Recently,mushrooms have received attention because they are material of nutritious food health-stimulating properties and medicinal effects. For the purpose of application the antihypertensive Pholiota adiposa and S. cerevisiae into development of new functional traditional rice wine, optimal fermentation condition and sensory evaluation of functional traditional rice wines were investigated and further its angiotensin-I converting enzyme(ACE) inhibitory activities were determined. Addition of antihypertensive Pholiota adiposa ASI 24012 into the mash was unaffected to ethanol production. The best acceptability showed in the PS-1 traditional rice wines which was brewed byaddition of 0.1% Pholiota adiposa ASI 24012 fruiting body into mash containing antihypertensive S. cerevisiae and cooked and also showed high ACE inhibitory activity of 78.3%.