본 연구의 목적은 RAW 264.7 세포에 산초 종자 정유 성분의 항염 및 항산화 효과에 대하여 알아보고자 하였다. 산초 종자로부터 정유를 추출하였으며, 추출물의 항산화 및 항염 활성에 대하여 평 가하였다. 추출물의 항산화 활성은 DPPH 라디칼 소거능과 SOD 유사 활성 평가법을 이용하였다. 또한 세포독성 평가를 위해 MTT를 이용하여 세포생존율을 측정하였다. LPS로 염증이 유도된 RAW 264.7 세포에 대하여 산초 정유가 NO생성과 PGE2 생성 정도에 미치는 영향을 측정하였다. 그 결과 산초 종 자 정유는 농도 의존적으로 DPPH 라디칼 소거능과 SOD 유사 활성이 증가하는 것으로 나타났다. 세포 생존율 평가에서는 40 μg/mL-1 이하의 농도에서 98% 이상의 낮은 세포독성을 나타냈으며, 항염 효능 평가에서는 LPS를 단독으로 처리한 양성대조군보다 산초 정유 성분을 처리한 군에서 NO와 PGE2 생성 이 현저하게 감소되는 것을 확인하였다. 이러한 결과는 산초 정유가 염증매개인자를 감소시키고 산화의 활성을 방지할 수 있는 기능성 식물소재로서의 가능성을 나타낸다.

Rutin is one of the major flavonoids found in buckwheat (Fagopyrum esculentum Moench). While rutin is already known to exhibit anti-oxidative, anti-inflammatory, and anti-carcinogenic activities. However, the health beneficial function of rutin metabolites is not well understood. In DPPH radical scavenging assays, the present study found that 3,4-dihydroxyphenyl acetic acid had the highest total anti-oxidant activity, followed by 3,4-dihydroxyphenylacetic acid, rutin, homovanillic acid, and 3-hydroxyphenyl acetic acid. Further, 3,4-dihydroxyphenylacetic acid strongly reduced LPS-induced IL-6 production in RAW 264.7 cells, compared with other metabolites. Therefore, these results suggest that rutin metabolites have potential to be utilized as food ingredients with anti-oxidant and anti-inflammatory activities.

Fraxinus rhynchophylla (Oleaceae), a deciduous tree, is known to have properties that include anti-inflammatory, convergence, febricide, antiblenophthalmia, antidiarrhea, antileukorrhea, and so forth. In addition, it has been used for traditional herbal medicine in East Asian countries, including Korea. In this study, we investigated the antioxidant and anti-inflammatory effects of Fraxinus rhynchophylla ethanol extract (FRE) in lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells with FRE pretreatment. We performed DPPH-assay, Western blot, and Reverse Transcription-Polymerase Chain Reaction (RT-PCR). FRE showed 85% free radical scavenging activity at concentrations of 80 µg/ml. Results of this study also showed that FRE down-regulates Cox-2 and iNOS expression in mRNA and protein level. In conclusion, crude ethanol extract of Fraxinus rhynchophylla exhibited antioxidant and anti-inflammatory activities, and it may potentially provide a valuable source of natural herbal agent to inhibit inflammation.

The purpose of this study was to investigate anti-oxidant effects of loach muscle-derived peptides in vitro and in vivo. Loach muscle peptides were prepared by 4 different methods: boiling (B), enzymatic hydrolysis (E), boiling and enzymatic hydrolysis (BE), alkaline and enzymatic hydrolysis (AE). Two different in vitro analyses, DPPH radical scavenging activity and xanthine-xanthine oxidase-induced superoxide radical scavenging activity, were performed. All the four preparations showed concentration-dependent DPPH radical scavenging activity. However, superoxide radical scavenging activity was found only with AE and E preparations. To evaluate in vivo effects, mice were fed with 10% AE-containing diets for 4 weeks before hepatotoxicity induction with CCl4. In serum glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) levels, total antioxidant levels, and relative hepatic weight ratio, no evidence for anti-oxidant effects was found with AE indicating the absence of anti-oxidant effect in the in vivo mouse experiment. It needs to be clarified why anti-oxidant activity of loach protein hydrolysates was not evident in vivo. Furthermore, these results suggest that in vivo evaluation is crucial in demonstrating anti-oxidant activities.

마늘의 다양한 생리활성 기능은 잘 알려져 있음에도 불구하고 휘발성 황 화합물을 비롯한 마늘의 독특한 향과 맛으로 인해 가공식품 및 건강기능식품으로 활용하는데 있어 제한점이 많다. 따라서 본 연구에서는 대표적인 김치 발효균인 Lactobacillus plantarum KCTC 3104를 이용하여 마늘을 유산 발효시키고, 발효 마늘을 다시 적정 온도로 가열 처리함으로써 발효과정과 열처리가 마늘의 휘발성 황화합물과 항산화 활성에 어떠한 영향을 미치는지 평가하고자 하였다. 본 연구의 결과를 요약하자면 다음과 같다. (i)마늘에 존재하는 주요 휘발성 황 화합물인 allyl methyl sulfide 및 allyl sulfide, diallyl disulfide는 발효과정과 발효와 열처리 병행과정에서 감소하였으며 특히 열처리는 마늘의 자극적 향미성분을 감소시키는데 크게 기여하였다. (ii)무처리한 G와 발효를 한 FG, 그리고 발효와 열처리를 병행한 FG-H 간에 유의적인 peroxyl radical 소거활성은 차이는 없었다. (iii) 발효와 열처리를 병행한 FG-H는 마늘시료 G 에 비하여 hydroxyl raidcal 소거활성이 증가하였으며 이 증가된 hydroxyl radical 소거활성은 발효 및 열처리를 통해 새로이 생성된 물질에 의한 환원력 및 metal chelating 활성 증가에 기인된 것으로 추측된다. 이상의 연구결과는 발효 및 열처리를 통해 마늘의 자극적 향미를 개선할 수 있다는 가능성을 제시할 뿐만 아니라, 발효 및 열처리 마늘은 항산화 활성, 특히 hydroxyl radical 소거활성이 증가된 식품개발에 기초자료로 사용될 수 있을 것으로 생각된다.

The anti-inflammatory effect of PHBV/Collagen (PHCP) was examined in a mouse model of lipopolysaccharide (LPS)-induced skin inflammation. Vascular permeability on the back skin was measured by the local accumulation of Evan’s blue dye after subcutaneous injection of LPS (30 µg site^-1 ). Dye leakage in the skin showed a significant increase at 2 h after injection of LPS. This LPS-induced dye leakage was also completely inhibited by HO-1 inhibitor, ZnPP, and antioxidants, including methyl gallate, trolox, and mannitol. To study the possible mechanisms underlying the in vivo anti-inflammatory effect of PHCP against LPS-induced inflammation, we also examined the effects of PHCP on malondialdehyde (MDA) and glutathione levels in skin tissues and found that pretreatment with PHCP resulted in inhibited MDA elevation and a remarkable reduction of glutathione level. In addition, similar results were obtained after pretreatment with antioxidants, including trolox and mannitol, and HO-1 inhibitor, ZnPP. Histopathologically, an influx of neutrophils into the skin dermis was detected between 24 h and 72 h after LPS injection (30, 100 µg site^-1), compared to control animals after injection of saline. This increase was greater in mice treated with 100 µg of LPS than in those treated with 30 µg of LPS and was significantly suppressed by pretreatment with PHCP, antioxidants, and HO-1 inhibitor. These results collectively suggest that PHCP has an anti-inflammatory effect against LPS-induced inflammation model in vivo and may be a good candidate for the skin tissue engineering biomedical application primarily through manipulation of the redox state.

4종(種)의 상치를 공시작물(供試作物)로 하여 수분(水分) 스트레스에 노출(露出)을 시켰을 때 총(總) 단백질(蛋白質)의 함량(含量)은 Flooding 처리시 그 감소율(減少率)이 더 크게 나타났으며 감소율(減少率)은 JCM이 가장 컸고, DCM이 가장 작았으며 품종간(品種間) 각 처리별(處理別) 차이(差異)가 크게나타났다. 총(總) 지질(脂質)의 함량(含量)은 감소율(減少率)이 CCM이 가장 컸고 JCM, DCM, DJM순(順)으로 감소(減少)하였다. 항산화효소(抗酸化酵素)인 Superoxide Dismutase(SOD)는 Catalase, Ascorbate Peroxidase의 활성도(活性度)는 전체적으로 감소(減少)하였으며 그 감소율(減少率)은 Catalase의 경우 처리별 JCM이 가장 크게 나타났고 DCM, DJM에서는 Flooding 처리시 효소의 활성도 변화가 더 크게 나타났다. Calatase의 활성도(活性度) 변화(變化)가 Ascorbate peroxidase의 활성도(活性度) 보다 감소율(減少率)이 더 크게 나타나 이는 Catalase가 Ascorbate peroxidase 보다 과산화수소(過酸化水素)와 더 직접적(直接的)으로 반응(反應)을 함을 나타낸다. Drought 처리기간별(處理期間別) 효소(酵素)의 활성도(活性度)는 생육시간(生育時間)이 길어질수록 감소(減少)하는 경향(傾向)이었으며 Catalase의 활성도(活性度)가 4일째 이후 가장 급격하게 감소(減少)하였다.

The root extracts of Paeonia lactiflora cv. ‘Red Charm’ has been studied by many groups, however, little attention has been paid to its flower petal. Paeonia is the genus in the Paeoniaceae family. ‘Red Charm’ Paeonia is a soft-stemmed herbaceous peony hybrid of P. officinalis and P. lactiflora. We previously showed the flower petal extract of Red Charm might have anti-oxidant and anti-inflammatory activities, however, it was not clear which components might be involved in this activity. Bioinformatics analysis previously indicated these extracts have potential anti-oxidant materials. One of them is identified as paeoniflorin, which is major component in root extract of Red Charm. In this study, we compared paeoniflorin and oxypaeoniflorin using DPPH assays to measure its anti-oxidant activities. Oxypaeoniflorin showed higher levels of radical scavenging activity, similar to ascorbic acid control, whereas paeoniflorin did not. Furthermore, nitric oxide assay showed they have similar anti-inflammatory effects. Taken together, these results suggest oxypaeoniflorin may play a more important role in the anti-oxidant activity of the flower petal and root extracts of Red Charm, compared to paeoniflorin. Further studies may be able to provide a platform to develop potential dual effects therapeutics for oxidant-mediated and inflammation-mediated disease in the near future.

Coriandrum sativum L., an annual herbaceous plant of Apiaceae family. The present study evaluated the anti-oxidant activities and anti-inflammatory effects of ethanol extracts of C. sativum. The anti-oxidant activities of C. sativum were measured by total contents of polyphenol, flavonoid, DPPH and ABTS radical scavenging and reducing power activity. And anti-inflammatory effects of C. sativum were measured by LPS-induced RAW 264.7 cells. The results showed that the contents of total polyphenol and flavonoid were 76.03 ± 1.36 mg of gallic acid equivalents/g and 182.23 ± 4.32 mg of rutin equivalents/g at concentration 1 mg/ mL of C. sativum. The DPPH radical scavenging activity was found to be 52.8% at 500 μg/mL. The ABTS radical scavenging activity was shown in 58.3% after exposure to 1,000 μg/mL. Reducing power activity was found to be 66.8% at 2,000 μg/mL. The inhibitory effect of NO production was found to be 65% concentration 500 μg/mL. In the generation quantity of inflammatory cytokines such as TNF-α and IL-1β in cell culture medium, the expression levels of inflammatory proteins in cells were showed decrease with the increase of concentration. Therefore, we suggest that the C. sativum should be a potential source of alternative anti-inflammatory drug with good anti-inflammatory effects.

Veronica persica (V. persica) is a perennial plant that is broadly distributed in Europe, Asia and so on. V. persica is used for pain about the lower abdomen and low back. The aim of this study was to investigate the anti-oxidant and antiinflammatory effects of V. persica ethanol extract in LPS-induced RAW 264.7 cells. To evaluate the anti-oxidant activity, the DPPH and ABTS radical scavenging, total polyphenol and flavonoid contents, and reducing power activity were carried out. The DPPH and ABTS radical scavenging activity were evaluated as 72.0% and 73.0% at the concentrations of 200 and 500 μg/mL, respectively. Total polyphenol and flavonoid contents of V. persica extracts were measured as 65.22 mg/g and 43.82 mg/g at the concentration of 1 mg/mL. The reducing power activity measurement showed 53.0% activity at 1 mg/mL. The anti-inflammatory effects of the V. persica extract were evaluated in LPS induced RAW 264.7 cells. In the evaluation of cell viability by proliferation ＆ cytotoxicity assay kit, the cytotoxicity of the extract was not confirmed at 0~800 μg/mL concentration. And the V. persica significantly inhibited NO production in a concentration dependent manner. The inhibition effects of NO in cell medium of V. persica was over 80% at 800 μg/mL. The V. persica also suppressed the expression of iNOS, COX-2, and phosphorylation of NF-κB and IkB-α proteins. These results indicate that the V. persica has anti-oxidant and anti-inflammatory effects by modulating NF-κB signaling pathways and can be used as natural functional materials.

Background : Oplopanax elatus Nakai. is distributed in Korea and China. In this study, we have used high performance liquid chromatography (HPLC) to compare the internal standards contents [uracil, adenosine, protocatechuic acid, syringin (eleutheroside B) and scoparone (6,7-dimethoxycoumarin)], and compared the antioxidant activity. Methods and Results : Samples were prepared two different temperature conditions (90℃ and 100℃). Total phenolic contents and total flavonoid contents were analyzed while gallic acid and quercetin were used as standard. Anti-oxidant activities were measured by determination of DPPH and reducing power assay. HPLC was reported as five standard compounds equivalent using the following linear equation based on the calibration curve. According to the results, the anti-oxidant effects of Korean O. elatus Nakai. stem extracts in 90℃ water showed more activity than that of Chinese in DPPH assay. However, the amount of internal compounds was higher in Chinese O. elatus Nakai.. The anti-oxidant effects of Korean O. elatus Nakai. stem extracts in 90℃ water showed more activity than Korean O. elatus Nakai. stem extracts in 100℃ water in DPPH assay. In this study, we had found that, at over the 100℃ temperature all the anti-oxidant effects of O. elatus Nakai. extracts were reduced. However, all five standard compounds were detected at similar value. Conclusion : These results suggests that Korean O. elatus Nakai. has higher anti-oxidant activities which can be use for bioactivity assay.

Background : Obesity is a type of metabolic diseases caused by unbalanced in take and consumption of calories. 3T3-L1 is differentiated into adipocytes by various hormones and transcription factors and accumulates intracellular lipid. Therefore, it is important to inhibit the adipocyte differentiation precess for effective obesity inhibition. Aster yomena and Aster glehui are medicinal plant of Compositea family that grows widely in Korea. Aster genus plants have been used to treat snakebite wound or bruises in oriental. The aim of this study was comparison of inhibitory effect oxidation and adipocyte differentiation with Arial parts of A. yomena (AY) and A. glehni (AG). Methods and Results : AY and AG were cultivated from Pyeongchang in Korea, 2018. AY and AG were extracted by 70% ethanol (-E) and water (-W) at room temperature. AG-W has higher phenolic content (6.92 ± 0.23) and AG-E has higher flavonoid content (8.22 ± 0.19) than other extracts. AG-E has higher radical scavenging activity on DPPH and ABTS assay (IC50 value; 104.88 ± 10.50 and 30.06 ± 0.27). In cytotoxity assay, all extracts concentrations of lower 100 ㎍/㎖ were nontoxic to the cells and can be applied for the next assay. The anti-adipogenic effect of extracts were determined in 3T3-L1 cell by Oli Red-O (ORO) staining. The lipid diplot stained with Oil red O was dissolved to determine by microplate-reader. AG-W significantly reduced the adipocyte differentiation of 3T3-L1 cells (70.49%) compared with other extracts (AG-E, AY-W and AY-E). Conclusion : Theses results reveal that the water extract of AG has utility as a functional food material for preventing obesity.

조류 소재에 관한 연구는 꾸준히 진행되고 있다. 본 연구에서는 미역, 다시마, 불등가사리 등 해조류를 혼합 한 발효추출물의 항산화, 미백, 보습의 활성 실험을 진행하였다. 또한 유산균으로는 김치유산균 Lactobacillus sakei균주를 이용하였다. 복합해조류 발효추출물의 생리활성 실험을 진행하였으며, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) 라디칼 소거능에서는 양성대조군 보다 높은 저해효능을 보였다. 미백효능 실험에서는 3,4-dihydroxy-L-phenylalanine (L-DOPA), mushroom tyrosinase 실험을 수행하였으며, 발효 전보다 발효 후의 미백활성이 높게 나타나는 것을 확인할 수 있었다. 한편, 해조류 소재의 유효성분인 fucose에 대한 HPLC분석을 수행하였으며, 용매조건에 대한 표준화 방법을 새로이 정립하였다. 이 연구는 복합해조류 추출물이 항산화, 미백 등 화장품 소재로서 가능성을 제안할 수 있다.

The roots of Rosa multiflora Thunberg have been used in traditional oriental medicine as remedies for rheumatic arthralgia and scabies. In this study, the anti-fungal, anti-oxidant, and anti-inflammatory activities of a supercritical extract of Rosa multiflora root were investigated in vitro. To investigate the anti-oxidant and anti-inflammatory effects of the supercritical extract, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, and the inhibition of nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were examined, respectively. In addition, the anti-fungal activities of the extract were assessed. The results showed a concentration-dependent, increase in ABTS radical scavenging activity. The supercritical fluid extracts of Rosa multiflora root exhibited low toxicity to RAW 264.7 cells at 100 μg/mL the highest concentration tested. Cells stimulated with LPS produced more nitric oxide than normal control cells; however, cells treated with the supercritical fluid extract decreased this production in a concentration-dependent manner. Finally, the supercritical fluid extracts showed significant anti-fungal activity. These results suggest that extracts of the roots of Rosa multiflora might be used to develop potent anti-fungal, anti-oxidant, and anti-inflammatory agents, and may be useful as ingredients for related new functional cosmetic materials.

Rubiae radix is root of Runia akane Nakai, it has been used to hemostasis and blood stasis in Korean and China. This study investigated that anti-oxidant and anti-colorectal cancer effect of ERA (ethanol extract of Rubiae radix) and WRA (water extract of Rubiae radix) using RAW 264.7 (murine macrophage from blood) and HCT-116 cells (human colorectal cancer cell line). ERA contained polyphenol (45.77 ± 2.03 ㎎/g) and flavonoid (22.82 ± 1.33 ㎎/g). 500 μM H2O2-induced ROS generation was diminished by 500 ㎍/㎖ ERA treatment in RAW 264.7 cells, but not WRA (125, 250, and 500 ㎍/㎖). Moreover, caspase-3 activity and DNA fragmentation increased by 500 ㎍/㎖ ERA treatment during apoptotic cell death in HCT-116. Results demonstrated that anti-cancer effect of ERA against human colorectal cancer cells is mediated apoptotic cell death and DNA fragmentation through caspase-3 activation. However, further study is required to what active ingredient of ERA are important for anti-oxidant and anti-colorectal cancer effect in vivo.

Peach seeds contain a large amount of phenolic components and exhibit excellent physiological effects in various diseases. We examined the antioxidant effects of stone and seed of three peach cultivars (Miwhang, MH; Kanoiwa hakuto, KH; and Cheonhong, CH) by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing activity of plasma (FRAP) assay, and cupric ion reducing antioxidant capacity (CUPRAC) reduction. The results showed that the stone extracts of CH had higher levels of total phenols and flavonoids than those of the other cultivars do, and the stone extracts of KH and CH have the potential to reduce DPPH, FRAP, and CUPRAC activities. In addition, we found that KH, MH, and CH stone extracts decreased nitric oxide generation in RAW 264.7 and BV2 cells. The total phenol and flavonoid contents had no significant correlation with anti-oxidant activities. On the other hand, the anti-inflammatory activity had a low linear correlation with anti-oxidant activities and total phenol and flavonoid contents. The present results suggest that the correlation between antioxidant and anti-inflammatory effects of stone and seed, and the appropriate combination of the stone and seed extracts could be used as an anti-inflammatory treatment and prevention material, respectively.

In this study, the anti-oxidant, whitening, and anti-wrinkle effects of Castanea crenata inner shell extracts processed by enzyme treatment and pressurized extraction were investigated. The Castanea crenata inner shell was first hydrolyzed using celluclast, viscozyme, or hemicellulase. Then, it was subjected to pressure extraction for different durations (30, 60, and 120 min). The yields of the Castanea crenata inner shell extracts processed by different enzyme treatments followed by pressurized extraction for different times are in the range of 12.42-29.80%. The total polyphenol, flavonoid, and tannin contents of the C30m (celluclast enzyme and autoclave extracts at 30 min) extract were 15.48, 10.82, and 15.82 g/100 g, respectively. The total sugar content of the H120m(hemicellulase enzyme and autoclave extracts at 120 min) extract is 61.07 g/100 g. The 1,1-diphenyl-2-pycrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities of the C30m extract at 1,000 μg/mL are 89.20, and 81.96%, respectively. The superoxide radical scavenging and ferric-reducing antioxidant power of the C30m extract at 1,000 μg/mL are 67.63% and 1,324.79 μM, respectively. Further, the tyrosinase and elastase inhibition activity of the C30m extract at 1,000 μg/mL are 61.32, and 61.06%, respectively. Our results indicate that the Castanea crenata inner shell extracts processed by enzyme treatment followed by pressurized extraction could have beneficial effects on facial skin and they should be considered for use in new functional cosmetics.

The aim of this study is to investigate the antioxidant and intracellular anti-inflammatory efficacy of blueberry leaf extracted with hot water (BLW), 70% ethanol (BLE), and 70% acetone (BLA) in RAW 264.7 macrophages. In order to evaluate the anti-inflammatory effect of blueberry leaf extracts, RAW 264.7 macrophages were stimulated with lipopolysaccharide (LPS) to induce the production of inflammation-related factors, which were measure by Western blotting and real-time PCR methods. i-NOS, COX-2 protein, and mRNA expression showed concentration-dependent decrease. The decreases in the mRNA expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) were concentration-dependent. Further, the antioxidant effects of blueberry leaf on total polyphenol contents, electron donating ability and ABTS+ radical scavenging activity were evaluated. The total polyphenol contents of BLW, BLE, and BLA were 217.04±2.98, 156.72±3.90, and 182.88±3.02 mg TAE/g, respectively, while the electron donating abilities at 1,000 μg/mL of BLW, BLE, and BLA were 81.7, 79.6, and 79.3%, respectively. The ABTS+ radical scavenging activity was fond to be concentration dependent. The nitric oxide (NO) production inhibition activities at 50 μg/mL of BLW, BLE, and BLA were 35.1, 42.4 and 42.7%, respectively. In conclusion, the antioxidant and anti-inflammatory test results indicate that blueberry leaf extracts (BLW, BLE, and BLA) can be used as potential anti-inflammatory agents.

This study was performed to investigate the correlation between anti-oxidant and anti-inflammatory activities in ripe and unripe fruits of three peach cultivars: Miwhang (MH), Kanoiwa hakuto (KH) and Cheonhong (CH). The unripe fruits had higher levels of total phenols and flavonoids contents than those in the ripe fruits of all the three cultivars. The unripe fruits of CH showed the highest levels of total phenols, flavonoids and antioxidant activities among the fruit samples analyzed. Nitric oxide inhibition values in RAW 264.7 cells for the unripe fruits of MH and KH were 30 and 29%, respectively. However, the inhibition was not observed in unripe CH and the ripe fruits of either cultivar. Total phenols and flavonoids contents showed high linear correlations with the anti-oxidant activities whereas the anti-inflammatory activity had low linear correlations with them.

본 연구의 목적은 8가지 한약재(가지, 금은화, 감초, 천궁, 당귀, 황련, 치자, 연교)로 구성된 가지청열소 독음의 항산화 효과와 항염증 효과를 검증하여 효과적인 화장품소재로서의 가능성을 확인하는 것이다. 한약재의 구성과 비율은 동의보감에 수록된 청열소독음(淸熱蘇毒飮) 처방을 변형시켜 사용하였고 추출은 열수와 70% 에 탄올로 하여 동결건조 분말화하였다. 항산화 효능을 확인하기 위해 라디칼 소거능력(DPPH, ABTS+, superoxide), superoxide dismutase (SOD)유사활성능력, 총 폴리페놀 화합물 함량을 조사하였고 항염증 효능을 확 인하기 위해 LPS (lipopolysaccharide)로 염증반응을 유도시킨 RAW264.7 대식세포에서의 nitric oxide (NO)생성 저해력과 western blot 분석을 통한 염증 관련 단백질 inducible nitric oxide synthase (iNOS)와 cyclooxygenase (COX-2)의 발현 저해를 확인하였다. 그 결과, 가지청열소독음은 뛰어난 항산화 효과와 항염 증 효과를 나타내었고 화장품을 위한 효과적인 성분으로 사용 가능할 것으로 사료된다.