This study analyzed the effect of time of trot on hematology and blood chemistry values of the Jeju Pony crossbreed horses that are commonly used for riding (14.1±1.4 years old, Gelding). A total of 28 parameters including vital signs as well as stress hormones such as cortisol and lactic acid levels were examined as the time of the trot exercise progressed. Vital signs such as heart rate (38.0→81.0 times/min) and respiratory rate (11.7→35.7 times/min) increased significantly within 30 minutes of exercise. However, difference in the body temperature was not observed before and after exercise. The hematology including white blood cell count (8.03→9.52×103 cells/μL), red blood cell count (5.94×103→7.23–7.32×103 cells/μL), hemoglobin levels (11.82→14.65–14.78 g/dL), and hematocrit levels (25.04→30.27%) significantly increased 30 minutes after the start of the exercise (p<0.05). The blood chemistry value of albumin (3.25→3.47 g/dL) (p<0.05) only showed a significant increase after the exercise. However, the other blood chemistry levels such as, Na+, K+, Ca2+, total CO2, creatine kinase, glucose, blood urea nitrogen, creatinine, aspartate transaminase, total bilirubin, gamma–glutamyl transpeptidase, and total plasma protein did not change. Also, cortisol and lactic acid levels did not show significant difference. The middle-aged Jeju pony crossbreed horses were not stressed by the 30-minute exercise; therefore, it can be concluded that there is no problem regarding the safety of both the rider and the animal.

TPR(체온, 심박수, 호흡수) 및 혈액 검사는 인간뿐만 아니라 동물의 건강상태와 질병 진단에 폭넓게 이용되고 있고, 말에서는 건강상태와 운동능력의 평가에 중요한 분야이다. 국내에 가장 많은 사육두수를 점유하고 있는 한라마(제주산마)에 대한 운동저항성에 대한 조사보고가 없었 다. 본 연구에서는 한라마의 운동 시간에 따른 TPR과 혈액검사를 통하여 운동저항성을 평가하 고자 실시하였다. 시험축은 한라대학교(제주)에서 관리 중인 한라마 5두를 이용하였으며, 각각 운동방법(평보, 속보)와 시간(5분, 10분, 20분)에 따른 TPR과 혈액화학치를 분석하였다. 혈액화 학치는 근육 운동과 관련성이 높은 Creatine kinase(CK), Ca ionaized, Calcium, Phosphorus, Sodium, Potassium, Lactic acid, Cortisol 및 Aldosterone을 분석하였다. 각각 결과는 mean±SD 로 나타냈으며, 통계처리는 t-test를 이용하였고 p<0.05 수준에서 유의성 검정을 하였다. 평보 지속시간(5분, 10분, 20분)에 따른 체온과 심박수는 각각 37.2 – 37.8℃ 및 45.3 – 52.0회/분 로 차이가 없었다. 하지만 호흡수는 운동전이 평균 28.8회/분에서 운동시작 후 33.8회/분 로 유의하 게 증가하였으나(p<0.05), 운동시간 경과에 따른 차이는 없었다(평균 32.5 – 33.8회/분). 속보 운 동시 체온은 운동전이 37.6℃으나 운동시작 후 평균 38.1℃이상, 심박수도 운동전 44.5회/분에서 10분 운동 후 49.3회/분, 호흡수도 운동전 31.0회/분에서 10분 운동 후 38.3회/분으로 유의하게 상승하였다(p<0.05). 한편 혈액화학치는 운동 강도와 지속시간에 따라 으로 CK 302.25 – 372.50, Ca ionaized 1.37 – 1.46, Calcium 12.05 – 12.22, Phosphorus 2.30-2.48, Sodium 100.84 – 137.25, Potassium 3.58 – 4.55, Lactic acid 0.70 – 1.06, Cortisol 6.67 – 7.88 및 Aldosterone 0.68 – 2.05 수준으로 차이가 없었다. 향후 다양한 운동강도에 따른 추가 연구가 필요할 것으로 생각된다.

This study was evaluated individual growth and hematological changes of young starry flounder, using hyperpigmentation marks on the blind side of body after feeding extruded pellet (EP) and moisture pellet (MP) for 45 days. As a result, the fish grew up to 220.1±47.8 g in the EP treatment and 224.7±42.4 g in the MP treatment, so the weight gain was significantly higher in the MP treatment (74.6±17.3%) than in the EP treatment (63.7±16.9%) (P<0.05). Osmolalities of flounder after 45 days culture were similar between the treatments (P>0.05), showing values of 359.8±4.2 mOsm kg-1 in the EP treatment and 358.5±6.2 mOsm kg-1 in the MP treatment. Similarly, other blood chemistry indicators such as sodium, chloride, total protein, albumin, glucose, total cholesterol, BUN, creatinine, ammonia, triglyceride, cortisol, GOT, GPT and hematocrit showed no differences between the treatments. However, the free fatty acid concentration was significantly higher in the MP treatment than in the EP treatment (P<0.05).

Although the majority of surviving pigs cloned by somatic cell nuclear transfer (SCNT) appear to be physiologically normal, there is a general lack of detailed hemato-physiologic studies for the period of early adulthood to substantiate this claim. In the present study, we investigated variation in blood chemistry and endocrinological parameters between mesenchymal stem cells (MSCs) derived from cloned and normal age-matched female and male miniature pigs. Cloned females and males showed normal ranges for complete blood count assessments. Biochemical assessments showed that γ-GGT, ALT and cholesterol levels of male and female clones were significantly (P<0.05 or P<0.01, respectively) higher than that of age-matched control miniature pigs. Variations in insulin and IGF-1 were higher in female clones than in male clones and controls. Thus, although female and male cloned miniature pigs may be physiologically similar to normal animals, or at least within normal ranges, a greater degree of physiological and endocrinological variation was found in cloned pigs. The above variation must be taken into account before considering cloned female or male miniature pigs for various biomedical applications.

In order to study the effects of Aloe vera treatment on blood glucose level and clinical chemistry in diabetic patients, eight diabetic patients were administered orally with 800 mg of Aloe vera three times a day for three months. The high levels of blood and urine glucose in diabetic patients were significantly reduced by administration of Aloe vera. The increased plasma triglyceride concentration was also significantly reduced by Aloe vera treatment. A little amount of urine bilirubin, hematuria, nitrite, urobilinogen, protein and ketone bodies were detected before treatment, but not detected after Aloe vera treatment. But other blood parameters of clinical chemistry values were not affected by Aloe vera treatment. These data suggest that Aloe vera can be effective in the treatment of the diabetic patients,