Considering the situation in the early 20th century when the existing urban system centered on urban areas began to change, the biggest factors causing urban structural changes in urban areas are construction of railroad and urban dismantling. The change process of Eupseong, in the microscopic viewpoint, can be understood as a process of change in the course of dismantlement of town's demarcation, improvement of accessibility and urban expansion due to the construction of railroads, process of urban expansion following the crumbling boundaries and structural changes. This study aimed to look at the transformation process of the Eupseong in the early 20th century, focusing on the demolition of the castle and the railway construction from a microscopic point of view of city.

The object of this thesis is to study the Urban Hanoks around Cheongryangri district. Cheongryangri district became one of Seoul’s administrative districts due to the expansion of the urban area. It is located on the east side of Dongdaemun, the development was to deal with the lack of residential houses in the city. This research was to specify the stylistic characteristics of Urban Hanok in Cheongryangri area by analyzing the changes in residential Urban Hanok for to the influence the market. First, Cheongnyangni Urban Hanok shows the changes in the configuration of plan in accordance with change of the purpose and the way household are divided. Second, as the function of Urban Hanok changed there has been changes in cross-section area. Third, Urban Hanoks with different function have different location. Fourth, the functional change in shops does not cause the change in the plan.

본 연구는 항구도시 칭다오가 20세기 전반에 근대도시로 형성되는 과정에 관한 연구이다. 칭다오의 근대도시화 과정은 동아시아 항구도시의 공통점을 보여주며 강대국 등 정치적 지배 세력에 의해 4개 시기로 구분된다. 첫 번째 독일 점령기 동안에는 칭다오의 구도심 영역이 집중적으로 형성되었다. 두 번째 일본 1차 점령기 동안 칭다오의 도심 영역은 북쪽과 동쪽으로 확장되어 기존 도심의 북쪽으로 금융 및 업무시설이 형성되었고 그 북쪽에서 대항에 이르는 지역에는 공업지역이 넓게 형성되었다. 세 번째 북양군벌 및 국민당 통치기 동안 칭다오는 국제적인 상업과 금융, 휴양도시로서 급격하게 확장되었다. 네 번째 일본 2차 점령기 동안 건축 활동은 활발하지 못했으며 이 기간 동안 일본은 중국 대륙 침략의 관문으로서 칭다오를 활용하고자 했다.

Objectives Here, we report the effect of overexpression of ginseng farnesyl diphosphate synthase on the transcription of three key regulatory enzymes involved in triterpene metabolism in hairy root of ginseng and Centella asiatica (L.) Urban. Materials and Methods A four-year-old root of Panax ginseng C.A. Meyer and Centella asiatica (L.) Urban whole plants were obtained from National Institute of Crop Science (Suwon, Korea) and Chonnam National University (Gwangju, Korea), respectively. Agrobacterium rhizogenes R1000 strain was kindly provided by Dr. In (Nongwoo Bio, Yeju, Korea). Results and Discussion The role of farnesyl diphosphate synthase (FPS) in triterpene biosynthesis (Fig. 1) was investigated. A pCAMBIA3101 vector was used to insert a exogenous gene into target plant genome (Fig. 2). After the transformation, we produced Panax ginseng and Centella asiatica hairy roots by introducing the coding region of the gene from Panax ginseng. In these hairy roots, integration of the transgenes into the C. asiatica nuclear genome was confirmed by PCR analysis using PgFPS (P. ginseng FPS) primers and by Southern hybridization using PgFPS-specific probe. FPS specific activity is increased 4-fold compared to controls. In RT-PCR analysis, overexpression of PgFPS in hairy roots was observed (Fig. 3) and two genes, cycloartenol and beta-amyrin synthase, related to triterpene biosynthesis were up-regulated. These results suggest that FPS overexpression might lead to an enhanced biosynthesis of triterpene saponins and phytosterols. However, we did not demonstrate whether or not the introduction of PgFPS gene in Centella asiatica genome directly enhances triterpene saponin production, although our results showed that gene expression related to triterpene saponin biosynthesis were obviously up-regulated. Therefore, additional experiments such as overexpression of FPS gene in triterpene saponin-deficient mutant plants will be required.