Biolog ECO plates (31 different carbon sources, Biolog Inc., Hayward, USA) were used to discriminate between rhizosphere soil samples from 4- and 9-year alfalfa stands each with three replication. The growth curves for different groups of carbon sources were nearly sigmoidal, but the maximum rate of utilization was faster for amino acids, carbohydrates and polymers t㏊n for amides, miscellaneous and carboxylic acids, and carbon sources utilization efficiency were all higher in 9-year t㏊n in 4-year alfalfa stand.

The study was conducted to investigate the effects of organic matter application on soil microbial activities and diversities in a newly reclaimed soil. Soil chemical properties, population of microbe, microbial biomass, and properties of microbial community were investigated under 4 different treatment (animal manure compost+ green manure, chemical fertilizer, and without fertilizer). The experiment was conducted for 3 years from 2012 to 2014. The most of chemical properties in the animal manure compost+green manure treatment were increased continually compare to chemical fertilizer and without fertilizer. The population of bacteria and fungi were higher in the animal manure compost+green manure treatment, however, there was no difference on actinomyces. Soil microbial biomass C content was higher in the animal manure compost+green manure treatment than in chemical fertilizer and without fertilizer. Biolog examination showed that catabolic diversities of bacterial communities were higher in the treatment of animal manure compost+green manure. It was showed that principle component analysis of the Biolog data differentiated the organic matter amended soils from NPK and control. These results indicated that application of animal manure compost+green manure had a beneficial effect on soil microbial properties.