Effectiveness of some natural antioxidants were investigated by measuring the physico-chemical charcteristics and fatty acid composition during thermal oxidation in palm oil. Tocopherol showed most enhanced thermal oxidation stabilities compared to the other natural antioxidants. AR spice was no good AOM stability and changes of acid value but the other parameters were obtained desirable results. The changes of linoleic aicd content was slightly decreased during thermal oxidation. Addition of rosemary and glycyrriza extract increased the stabilities of oil less than tocopherol and AR spice. Order of antioxdative effects was tocopherol, AR spice and others. There was no significiant difference in stability of rosemary and glycyrriza extract.

This study was done to determine the effect of antioxidants added perilla oil diet on the content of cholesterol, vitamin E, and lipid peroxide in serum and tissue of rats. Four groups of experimental diets, such none added perilla oil diet, ascorbic acid added perilla oil diet, vitamin E added perilla oil diet, EDTA added perilla oil diet were fed ad libitum to the 4 weeks white male rats of Sprague-Dawley strain. The results obtained are summarized as follow: 1) The body weight gain in all experimental diet groups was higher than the control gorup and EDTA added diet group was lower than the other experimental diet group, while food intake in vitamin E added diet group was the highest and vitamin C added diet group was the lowest in the control group. 2) Total cholesterol levels in serum of all experimental diet groups were lower than that of the control group and especially the level of total cholesterol in none added diet group and vitamin C added diet group were significantly lower than that of control group. 3) HDL-cholesterol levels of all experimental diet groups were lower than that of the control group and especially none added diet group was significantly lower than that of control group. 4) The activities of glutamic oxaloacetic transaminase (GOT) in serum of all experimental diet group except EDTA added diet group were higher than that of the control group and especially none added diet group was significanly higher than that of the control group. The activites of glutamic pyruvic transaminase (GPT) in serum of all experimental diet groups except vitamin C added group were higher than that of control group. 5) Vitamin E levels in serum of none added diet group and vitamin C added diet group were lower than that of the control group and vitamin E added diet group and EDTA added diet group were higher than that of the control group. 6) Vitamin E levels in liver of all experimental diet groups were higher than that of control group and especially none added diet group and vitamin E added diet group were significantly higher than that of the control group. 7) Lipid peroxide in serum of all experimental diet group were lower than that of control group and especially EDTA added diet group. 8) Lipid peroxide in liver and spleen of all experimental diet groups were higher than that of the control group and lipid peroxide in kidney of all experimental diet groups except EDTA added diet group were higher than that of the control group. Four these results, as vitamin C, vitamin E and EDTA added diets have an effect to lipid peroxide by antioxidants, it could be suggested that perilla oil diet has required to add antioxidant because it has not sufficient vitamin E for antioxidant and intake and overtake level of perilla oil diet should be studied to go ahead.

To investigate the protective action of various antioxidants on the photooxidation of rice bran oil, it was irradiated with red and visible light in presence and absence antioxidants with or without sensitizer (methylene blue). 1. Rice bran oil with and without sensitizer in chloroform-ethanol (4:1, v/v) media was largely oxidized under visible light irradiation. on the other hand, rice bran oil without sensitizer was hardly oxidized under red light irradiation. 2. Rice bran oil with sensitizer was oxidized much faster than that without. And the absorbance of it irradiated with visible was increased more than that with red light. 3. The effectiveness of antioxidants on the rice bran oil photooxidation was same order as follows: Irradiation with visible light, no addition of sensitizer β-carotene 〉 dl-α-tocopherol 〉 BHT 〉 BHA Irradiation with visible light, addition of sensitizer β-carotene 〉 BHT 〉 dl-αtocopherol 〉 BHA Irradiation with red light, addition of sensitizer β-carotene 〉 BHA 〉 BHT 〉 dl-α-tocopherol From these results, we concluded that rice bran oil was largely oxidized under visible light irradiation, therefore it must be protected from photooxidative deterioration by the addition of antioxidants.

Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.

Background: Sancho (Zanthoxylum schinifolium Siebold and Zucc) oil is used as a traditional medicinal material to treat severs stomach inflammation and as a diuretic. This study was carried out to investigate the effect of addition of antioxidants and blended oil the storage stability and safety of the biomaterial. Methods and Results: The effects of temperature and light on sancho oil were investigated, and the ability of antioxidants in preventing rancidity of the oil was discovered. Under fluorescent light and in darkness, the acidity of the oil was much lower than that under direct sunlight. The addition of antioxidants decreased the acid value of sancho oil; the antioxidant that showed the best results in this regard was 0.5% propolis. The acid value of canola oil, which had the lowest acid value compared with that of other oils, and blended oil, containing 5% canola oil in sancho oil, decreased by 5.5% and 15%, respectively. About one acid value decrease was observed for every 1% increase in blending with canola oil. As the concentration of canola oil increased, the viscosity and the elightness (L valu) of sancho oil increased slightly, while the blueness (b value) decreased. Conclusions: The results of this study may contribute to ensuring food safety during preservation and the industrialization of the presevation of sancho oil.

Background : Mountain ginseng adventitious roots are being produced in large quantities by using plant tissue cultivation techniques to overcome the disadvantages of recent cultivation techniques. In this study, we intend to provide basic data on the development of cosmetic products by measuring the antioxidants of mountain ginseng adventitious roots extracted from each ethanol concentration and the activity of tyrosinase and elastase enzyme inhibition. Methods and Results : After drying, the mountain ginseng adventitious roots, it was crushed to less than 0.5 ㎜. for testing. It was then extracted at 70℃ for 180 minutes, adding 10 times its weight to each ethanol concentration (0, 20, 40, 60 and 80%) and filtered. The extract is depressurised and used in experiments after freeze-drying. The total phenols and flavonoids content measurement showed the most content in 80% ethanol extract. However, Trolox equivalent antioxidant capacity, ferric reducing antioxidant power showed the best ability in 60% ethanol extract. In addition, the active measurements of tyrosinase and elastase enzyme inhibition performed to check skin whitening and wrinkles improvement showed the highest activity in the 80% ethanol extract. Conclusion : As a result, the optimum conditions for the use of natural antioxidants and functional foods were maximized when it is extracted with 60% ethanol. It is also expected to be valuable as a natural cosmetics material when extracted with 80% ethanol as a solvent when combined with the results of enzyme inhibition activity.

The leaves and stems of Dendropanax morbiferus were separated from organic solvents with methanol. The organic solvent fractions were fractionated with dichloromethane, ethyl acetate and butanol according to the systematic fractionation method. Oxidation in the body induces aging, and antioxidant activity has attracted the attention of many people as a preventive component to suppress negative reactions in the body. To investigate the antioxidant activity of Dendropanax morbiferus were subjected to DPPH free radical assay. In addition, acetyl cholinesterase inhibitions were performed for Alzheimer’s disease as an aging neurological disease. As a result, it was confirmed that the antioxidant effect of DPPH was generally good in the antioxidant test. The ethyl acetate fractions of Dendropanax morbiferus stems and leaves were IC50=30 ㎍/㎖. Acetyl cholinesterase inhibition experiments were carried out at a concentration of 250 ㎍/㎖. Dendropanax morbiferus stems fractions showed dichloromethane fraction of 57.68%, which significantly inhibited the activity of acetyl cholinesterase.

Background : Extraction is the first and the most important step in the recovery and purification of bio-active compounds from plant materials. Many important factors such as solvent, solvent composition, solvent to solid ratio, pH, and temperature significantly influence the extraction efficiency of bio-active compounds. Factorial design of a limited set of variables is advantageous when compared to the conventional method which varies a single parameter per trial. Methods and Results : The objective of this study was to screen independent factors, namely, ethanol concentration (60 – 100%), extraction temperature (40 – 80℃), time (6 – 18 hours), and liquid to solid ratio (10 – 50 ㎖/g) on the recovery of the extract yield, antioxidant capacity, phenolic and flavonoid contents from Dendropanax morbifera leaf using factorial design. Total flavonoid content of extract was determined by colorimetric method with aluminum chloride, while antioxidant activity was screened using the DPPH radical scavenging activity, TEAC and FRAP assays. Full factorial design was employed to determine the significant contribution of the above factors towards antioxidant capacity (TEAC, DPPH and FRAP), and flavonoid contents. Among, all the factors examined, ethanol concentration and extraction temperature are very significant (p < 0.0001), in obtaining higher antioxidant activity, total flavonoid contents. Conclusion : Two level full factorial design screening was successfully employed to determine the significant factors, which are ethanol concentration, temperature, time and liquid to solid ratio in contributing to high antioxidant capacity (TEAC, DPPH and FRAP), and flavonoid content determination from Dendropanax morbifera leaf. From the results obtained, ethanol concentration and temperature was very significant (p < 0.0001), in obtaining higher antioxidant activity and flavonoid contents. Further work on optimization using these significant factors are in progress.

‘적진주찰’은 2002년 하계에 종피가 적색 메벼인 ‘적진주벼’와 SR27753(상남밭벼/적진주벼)를 교배하여 국립식량과학원 벼품종육종연구팀이 계통육종법에 의해 선발하면서 주요 병해충 및 미질검정을 병행하였다. 선발된 우량계통에 대해 2007～2008년 2년간 생산력검정시험을 실시한 결과 조생종이고 종피가 적색이면서 찰벼인 SR28796-10-3-1-1 계통을 선발하여 ‘수원524호’로 계통명을 부여하고 2008～2010년 3년간 지역적응시험 실시한 결과 그 우수성이 인정되어 2010년 12월 농촌진흥청 농작물직무육성신품종선정위원회에서 품종으로 선정되었으며 ‘적진주찰’이라 명명하였다. 이 품종은 중부지역 보통기 보비재배에서 출수기가 8월 2일로 조생종이다. 간장은 85 cm 정도이고, 수당입수는 136개로 약간 많은 편이다. 현미 천립중은 19.9 g이다. 현미의 종피 색은 붉은색이며 찰벼이다. 항산화 성분인 폴리페놀 함량은 310 mg /100 g로 ‘적진주벼’ 177 mg/100 g에 비하여 높고, DPPH인 항산화 활성 정도가 456 mg/100 g으로 ‘적진주벼’의 272 mg /100 g에 비하여 높다. 도열병, 흰잎마름병, 바이러스병 및 해충에 약한 편이다. 현미수량은 중부지역 보통기 보비재배의 3년 평균 4.68 MT/ha의 수준인 품종으로 중부평야지가 재배적응 지역이다.

최근 천년초 선인장은 녹차 및 양파와 같은 피토케미칼(phytochemical)에 함유된 페놀성 화합물의 중요한 소재로 보고되고 있다. 본 연구에서는 천년초 열수 및 80% 에탄올 추출물의 총 페놀 및 총 플라보노이드 함량, 항산화 활성(DPPH 라디칼 소거능 및 환원력) 및 항비만 활성을 평가하였다. 총 페놀 함량은 천년초 열수 추출물 및 80% 에탄올 추출물에서 각각 와 mg GAE/g로 나타난 반면, 총 폴라보노이드 함량은 80% 에탄올

Reactive oxygen species(ROS)에 의한 산화적 손상은 냉동보존 과정과 체외 배양과정 중 세포 생존률 감소의 주된 요인 중 하나이며, 특히 줄기세포의 경우 냉동보존 후 쉽게 분화하거나 사멸하는 경향이 있음이 잘 알려져 있다. 따라서 본 연구는 체외 배양된 인간 조혈모 줄기세포의 냉동보존 시 선별된 항산화제를 처리하여 항산화제가 줄기세포의 생존 및 자동분화에 미치는 영향을 조사하고자 하였다. 해동 후 세포의 생존률은 -tocopherol

Wild grapes have been used as traditional medicinal use and alcoholic beverage production in Korea. The objective of this study is to improve antioxidant properties in Sae-muru by far infrared ray (FIR) treatment, with expecting potential benefits of FIR treatment for wild grape products during manufacturing processes. FIR treatment in berries induced increased content of catechin, epicatechin gallate, epigallocatechin gallate, gallic acid, rutin, ellagic acid, and resveratrol, while content of epicatechin and epigallocatechin was decreased. Although FIR treatment resulted either increased or decreased chemical component groups, presenting in HPLC chromatograms, antioxidant activity in Sae-muru extract was significantly increased by the FIR treatment. Our results suggest that FIR treatment should be an efficient process in the production of high content of bioactive molecules in Sae-muru.

This study was conducted to investigate nitrite scavenging and Angiotensin Converting Enzyme (ACE) effects by antioxidant ingredient and the contents of phenolic compounds is isolated and purified from various sweetpotato cultivars. Various sweetpotato cultivars contained 4 phenol compounds including gallic acid, chlorogenic acid, gentisic acid and caffeic acid. The range of total phenol compound contents within cvs. Saengmi, Seonmi, Hongmi and Zami was at 23.0~48.8mg/100g and cv. Zami containing anthocyanins pigment whose total phenol compound content was at 48.8mg/100g was by far higher than the other cultivars. The assay of antioxidant activity using solvent fractionated extraction methods was investigated in various sweetpotato cultivars. Antioxidant activity of ethyl acetate (41.2~86.2%) was much higher than chloroform and butanol. Antioxidant activity from cvs. Sangmi, Seonmi, Hongmi, Shinyulmi, Zami and Sinchunmi was much higher than α-tocopherol well-known as natural antioxidant. Rancidity inhibitory effect about soybean oil of extracts fractionated by ethyl acetate from cvs. Saengmi, Seonmi, Hongmi and Zami was high considerably. Ethyl acetate layer among various solvent fractionated extraction had a good effect on nitrite scavenging rate but not had a effect on AEC inhibitory. Solvent of column chloromatography to isolate antioxidant contents from ethyl acetate layer was even better on 30% (MeOH) : 70% (Water) condition. There are 5 ingredients at HPLC chromatogram and antioxidant activity effect of individual ingredients was slightly better on Rt 11.9 and Rt 16.9 fraction.

어유에 catechin, 로즈마리 추출물 (Antox1, Antox2, Antox3), carotene, 녹차 추출물 (Gtox) 등을 첨가하여 각각의 항산화력을 rancimat을 이용하여 측정하였으며, synergist외의 상승효과를 측정하였다. 사용된 항산화제 6종 중에서 catechin 500ppm을 첨가한 시료의 induction periods time이 4.35 hr로 control의 0.65 hr보다 3.7 hr의 차이를 보이면서 크게

Seedlings of two rice genotyopes, cvs. Ilpumbyeo and Gancheokbyeo, were exposed to 0, 50 and 100 mM NaCl in nutrient solution for nine days. Plants were collected at the interval of 3 days and organic and inorganic solutes in leaves and roots and antioxidative enzyme activity in leaves were determined. Under salinity, the accumulation of soluble sugars occurred considerably in the older leaves of stressed seedlings compared to younger leaves and roots. The endogenous Na+ contents markedly increased at higher NaCl concentration in leaves and roots of seedlings, though it was higher accumulated in roots. Salinity resulted in an excessive proline accumulation in the stressed plants. A more pronounced increase was observed in Gancheokbyeo leaves. SOD activity in Impumbyeo cannot found any remarkable change, whereas, in Gancheokbyeo, its activity was rapidly decreased. CAT and POD activities increased with an increase in NaCl concentration in both genotypes. In sum­mary, the high capacity of rice seedlings to overcome an unfavorable growth condition such salt stress appears to be related to an adequate partition of organic solutes between shoots and roots and to changes in absorption, transport and re-translocation of salts.

In order to investigate low molecular antioxidants synthesized by enhanced UV-B radiation, we used the seedlings of two rice varieties. Woonjangbyeo, UV-tolerant, and Hwajoongbyeo, UV-susceptible, were subjected under supplemental UV-B irradiation. When rice seedlings were irradiated with UV light for short period, biosynthesis of total phenolic compound, ascorbate and glutathione were momently reduced. With an increase of UV-B radiation, however, those were slightly synthesized. The content of lipid peroxides in UV-challenged rice leaves was considerably increased after 12 hrs of UV-B treatment. Lipoxygenase activity under supplemental UV-B radiation was differently responded on rice varieties.

홍화유 저장 중 천연 항산화제의 종류별 항산화 효과를 측정하기 위하여 로즈마리 추출물, 녹차 추출물, isoflavon, Phaffia rhodozyma extract, tocopherol, sesamol 및 삽성 항산화제인 BHA를 홍화유에 첨가하여 6에서 한달간 저장하며 실험하였다. 저장기간중 점도의 변화 및 항산화력은 녹차 추출물>BHA>tocopherol>로즈마리 추출물>isoflavon>sesamol>Phaffia rhodozyma ex