽種)을 공시(供試)하여 시험(試驗)을 수행(遂行)하였던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 구기자(枸杞子) 생육시기별(生育時期別) 지상부(地上部) 건물중(乾物重)(잎, 줄기) 변화(變化)를 보면 7월(月) 중순(中旬)에 최대치(最大値)를 기점(起點)으로 후기(後期)부터 감소(減少)하고 지상부(地上部) 건물중(乾物重) 중(中) 구기엽(枸杞葉) 6월(月) 하순(下旬)에 최대치(最大値)에 이르다가 急激(급격)히 감소(減少)하였으며 구기경(枸杞莖)은 생육초기(生育初期)부터 완만히 증가(增加)하였다. 2. 착과수(着果數)는 주지(主枝), 2차분지(次分枝)보다 1차분지(次分枝)가 많아 1차(次) 분지(分枝)를 많이 발생(發生)토록 하는 전지(剪枝) 방법(方法)이 연구(硏究)되어야 할 것으로 사료(思料)되었다. 3. 구기엽(枸杞葉)은 5, 6, 7월(月)에 채취(採取)하는 것이 신초장(新梢長), 분지수(分枝數)가 많았고 건조비율(乾燥比率)이 낮아서 품질(品質)이 좋을것으로 사료(思料)되었다. 4. 구기엽(枸杞葉) 채취회수(採取回數)가 늘어날수록 구기자(枸杞子)(과실(果實))수량(收量)은 감수(減收)되었고 구기엽(枸杞葉) 건물수량(乾物收量)은 증가(增加)하였으나 구기자(枸杞子)(과실(果實)) 생산수량(生産收量)을 고려할시(時)는 구기엽(枸杞葉) 채취(採取) 회수(回數)를 3회(回)이내 까지 채취(採取)하는 것이 유리(有利)할 것으로 사료(思料)되었다.