본 연구는 배 유기재배 과수원에서 그물망과 정향유물을 처리하였을 때 큰검정풍뎅이에 의한 잎 피해를 예방할 수 있는지 확인하고자 수행 되었다. 2017년 6월 하순에 보성과 함평의 배 과수원에서 그물망, 그물망 + 정향유, 무처리구 등 3처리구를 두고 큰검정풍뎅이 밀도와 잎 피해율을 조사하였다. 또한 그물망 설치 위치를 달리하여 과수원 외부, 평덕시설의 상부, 평덕시설의 하부에 그물망을 설치하여 포획된 성충 개체수를 계수하였다. 그 결과 그물망 처리구에서 20.4~34.7%, 그물망 + 정향유 혼합처리구에서 21.1~38.1% 수준으로 무처리구보다 낮은 성충수가 포 획되었다. 또한 그물망 설치위치에 따른 포획 개체수는 수관하부 설치구 대비 과수원 외곽은 10.9~14.3배, 수관 상부는 5.1~9.1배로 더 많이 포획되었다. 처리구별 잎 피해율을 조사 한 결과 두 지역 모두 무처리구와 비교하여 유의적으로 그물망, 그물망 + 정향유 처리구에서 잎 피해율이 낮았다. 따라서 큰검정풍뎅이에 의한 잎 피해를 예방하기 위해 그물망을 외곽에 처리하는 것이 실용적 대안이라고 판단된다.

Thirteen plant essential oils were tested for their repellent activity against the bean bug Riptortus clavatus. Among the tested oils, caraway (100%) and clove bud oil (92%) significantly repelled the bean bugs at a dose of 0.142㎕/cm2 by using a Y-tube olfactometer. GC and GC-MS analyses revealed that the active components responsible for the effective repellency of caraway and clove bud oil were carvone (75%) and limonene (76.9%); eugenol (100%), isoeugenol (54.3%) and β-caryophyllene (60.0%), respectively. Of the different active fractions, eugenol was the most significant one than the other components with reference to repellent activity against the bean bugs. In the GC-EAD, limonene and carvone of caraway oil were responded to the antenna of Riptortus clavatus.

Optimum concentrations of anesthetic clove oil and anesthetic lidocaine-HCl were determined for a species of adult marine medaka, Oryzias dancena, over a range of salinity conditions, and investigated in a transport simulation experiment by analyzing various water and physiological parameters. Research indicated that the higher the concentration of anesthetic at each salinity, the shorter the anesthesia time at each salinity. At each concentration, fish were anesthetized slower at water salinities over 10 ppt (P<0.05). Anesthesia time at 10 ppt was faster than any other salinity. In 10 ppt salinity, the dissolved oxygen (DO) concentrations and respiratory frequencies of the clove-oil-administered groups decreased until 48 hours (P<0.05), whereas the NH4 + and CO2 concentrations increased until 48 hours (P<0.05). In same period, the DO, NH4 +, and CO2 concentrations and respiratory frequencies all decreased as the clove oil concentration increased (P<0.05). The trends in the DO, NH4 +, and CO2 concentrations and respiratory frequencies in the lidocaine-HCl-administered groups were similar to those in the clove-oil-administered groups. In conclusion, clove oil and lidocaine-HCl are effective anesthetics, improving the transportation of the marine medaka. The results from this study will contribute to safe laboratory handling of the marine medaka, which are commonly required by many research studies and experiments.

The optimum concentrations of clove oil as an anesthetic for olive flounder (Paralichthys olivaceus) and the stress response of the fish to clove oil anesthesia were determined over a range of water temperatures, and investigated in a simulated transport experiment using analysis of various water and physiological parameters. While the time for induction of anesthesia decreased significantly as both the concentration of clove oil and water temperature increased, the recovery time increased significantly (P<0.05). The plasma cortisol concentration in fish at each temperature increased significantly up to 12 h following exposure (P<0.05), then decreased to 48 h (P<0.05). The DO dissolved oxygen concentrations, pH values, and the fish respiratory frequencies decreased over 6 h following exposure to clove oil in all experimental groups (P<0.05), whereas the NH4 + and CO2concentrations in all experimental groups increased up to 6 h (P<0.05). The pH values and DO concentrations increased with increasing clove oil concentration (P<0.05) in the 6 h following exposure, and the CO2 and NH4 + concentrations and the respiratory frequencies decreased with increasing clove oil concentration (P<0.05). The results of this experiment suggest that clove oil reduced the metabolic activity of olive flounder, thus reducing NH4 + excretion and O2 consumption. In conclusion, clove oil appears to be a cost-effective and efficient anesthetic that is safe for use and non-toxic to the fish and users. Its use provides the potential for improved transportation of olive flounder.