최근 최소한으로 가공된 안전한 식품에 대한 소비자의 수요가 기하급수적으로 증가하고 있다. 이러한 이유로 많은 식품가공 업체에서는 식품안전을 강화하고 유통기한을 연장하기 위한 최소한의 가공공정 중 허들기술(hurdle technology)을 적용하고 있다. 한편, 연구에 따르면 식품에 함유된 병원균을 비활성화하기 위한 공정 및 방법들은 식중독세균들의 스트레스 적응 메커니즘을 촉발시켜 심지어 후속 치료로부터 교차 보호를 준다. 또한, 항생 제와 제초제 사용과 같은 일상적인 농장 관행은 항생제 내성을 가진 병원균의 생성을 초래할 수 있다. 이러한 항생제 내성 박테리아는 식품 처리과정과 관련된 스트레스에 내성을 가질 수 있고 가공 식품에서 생존 할 수 있는 가능성을 높일 수 있다. 이 리뷰에서는 식품 가공과 관련된 스트레스와 항생제 내성의 상관관계에 대해 논의한다. 또한, 항균성 화합물 및 기타 식품 처리 관련 스트레스에 대한 교차 보호 수단으로서 시그마 인자 (sigma factors), SOS 반응 경로(SOS response pathways) 및 유출 펌프(efflux pumps)의 사용과 같은 분자유전학적 기작에 대해서도 논의한다.

본 실험에서 아질산 급성노출은 대왕범바리의 혈액학적 성상 및 혈장성분에 유의적 변화를 나타내었다. 혈액학적 성상인 Hct와 Hb는 아질산 노출에 의한 유의적 감소를 확인하였다. 혈장 성분인 glucose, cholesterol, GPT 및 ALP는 아질산 노출에 의해 유의적으로 변화를 나타냄을 확인 하였다. 본 실험의 결과 아질산 노출 100 mg L-1 이상의 농도는 대왕범바리의 혈액 성상 및 혈장 성분의 유의적 영향을 미치며, 800 mg L-1의 아질산 급성 노출은 대량 폐사를 유발할 수 있으나, 기존 국내 양식 대상종인 Olive flounder, P. Olivaceus는 171.043 mg L-1 (Kim et al. 2018), Yellow tail, Seriola quinqueradiata는 147 mg L-1 (Sugiyama et al. 1991)에 비해 상대적으로 높은 값을 보여 아질산 내성이 상대적으로 강함을 확인할 수 있었다.

The purpose of this study was to investigate the effects of different types of exercise training on neurodegeneration and cognitive function in mice with impaired glucose tolerance (IGT). Thirty-six male C57BL/6 mice were randomly assigned to the control (CO, n = 9) and impaired glucose tolerance (IGT, n = 27) groups. The IGT group consumed 45% high fat diet for 4 weeks and received 40 mg/kg of streptozotocin twice in the lower abdomen to induce IGT. After the IGT induction period, the IGT group was subdivided into IGT + sedentary (IGT, n = 9), IGT + endurance exercise (IGTE, n = 9), and IGT + resistance exercise (IGTR, n = 9). The IGTE and IGTR groups performed treadmill and ladder climbing exercises 5 times per week for 8 weeks, respectively. Fasting glucose and glycated hemoglobin (HbA1c) levels were significantly higher in IGT group than in CO, IGTE, and IGTR groups (p < 0.05). HOMA-IR was significantly higher in IGT group than CO group (p < 0.05). Hippocampal catalase (CAT) was significantly lower in IGT group than in CO group (p < 0.05), while beta-amyloid (Aβ) was significantly higher in IGT group than in CO group (p < 0.05). Hippocampal tau was significantly higher in IGT group than in CO, IGTE, and IGTR groups (p < 0.05). The Y-maze test performance for cognitive function was significantly lower in IGT group than in CO, IGTE, and IGTR groups (p <0.05). These results suggest that IGT induces neurodegeneration and negatively affects cognitive function, while regular exercise may be effective in alleviating neurodegeneration and cognitive decline regardless of exercise type.

지구기후가 이산화탄소 배출 증가로 인해 급격하게 변화하고 있는 추세이고, 이러한 급격한 기후 변화는 농경지 염류직접, 농경지 침수, 고온 및 저온과 같은 농업 기상의 다양한 변화를 야기하고 있다. 여러 환경스트레스 중에서 농경지 침수는 경작지 내 과도한 수분 유입으로 인해 유도되게 된다. 특히 많은 과학자들은 탄소 배출 증가로 인한 기후변화로 인해 집중호우가 지금보다 더 자주 발생할 것으로 예상되고 있기 때문에 농경지의 침수로 인한 농작물의 피해는 점점 더 증가할 것으로 예상하고 있다. 침수로 인한 스트레스에 대한 식물의 취약성 및 저항성 메커니즘에는 중요한 변화가 있다. 그러므로 이런 침수스트레스 문제에 대응하기 위해서 침수저항성 메커니즘과 관련된 다양한 연구가 수행중이다. 침수저항성이 높은 작물을 생산하기 위해서는 침수 스트레스의 여러 요인을 연구하는 것뿐만 아니라 어떻게 식물이 침수스트레스 요인을 감지하고 대응하는 방법을 이해해야 한다. 따라서 본 논문에서는 침수 스트레스 저항성과 관련된 것으로 보고된 작물의 형태적, 생리적, 유전자적 변화에 대해서 소개하고 이를 통해 작물의 침수저항성 메커니즘에 대한 이해를 돕기 위해 내용을 정리하였다.

The objectives of this study were to analyze the influence of chemical water quality on fish guilds, pollution tolerance and the multi-metric ecological health, based on the Fish Assessment Index (FAI) in the main stream of Mangyeong River between 2009−2016. The quality of water with specific conductivity, TP, and NH4-N got worse dramatically in the down region. During the study, a total of 50 species were collected and the most dominant species was Zacco platypus. Also known as tolerant species, accounted 22.9% of the total abundances, thus indicating a trophic degradation. The downstream region (S5) had the highest number of fish external abnormalities, indicating a degradation of ecological health, based on the fish assemblages. Pearson correlation analysis indicated that relative abundance of tolerant fish species and omnivore fish species had a significant positive correlation (r>0.30, p<0.05) with values of BOD, conductivity and NH4-N. Whereas, the relative abundance of the sensitive species and insectivore species had a significant negative relations (r<−0.30, p<0.001) with the parameters. The mean obtained from the multi-metric fish model, based on the FAI of all sites was 47 (n=40). This indicated a “fair condition” in the ecological health, and the downstream regions (S3−S5) were judged as “bad condition”, indicating an influence of the chemical degradation on the ecological health.

The setting of values on door hinge mounting compensation for door assembly tolerance is a constant quality issue in vehicle production. Generally, heuristic methods are used in satisfying appropriate door gap and level difference, flushness to improve quality. However, these methods are influenced by the engineer's skills and working environment and result an increasement of development costs. In order to solve these problems, the system which suggests hinge mounting compensation value using CAE (Computer Aided Engineering) analysis is proposed in this study. A structural analysis model was constructed to predict the door gap and level difference, flushness through CAE based on CAD (Computer Aided Design) data. The deformations of 6-degrees of freedom which can occur in real vehicle doors was considered using a stiffness model which utilize an analysis model. The analysis model was verified using 3D scanning of real vehicle door hinge deformation. Then, system model which applying the structural analysis model suggested the final adjustment amount of the hinge mounting to obtain the target door gap and the level difference by inputting the measured value. The proposed system was validated using the simulation and showed a reliability in vehicle hinge mounting compensation process. This study suggests the possibility of using the CAE analysis for setting values of hinge mounting compensation in actual vehicle production.

본 연구는 이탈리안 라이그라스 품종별 내한성 및 생육특성의 변화와 그에 따른 생산성 변화요인을 분석하기 위하여 2017년부터 2018년까지 우리나라 중부지역인 충남 천안에서 수행되었다. 이탈리안 라이그라스의 월동율은 품종 간에 차이가 있었으며(p<0.01), 외국품종보다 국내품종이 대부분 월동이 잘되었다. 조생종에서는 코윈어리의 월동율이 68%로 가장 높았고, Florida 80의 월동율이 35%로 가장 낮았으며, 중생종에서는 코윈마스터의 월동율이 70%로 가장 높았고, 외국품종인 Tam 90이 42%로 가장 낮았다(p<0.05). 외국품종 중에서는 Winter-hawk가 57% 월동하여 다른 외국 품종보다 월동율이 높았다. 건물수량은 품종 간 차이가 많았다(p<0.05). 조생종 중에서는 내한성이 강한 국내품종인 그린팜 2호와 코윈어리가 외국품종인 Florida 80보다 건물수량이 약 3~3.5톤/ha 많았다. 중생종 중에서는 내한성이 강한 국내품종인 코윈마스터가 외국품종인 Winter-hawk나 Tam 90보다 건물수량이 약 2톤/ha 많았다(p<0.05). 이탈리안 라이그라스의 내한성은 건물생산성과 고도의 정의상관이 있었다(p<0.01). 우리나라 중부지역에서 이탈리안 라이그라스를 5월 중순에 수확을 완료해야 한다면, 내한성이 강한 국내 육성 조생 또는 중생품종을 선택하여 재배하는 것이 양질 조사료 생산성 향상에 유리할 것으로 사료된다.

Sperm cryopreservation preserves genetic resources for animal breeding and for human patients who suffers from permanent testicular damage. Although the sperm cryopreservation has been used for many years, the addition of cryoprotective agent (CPA) during cryopreservation negatively affects sperm function and quality. Our previous study reported that the addition of CPA reduced bull sperm physiological functions. However, the sperm cells collected from individual bulls presented different sensitivity to the damage induced by CPA. In the present study, we examined if CPA affect sperm cells acquired from individual bulls. Individual bull spermatozoa were divided into two groups based on motility parameters; high CPA-tolerant sperm (HCS) and low CPA-tolerant sperm (LCS). Our results showed that the HCS group presented good physiological function after CPA exposure, whereas the LCS group showed a significant decrease in the sperm function. We also found differentially expressed five proteins between the HCS and LCS groups, which refer to cytosolic 5′-nucleotidase 1B (NT5C1B), fumarate hydratase (FH), F-actin-capping protein subunit beta (CAPZB), voltage-dependent anion-selective channel protein 2 (VDAC2), and cytochrome b-c1 complex subunit 1 (UQCRC1). NT5C1B and FH showed abundant expression in the HCS group, while the expression of CAPZB, VDAC2, and UQCRC1 was relatively lower in the HCS group than in the LCS group. The current results suggest that NT5C1B, FH, CAPZB, VDAC2, and UQCRC1 can be used as potential markers to predict CPA-tolerable spermatozoa. Those markers provide a reliable tool to select animals and breeds with CPA tolerance.

최근 국제적으로 각 나라의 종 보존 (Species conservation) 및 생태계 보호에 대한 개념이 확대되면서 자국의 본토종의 보호 및 외래종의 교란에 대한 다양한 연구가 활발히 진행되고 있으며, 특히 생태계 교란생물종(동물, 식물 등)은 국가에서 지정하여 이들에 대한 퇴치방안을 강구하고 있다. 정부차원에서는 위해 가능성을 내포하고 있는 종을 ‘생태계 위해 외래생물’(자연환경보전법 제 2조 제 18호)로 지정･고시하고 있다. 현재, 우리나라의 인공호 및 하천(하천/강)에서 블루길(Lepomis macrochirus) 등의 외래어종은 광범위하게 분포하며, 엄청난 속도로 확산되고 있는 것으로 알려져 있다. 외래어 종이 무분별하게 수체내로 유입되면서 생태계와 토착생물에 대한 교란이 발생하고 있으며, 이에 대한 관리방안 모색이 현재 이루어지고 있어 각종 지자체 차원에서도 관리방안이 시급히 요구되고 있는 실정이다. 이에 따라 금강 수계 전체 지점에 대해 수온(Water Temperature; T), 수소이온농도(pH), 용존 산소량(Dissolved Oxygen; DO), 생화학적 산소 요구량(Biochemical Oxygen Demand; BOD), 화학적 산소 요구량(Chemical Oxygen Demand, COD), 전기전도도(Electric Conductivity; EC), 총 부유물질량(Total Suspended Solid; TSS), 총 인(Total Phosphorus; TP), 용존 총 인(Total Dissolved Phosphorus; TDP), 인산염 인(PO4-P), 총 질소 (Total Nitrogen; TN), 용존 총 질소(Total Dissolved Nitrogen; TDN), 암모니아성 질소(NH4-N), 질산성 질소(NO3-N), 엽록소-a(Chllorophyll-a; CHL), 총 유기 탄소량(Total Organic Carbon; TOC) 16개 항목을 활용하여 금강 수계의 수질 범위를 분석하였다. 본 연구에서는 금강 수계에 위치하는 모든 지점의 이화학적 수질 특성 범위를 분석한 뒤, 블루길이 존재하는 지점에서의 이화학적 수질 특성을 분석하여 블루길의 내성도 특성 범위를 파악하기 위해 수행하였다.

외래해충은 자연생태계 교란과 농업환경의 파괴를 가져와 궁극적으로 우리 사회에 엄청난 사회적 비용과 경제적 손실을 유발한다. 이러한 외래해충의 국내 정착 시 대상 해충에 대한 효과적인 방제법과 천적 등의 부재로 박멸에 어려움이 있기에 검역의 역할은 매우 중요하다. 최근 붉은불개미와 금지급 과실파리류는 변화하는 기후와 국가 간교역량의 증가에 따라 최근 국내에서 발견되고 있다. 이를 위해 근연관계에 있는 일본열마디개미와 호박꽃과실파리를 대상으로 내한성을 조사하였다. 그 결과 두 종의 곤충은 저온처리(8℃에서 2시간) 후 체내빙결점의 저하가 나타났다. 또한 저온처리 후 –5℃에서 생존율은 호박꽃과실파리가 일본열마디개미 보다 높은 것으로 조사되었다.

Glutathione S-transferase (GST) is a key gene involved in multiple stress tolerance in all living organisms, though it is still to be disclosed the gene function in teff grass [Eragrostis tef (Zucc.)Trotter].The objectives of this study were to clone and molecular characterization of GST gene in teff grass. We characterized GST1 from teff grass (EtGST1), it composed of a 645-bp open reading frame (ORF) that encoded 195 amino acid residue. Further, we transformed EtGST1 in E.coli BL21 (DE3) cells. This recombinant EtGST1 in E.coli BL21(DE3) induced at 37°C temperature. In addition, Growth of cells overexpressing EtGST1 rapidly increased in the presence of polyethylene glycol (5%), heat (46°C), NaCl (0.6%), and arsenic (1 mM) than that of cells harboring an empty vector. These results suggest that EtGST1 would be suitable candidate for improving tolerance in forages and/or grasses species against multiple abiotic stresses.

Jeju ordinary people have a strong feeling of “Existing the Road to Resentment” from fact of the Jeju 4.3 Grand Tragedy. If we look at some kinds of phenomena or events during 2017 and 2018 during era of Moon Jaein Korean government, I would like to tell those symptoms at Korea and Jeju society. 4.3 Grassroots advocates, including victims, survivors’ families, teachers, students, lawyers, artists, filmmakers, local officials, business people, as well as Jeju, Korean, and international scholars, are fashioning next steps for Jeju 4.3 social healing. For instance, in April 2017, eighteen Jeju 4.3 victims asked the Jeju District Court to set aside unlawful 4.3 convictions based on false accusations and resulting in harsh imprisonment. In September 2018, the Jeju court agreed to reopen the survivors’ cases, granting re-trials. These Jeju survivors and their families’ lawsuit reveals the 4.3 Tragedy’s continuing damage and demonstrates the survivors’ courage in still fighting to clear their names and the names of the thousands similarly convicted during Jeju 4.3. In complementary efforts, in July 2017 a Jeju 4.3 delegation submitted to U.S. Congress foreign affairs committee members a signed petition urging U.S. participation in future 4.3 social healing efforts and asking for congressional hearings on the 4.3 Tragedy. And in December 2017, the Jeju people and Korean and international supporters proposed that the Korean government revise the 4.3 Special Act to authorize meaningful reparations for Jeju 4.3 victims and families. Additionally, Jeju justice advocates are in the process of memorializing the 1947 Jeju 3.1 National Liberation Independence Day demonstrations in the nonviolent spirit of the 1919 national liberty demonstrations. In that spirit, the Jeju people are registering the records of 1947 legal cases of 328 people involved in the demonstrations. With the Jeju peace island human rights movement, the justice supporters are also advocating for recognition of the Jeju 4.3 Grand Tragedy under the UNESCO Memory of the World Programme.

This research was conducted in order to examine the effect of comparative price on consumers’ reactions to service failure in the loyalty program. The current study finds that under condition of lower level of persuasion knowledge, compared to single retail price, comparative price would mitigate consumers’ negative emotion in the context of service failure, which in turn improves customers’ tolerance. The authors test this prediction in two experiments. Experiment 1 aimed to provide an initial exploration of hypothesis by employing a between-subjects design. This experiment recruited undergraduate students at Suzhou University to complete an online survey. After reading the scenario of service failure, participants were asked to answer a series of questions about their intension of spreading negative word of mouth (NWOM). A one-way ANOVA on intention of spreading NWOM suggested that the NWOM activity was significantly lower in the group of comparative prices than in the group of single retail price. In experiment 2, the authors measured angry and intension to complain when participants finished reading the scenario of service failure. Furthermore, one week later the authors measured price tactic persuasion knowledge of participants. The bootstrap method (number of bootstrap samples = 5000, level of confidence = .95) was used. The results showed that customers’ angry level mediated the effect of comparative price on intension of complaint. The authors further conducted a mediated moderation analysis with the participants in the high- and low-persuasive-knowledge conditions. The results indicated the indirect effect of the comparative price on intention to complain was mediated by customers’ angry when they have low persuasive knowledge, whereas the mediation effect of customer’s angry level was no longer significant in the higher persuasive-knowledge condition. To summarize, this research suggested that comparative price would exert a marked impact on the tolerance of members of loyalty program in the context of service failure. The present paper adds to the existing comparative price literatures by confirming its impact on consumers’ judgment in the context of service failure even if they are all members of loyalty program and informed with information of discounted size in advance. Given the special meaning of NWOM and complaint, current paper provides implications for marketing practices.

The beneficial effect of silicon (Si) in increasing salt stress tolerance has been observed in many plants, including the cereal crops rice, wheat, and barley. In this experiment, we examined the effect of Si on the survival and growth of torenia (Torenia fournieri L inden ex F oum) ‘ Duchess Blue and White’ cultured in vitro in the presence and absence of salt stress. Previous reports had suggested that torenia exhibited low salt tolerance. Shoot buds isolated from 16-day-old seedlings were cultured on Murashige and Skoog (MS) medium containing 0, 50, or 100 mM NaCl alone or in combination with 1.8 or 3.6 mM Si supplied as K2SiO3. Plant survival rate was significantly reduced by NaCl supplementation compared with the control. The survival rate significantly increased to 100% when 1.8 or 3.6 mM Si was added to the MS medium containing 50 mM NaCl. However, only 31% of plantlets survived when 1.8 mM Si was added to the culture medium containing 100 mM NaCl. Shoot and root lengths significantly decreased with increasing NaCl concentration in the culture medium, whereas addition of NaCl to the MS medium also significantly reduced fresh and dry weights. However, Si supplementation significantly increased fresh and dry weights under 50 mM NaCl, compared with the control. The greatest fresh and dry weights were recorded when shoot buds were cultured on MS medium containing 50 mM NaCl and 3.6 mM Si. The activities of the antioxidant-scavenging enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT), but not peroxidase (POD), were markedly higher in the presence of 50 mM NaCl than the activity of the control. When Si was added to the medium containing 50 mM NaCl, activities of SOD, POD, APX, and CAT decreased as compared with the 50 mM NaCl treatment. Thus, Si-mediated tolerance to NaCl stress was not due to increased activity of antioxidant enzymes. Although Si was not effective in increasing tolerance to high salt concentrations, such as 100 mM NaCl, the results suggested that Si supplementation could effectively enhance tolerance to 50 mM NaCl stress.

Drosophila melanogaster and Drosophila suzukii are the species of the family Drosophilidae. Although these two fruit flies are taxonomically close species, D. suzukii is thought to be evolutionally adapted to the flesh or maturing fruits, whereas D. melanogaster is adapted to more fermented environments. According to the previous studies, several environmental toxins, such as acetic acid, ethanol, methanol and phenylacetate, ect., have been identified from rotten fruit and fermentation procedures. Considering the differences of distinct habitat between two flies, D. melanogaster is hypothesized to exhibit higher tolerance to the chemical toxins than D. suzukii. Therefore, in this study, we compared the tolerance and susceptibility of two fruit flies to three chemicals (acetic acid, ethanol, 2-phenlyethano).