Hypertension is characterized by excessive renin-angiotensin system activity, leading to blood vessel constriction. Several synthetic compounds have been developed to inhibit renin and angiotensin-converting enzyme (ACE). These drugs often have adverse side effects, driving the exploration of plant protein-derived peptides as alternative or supplementary treatments. This study assessed the phenolic compound and amino acid content and the antioxidant and antihypertensive activity of 5 South Korean staple crops. Sorghum had the highest phenolic compound content and exhibited the highest antioxidant activity. Millet grains, particularly finger millet (38.86%), showed higher antihypertensive activity than red beans (14.42%) and sorghum (17.16%). Finger millet was found to contain a large proportion of branched-chain, aromatic, and sulfur-containing amino acids, which are associated with ACE inhibition. In particular, cysteine content was positively correlated with ACE inhibition in the crops tested (r=0.696, p<0.01). This study confirmed that the amino acid composition was more correlated with the antihypertensive activity of grains than the phenolic compound content. Finger millet mainly contained amino acids, which have higher ACE inhibitory activity, resulting in the strongest antihypertensive activity. These findings underscore the antihypertensive potential of select crops as plant-based food ingredients, offering insight into their biological functions.

This study investigated the antioxidant activities of wine made with aronia (Aronia melanocarpa). The ethanol concentration of the aronia wine was increased up to 7.8±0.1% on the 8th day of fermentation. Compared to other types of wine, the total amount of organic acids was highest in raspberry wine, followed by grape wine, arona wine, and aronia juice. Because, acetic, oxalic, and succinic acids were not detected in the aronia juice, but were detected in the aronia wine, it was determined that they were produced during alcohol fermentation. The polyphenol content in the aronia juice and wine was higher than in the grape wine and raspberry wine and was twice as much in the aroni wine than in the aronia juice. The flavonoid content in aronia juice and wine was higher than in commercial grape wine and raspberry wine. The DPPH radical scavenging ability was higher than 50% in the aronia wine and juice samples. ABTS radical scavenging activity was higher in aronia juice and wine than in raspberry wine and grape wine. The results of this study suggest that the development of wine with high antioxidant activity is possible if wine is made with aronia.

The objective of this study was to investigate the distribution of functional compounds in perilla leaves of various genetic resources and their antioxidant activities. A comprehensive analysis of functional compounds was conducted for 90 genetic resources, focusing on total polyphenol content (TPC), total flavonoid content (TFC), individual phenolic content (IPC), and lutein. Their antioxidant activities were then analyzed based on their radical scavenging capacity using ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The TPC content exhibited a range of 13.19 to 35.85 mg gallic acid equivalent/g, whereas the TFC content varied from 11.74 to 46.51 mg catechin equivalent/g. Total IPC was detected in a range of 6,310.98 to 40,491.82 μg/g. Lutein was detected at levels between 70.97 and 597.97 μg/g. ABTS and DPPH radical scavenging activities of perilla leaves ranged from 30.39 to 58.58 mg trolox equivalent (TE)/g and from 7.74 to 46.56 mg TE/g, respectively. Furthermore, correlation analysis demonstrated that rosmarinic acid, a phenolic acid, exhibited a significantly positive correlation with antioxidant activity. These findings suggest that various genetic resource of perilla leaves could effectively mediate antioxidant capacity. Results of this study provide valuable information for use of perilla leaves in Korea as functional food materials.

Lyophyllum decastes has been used for culinary purpose. The present study was conducted to evaluate the antioxidant and anti-inflammatory effects from methanol, acetone, and hot water extracts of L. decastes fruiting bodies. The acetone and methanol extracts showed the higher 1,1-diphenyl-2-picryl-hydrazy radical scavenging activities than that of the hot water extract at 0.5–2.0 mg/mL and was comparable to the BHT, the positive control. The ferrous ion chelating effects of the mushroom extracts at 0.5–2.0 mg/mL were significantly higher than that of BHT. The reducing power of acetone extract (2.12) was significantly lower than that of BHT (2.73) at 2.0 mg/ mL. The mushroom extracts also showed inhibitory effects on production of nitric oxide (NO), and expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide-induced murine macrophage cells in a concentration dependent manner. In vivo anti-inflammatory experiment on carrageenan-induced hind-paw edema of rat model, the acetone extract of the mushroom significantly suppressed the carrageenan-induced rat hind paw edema of rats in a dose dependently. The results suggest that the fruiting bodies of Lyophyllum decastes are a good natural resource of antioxidant and anti-inflammation.

To understand antioxidant enzyme response of two contrasting Arundinella hirta ecotypes to drought stress, drought-tolerant Youngduk and drought-sensitive Jinju-1, were comparatively analyzed changes in the enzymatic activities of peroxidase (POD), ascorbate peroxidase (APX), catalase (CAT), superoxide dismutase (SOD), and glutathione reductase (GR). Two ecotypes, drought-tolerant Youngduk and drought-sensitive Jinju-1 were subjected to drought stress by withholding water for 12 days. ROS accumulation level and electrolytic leakage were significantly increased in both A. hirta ecotypes by drought stress treatment but less in Youngduk than Jinju-1. The RWC significantly decreased in both the drought stress-treated ecotypes as compared to control, but less in Youngduk than Jinju-1. Soluble sugar and protein content were increased more in drought stress-treated Youngduk as compared to Jinju-1. The activities of antioxidant enzymes such as SOD, CAT, POD, APX, and GR increased significantly in both the drought stress-treated ecotypes Youngduk and Jinju-1 as compared to control. The increase in antioxidant enzyme activity level was more prominent in drought stress-treated Youngduk as compared to Jinju-1. Taken together, these results suggest that Youngduk was more tolerant to drought stress than Jinju-1, and seem to indicate that tolerance of A. hirta to drought stress is associated with increased activity of antioxidant enzymes.

To increase antioxidant and antibacterial activities of seasoned soy sauce, five kinds of oriental medicinal plant(Scutellaria baicalensis (P1), Coptis japonica makino (P2), Citriunshius pericarpium (P3), Zizyphi spinosi semen (P4), Crataegus pinnatifida (P5)) and four kinds of medicinal mushrooms(Inonotus obliquus (M1), Hericium erinaceus (M2), Phellinus linteus (M3), Lentinula edodes (M4)) were added to seasoned soy sauce. Soluble solid content, pH, salinity, total polyphenol & flavonoid contents were determined. DPPH & ABTS radical scavenging activities, SOD-like activity, and antibacterial activity were analyzed. Experimental sauces showed decreased pH but significant increases of soluble solid content and salinity. Total polyphenol content was 12.76 μg GAE/g in the control. However, M1 and P1 sauce had significantly higher polyphenol contents at 352.14 and 528.25 μg GAE/g, respectively. Total flavonoids content also showed the same pattern. DPPH free radical scavenging activity was the lowest in the control at 15.75%. It was the highest at 81.80% in M1 and 68.88% in P1. ABTS free radical scavenging activity and SOD-like activity showed the same tendencies. They were higher in the experimental groups than in the control. As for the antibacterial activity analyzed by the paper-disc method, the activity increased the most in P1 and P2. In particular, P2 had the strongest antibacterial activity. Its activity against different microorganisms was in the order of Staphylococcus aureus > Bacillus cereus > Escherichia coli > Salmonella typhimurium. In conclusion, these new sauces show increased antioxidative and antioxidant activities. Therefore, they are expected to be used in various ways as a functional soy sauce.

생물환경조절학회지 31권 4호에 게재된 논문의 사사가 잘못 기재되어 있어 바로 잡습니다.

개똥쑥(Artemisia annua L.)은 국화과 (Compositae)에 속하는 일년생 초본으로 예로부터 진 통, 안면마비 등의 신경계 질환과 위염, 호흡기 등의 질병치료 목적으로 이용되는 식물로 알려져 있다. 개똥쑥을 열수 및 70% 에탄올로 추출하여 추출용매에 따른 추출물의 농도별로 항산화 활성 및 항염증 효과에 미치는 영향을 검토하고자 실시하였다. 총 polyphenol의 함량은 개똥쑥 70% 에탄올 추출물에서 열수 추출물에 비하여 유의적으로 높게 나타났으나, 총 flavonoid 함량은 개똥쑥 열수 추출물과 70% 에 탄올 추출물에서 유의적 차이는 보이지 않았다. DPPH radical 소거능력과 ABTS radical 소거능력은은 62.5∼500 μg/mL 농도에서는 비타민 C 처리구에서 가장 높게 나타났고 그 다음으로는 70% 에탄올 추출물, 열수 추출물 순으로 높게 나타났다. 125 ∼ 500 μg/mL에서 NO 생성 억제효과를 측정한 결 과 열수 추출물에서는 500 μg/mL 농도에서 강한 NO 생성 억제를 보이며, 농도의존적으로 NO 생성 억제를 나타났다. 70% 에탄올 추출물에서는 NO 생성 억제 뿐 아니라 염증성 싸이토카인 발현을 현저 히 억제하는 결과를 보이며, 농도의존적인 억제 효과를 나타났다. 개똥쑥은 항산화효과 및 염증성 싸이 토카인의 발현을 현저히 감소시키는 효과를 보이므로 염증성 질환 치료 및 개선에 널리 사용될 수 있으 리라 사료된다.

This study aimed to investigate the biological activities, including polyphenolic content, flavonoid content, and antioxidant activities, of various cultivars of Korean perilla leaves. The results indicated that among nine cultivars (Namcheon dlggae, Saedora, Nulbora, Donggel 1, Donggell 2, Soim, Sangyeop, Somirang, and Saebom) of perilla leaves, the total polyphenolic content (gallic acid equivalent mg/g, GAE) was the highest in “Nulbora,” while it was lowest in Namcheon dlggae. Moreover, flavonoid content in the extracts of nine cultivars leaves was in the range of 132.93~268.50 mg catechin equivalent/g sample. The antioxidant effects of the perilla leaves were determined using two different in vitro bioassays measuring DPPH and ABTS radical-scavenging activities. The results revealed that antioxidant activity was also higher in “Nulbora” compared with other cultivars. Xanthin-oxidase-inhibition activity ranged from 65.65% to 80.58%, with “Nulbora” exhibiting the highest activity, although the difference with other cultivars was not significant. “Nulbora” extracts reduced the expression of pro-inflammatory genes and several cytokines, including IL-6 activation induced by LPS in macrophages in the range of 100–50 μg/mL. These results suggest that extracts from perilla leaves can be used as bioactive and functional materials that could be important in industrial applications in the future.

The purpose of this study was to investigate the antioxidant activities, quality characteristics, and storage stability of perilla oil mayonnaise (PM) with different levels of onion powder (OP) from 0 to 3%. As the amount of OP increased, the L, a, and b values increased, but pH decreased (p<0.05). The addition of OP raised viscosity (p<0.05). The addition of OP to PM proportionally increased the total phenolic content, and antioxidant activities as measured by DPPH and ABTS radical scavenging activity, and FRAP assay. When compared to PM without OP, PM that contained OP had a peroxide value that was almost 1.94 times lower. The emulsion stability was between 97.62 to 94.92% in the PM without OP and PM with OP groups was 99.60% for 12 weeks. This study showed that the inclusion of OP in PM has the potential to inprove its quality, antioxidant activities, and storage stability.

The aim of this study was to evaluate the probiotic properties of lactic acid bacteria (LAB) isolated from kimchi and antioxidant and anti-inflammatory activities of heat-killed LAB strains. Regarding probiotic properties, Lactiplantibacillus plantarum SMF398 and L. plantarum SMF470 isolated from kimchi can survive under artificial gastric condition and adhere strongly to HT-29 cells. The SMF398 and SMF470 strains showed strong antimicrobial activity and co-aggregation against pathogenic bacteria. The heat-killed cells of LAB (1 mg/mL) were prepared by heating at 121℃ for 15 min. MTT assay revealed that the heat-killed cells (1 mg/mL) of SMF398 and SMF470 were not toxic to HT-29 cells. The heat-killed SMF398 and SMF470 showed significantly higher DPPH and ABTS radical scavenging activities as well as β- carotene bleaching inhibitory activity than the heat-killed L. plantarum ATCC14917, a control probiotic strain (p<0.05). In lipopolysaccharide-induced RAW264.7 cells, the heat-killed SMF398 and SMF470 significantly reduced the nitric oxide production by 30.92% and 22.81%, respectively (p<0.05). Furthermore, the heat-killed SMF398 and SMF470 significantly decreased the gene expression levels of tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2 up to 58.02%, 67.70%, 53.44%, 45.90%, respectively (p<0.05). These results suggest that the heat-killed L. plantarum SMF398 and L. plantarum SMF470 might be useful as antioxidant and anti-inflammatory agents.

This study aimed to evaluate the germination characteristics, -glucan and antioxidant contents, and antioxidant activity of naked oat according to germination temperature (20, 25, and 30oC) and time (24, 48, and 72 h). Sprout length was highest at 25oC, and the rotten degree was increased as the germination temperature and time increased. The -glucan content of naked oat increased after 24 h of germination at all temperatures and then decreased by 72 h of germination. Total polyphenol content, flavonoid content, and antioxidant activity increased as the germination temperature and time increased.

In this study, kombucha was prepared by adding 0%, 25%, 50%, 75%, and 100% of Orostachys japonicus as a natural functional source to broaden its usage and obtain valuable data for fermented beverage research. The kombucha’s pH and sugar content decreased during the fermentation period, but its acidity level increased during the same period. Additionally, its L value increased after decreasing, its a value decreased after increasing, its b value continued to increase, and its sugar reduction increased and then decreased. In the sensory characteristic strength evaluation, its color, fermented odor, and sour taste became stronger, but its sweetness became less. As a result of preference evaluation, fragrance and swallowing capability stood the highest in the 25% addition group, color and taste were the highest in the 50% addition group, and overall preference was highest in the 50% addition group. Total flavonoids, total polyphenols, DPPH, ABTS radical scavenging ability, and reducing power increased until the third day of the experiment and decreased afterward. Therefore, the study determined that fermenting kombucha with 50% Orostachys japonicus extract for 9 days would increase its quality characteristics and provide the most palatability.

This study was conducted to determine the proximate compositions, nutritional components, and antioxidant effects of white and brown enoki mushrooms (Flammulina velutipes). The crude protein and carbohydrate contents were higher in the brown than white mushrooms, whereas the moisture, crude ash, crude lipid, and dietary fiber levels were lower. The mineral contents of the white mushroom was higher than levels obtained in the brown mushroom for the detected components (Ca, Cu, K, Mn, Na, and P). The amount of vitamin B3 in the brown mushroom was 1.51 mg/100 g, which was 4.5 times higher than that in the white mushroom. The major fatty acids detected were palmitic acid, linoleic acid, and α-linolenic acid. The total polyphenol and flavonoid contents were highest in 70% ethanol extracts of the white and brown mushrooms, respectively. For the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, a 70% methanol extract of the white enoki mushrooms showed an activity of 76.4% (p<0.05). For the ferric-reducing antioxidant power (FRAP) activity, a 70% methanol extract of the brown enoki mushrooms showed the highest value. Further, the total flavonoid contents were significantly correlated with the DPPH and FRAP activities.

In this study, we analyzed the antifungal activities of five essential oils (clove, rosemary, thyme, basil, and oregano) against three fungi (Penicillium oxalicum, Penicillium commune, and Cladosporium cladosporioides) isolated from farm-type fermented meat products Though their antifungal activities differed for each fungus, thyme had the greatest effect. Notably, C. cladosporioides showed the highest sensitivity to essential oils, and growth inhibitory effects were greater than for the other two strains. Additionally, ABTS, DPPH, and FRAP analysis confirmed that the five essential oils studied had antioxidant activity. ABTS analysis showed that clove (75%) and oregano (75%) oils had the highest antioxidant activities (both 93.7%). DPPH analysis showed that clove (75%) and rosemary (75%) oils had significantly greater antioxidant activities (both 93.8%) than thyme, basil, or oregano oils. FRAP results indicated that clove and basil oils were the strongest reductants. Comprehensive comparative analysis indicated that clove oil had more antioxidant activity than the other four essential oils. Overall, the study shows that the excellent antifungal properties of clove oil could be harnessed to produce safe fermented meat products by preventing rancidity and mold contamination.

파프리카는 최근 카로티노이드, 비타민C, E, 페놀화합물과 같은 다양한 식물 유래 생리활성물질 등의 효능이 보고되면서 영양성이 풍부한 식품으로서 각광받고 있다. 수확 후 파프리 카 과실의 품질은 모양, 과중, 과피의 색상, 꼭지의 형태 등의 외관상 특징에 의해 주로 결정된다. 이러한 점에서 파프리카 비상품과는 미각적 특성과 영양학적 가치가 고려되지 않은 채 상품과의 50－70% 가격을 받거나 폐기된다. 본 연구는 파프 리카 상품과 및 비상품과의 외적 품질, 일반 성분, 무기물, 항 산화능 및 카로티노이드의 조성을 비교하기 위하여 수행되었 다. 상품성에 따른 과실의 외적 품질 및 일반 성분은 평균 과중 을 제외하고 유의한 차이가 없었다. 무기물 함량과 총페놀은 상품과에서 유의하게 높게 나타났다. 항산화능의 경우, DPPH 라디칼 소거활성은 시료 간에 유의적인 차이는 보이지 않았 다. ABTS 라디칼 소거활성은 더욱이 100과 1,000μg·mL-1농 도에서, 환원력은 500과 1,000μg·mL-1에서 상품과가 비상품 과에 비해 높은 활성을 보였으나 그 외 농도에서는 유의적인 차이가 없었다. β-카로틴을 포함한 주요 활성성분이라 예상되 는 카로티노이드의 함량은 상품과와 비상품과에서 각각 29.3 ± 2.6과 31.9 ± 2.9μg·g-1이었으며, 통계적으로 유의한 차이는 없었다. 실험 결과, 파프리카 과실은 외관상 품질에 따른 영양 성, 항산화능 및 카로티노이드 조성 차이가 미미하거나 없는 것으로 확인된 바, 파프리카 비상품과 과실은 건강 가공식품 의 소재로서 상품과 못지 않은 가치를 지닌 것으로 판단된다.

To investigate the functional activity of different citrus fruit peels, antioxidant compounds in 70% ethanol extracts of mandarin, lemon, orange, and grapefruit peel powders were identified, and antioxidant and antibacterial activities were quantitated. Mandarin peel contained the highest content of total phenolic compounds and total flavonoid substances (21.46±0.12 mg GAE/g and 11.57±0.05 mg RE/g, respectively). The total phenolic compound content of the three other citrus fruits was 14.16±0.18-18.44±0.07, and their total flavonoid content was 5.51±0.10-7.46±0.09 mg RE/g. DPPH radical scavenging activity was the highest in lemon peel (87.64±0.21%), and mandarin peel displayed the best antioxidant activity with respective ABTS radical scavenging activity and FRAP measurements of 43.20±0.61% and 78.82±1.06 mM TE/g. Grapefruit peel antimicrobial activity increased with treatment time, and was the most potent among the four tested citrus species, inhibiting Staphylococcus aureus by about 4.05 log cycle. These findings demonstrate that mandarin and grapefruit peel can be used to prevent oxidation, improve food storage capabilities, and potentially preserve food quality.