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        검색결과 822

        1.
        2024.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 국내산 가시오가피의 건강기능식품 소재로서 의 가능성을 확인하기 위해 산지별 채취한 가시오가피의 유효물질 함량 및 면역 증강 효과을 평가하였다. 태백, 철 원, 삼척, 강원도 농업기술원에서 수확한 가시오가피의 지 표성분인 eleutheroside B 및 eleutheroside E의 분석을 수 행하였으며, 면역 증강에 대한 효과를 관찰하기 위하여 MTT 세포독성 평가, NO 생성량과 cytokine 생성량을 측 정하였다. 지표성분 eleutheroside B의 함량은 채취 지역별 로 70% 에탄올 추출물에서는 2.96±0.11-6.24±0.05 mg/g로 태백에서 가장 높은 함량을 나타냈으며, 열수 추출물에서 는 1.11±0.05-2.11±0.03 mg/g로 태백에서 가장 높은 함량 을 나타냈다. Eleutheroside E 함량은 채취 지역별로 70% 에탄올 추출물에서는 4.93±0.20-10.79±0.03 mg/g을 나타냈으 며 철원에서 가장 높은 함량을 나타냈고, 열수 추출물에서 는 1.75±0.14-3.64±0.05 mg/g로 철원과 농업기술원에서 가장 높은 함량을 나타냈다. 또한, eleutheroside B 및 E 함량은 열수 추출물보다 70% 에탄올 추출물에서 더 높은 함량을 나타냈다. 채취 지역별 가시오가피의 70% 에탄올 추출물은 50-200 μg/mL 농도에서, 열수 추출물은 100-500 μg/mL 농도 에서 RAW 264.7 대식세포에 대한 세포독성을 나타내지 않 았으며, 대식세포의 활성화로 방출되는 NO 성성량을 측정 한 결과, 가시오가피 줄기 추출물에서 NO 생성량이 증가하 는 것을 확인하였으며, TNF-α, IL-6, IL-1β을 포함하는 cytokine의 방출을 측정한 결과 유의적인 증가를 나타냈다. 따라서 가시오가피 줄기는 면역 관련 질환의 개선을 위한 건강기능식품 소재로 활용 가능할 것으로 사료된다.
        4,000원
        2.
        2023.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 국내육성 수국 ‘Morning Star’와 ‘Pink Ari’에 있어서 삽목배지 및 삽목시기가 발근에 미치는 영향을 조사하 기 위해 수행하였다. 삽목배지 실험에서 코코피트, 펄라이트, 피트모스 단용과 코코피트+코코칩을 혼용 처리하였으며, 7월 15일에 ‘Morning Star’의 정아에서 삽수를 채취하여 삽목하 였다. 삽목시기 실험은 4월, 6월, 8월에 정아를 채취하여 삽 목하였다. 삽목배지로써 코코피트와 코코칩의 혼용 배지는 발 근에 가장 부정적인 영향을 미쳤다. 다른 배지들은 발근율과 뿌리길이에서 통계적인 차이가 없었으나, 펄라이트와 피트모 스보다 코코피트에서 뿌리수가 더 많았다. 따라서 국내육성 수국 ‘Morning Star’의 삽목은 코코피트를 배지로 사용하는 것이 발근에 가장 효과적인 것으로 나타났다. 삽목시기에 관 한 실험에서, ‘Morning Star’의 발근율은 삽목시기와 상관없 이 83~90%로 통계적인 유의성이 없었으나, 뿌리수와 생체 중은 4월과 8월보다 6월에 삽목했을 때, 가장 증가하였다. 그 리고, 신초의 길이, 엽수 등의 줄기의 생육도 6월에 삽목한 처 리에서 가장 좋았다. ‘Pink Ari’의 삽목시기 실험에서 발근율 은 4월 삽목보다 6월과 8월 삽목 처리에서 높았고, 뿌리수와 뿌리 생체중은 6월 삽목처리에서 각각 61.4개와 0.31g으로 가장 양호하였다. 또한 엽수와 신초의 길이 등 지상부 생육도 전반적으로 4월과 8월 삽목보다 6월 삽목처리에서 가장 좋았 다. 따라서, 국내육성 수국 ‘Morning Star’와 ‘Pink Ari’는 4 월과 8월보다 6월에 삽목하는 것이 발근과 지상부 생육에 효 과적인 것으로 나타났다.
        4,000원
        3.
        2023.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Mulberry fruit is a superior source of polyphenols, especially anthocyanin, and has a long history of use as an edible fruit and traditional medicine. The anthocyanin composition of mulberry fruit from 15 Korean cultivars was analyzed by ultra-performance liquid chromatography diode array detector with quadrupole time of flight/mass spectrometry (UPLC-DAD-QToF/MS) based on a cyanin internal standard. The four glycosides were identified by comparison with authentic standards and published reports. The major anthocyanin was cyanidin 3-O-glucoside (71.7%), followed by cyanidin 3-O-rutinoside (26.6%). The minor components (total of 1.7%) were pelargonidin 3-O-glucoside and pelargonidin 3-O-rutinoside. The total anthocyanin content (mg/100 g, dry weight) of mulberry fruit varied by cultivar and ranged from 471.5±4.0 (Su Hong) to 4,700.2±54.0 (Gwa Sang2). Among the 15 cultivars examined, Gwa Sang2 showed the highest level of cyanidin 3-O-glucoside (3,133.4±32.6), which was 9-fold higher than that of Su Hong (351.5±3.4). In conclusion, anthocyanin profiles, including pelargonidin 3-O-glucoside and pelargonidin 3-O-rutinoside, were reported for the first time from 15 Korean mulberry fruit cultivars. The results will contribute valuable information on pharmaceutical properties, breeding superior mulberry cultivars, and food industries.
        4,000원
        5.
        2023.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        To harvest marketable cucumbers, high quality seedlings must be used. Producing seedlings in the greenhouse during the low radiation period decreases marketability due to insufficient light for growth. Supplemental lighting with artificial light of different quality can be used to improve low light conditions and produce high quality seedlings. Therefore, this study was conducted to select the appropriate supplemental light sources on the growth and seedling quality of grafted cucumber seedlings during the low radiation period. Three cultivars of cucumber were used as scions for grafting; ‘NakWonSeongcheongjang’, ‘Sinsedae’, and ‘Goodmorning baekdadagi’. Figleaf gourd (Cucurbita ficifolia) ‘Heukjong’ was used as the rootstock. The seeds were sown on January 26, 2023, and grafted on February 9, 2023. After graft-taking, cucumbers in plug trays were treated with RB light-emitting diodes (LED, red and blue LED, red:blue = 8:2), W LED (white LED, R:G:B = 5:3:2), and HPS (high-pressure sodium lamp), respectively. Non-treatment was used as the control. Supplemental lighting was applied 2 hours before sunrise and 2 hours after sunset for 19 days. The stem diameter and fresh and dry weights of roots did not differ significantly by supplemental light sources. The plant height and hypocotyl length were decreased in W LED. However, the leaf length, leaf width, leaf area, and fresh and dry weights of shoots were the highest in the RB LED. Seedling qualities such as crop growth rate, net assimilation rate, and compactness were also increased in RB LED and W LED. After transplanting, most of the growth was not significant, but early yield of cucumber was higher in LED than non-treatment. In conclusion, using RB LED, W LED for supplemental light source during low radiation period in grafted cucumber seedlings improved growth, seedling quality, and early yield of cucumber.
        4,000원
        6.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        It is widely known that forage corn is one of the most important crops in the production of silage and green chops during the summer months. In this study, the characteristics and the productivity of Kwangpyeongok, AGR 41, and Nero IT corn cultivars were evaluated in the regions of Jeju and Cheonan, Korea between 2021 and 2022. In each year, different corn cultivars were sown in different parts of the country on April 7 in Jeju and May 3 in Cheonan. The yield of three cultivars, Kwangpyeongok, AGR 41, and Nero IT were measured at the yellow-ripening stage, including the leaves and stems (Stover) and corn grain. Kwangpyeongok stover and total dry matter (DM) yields were slightly higher in Cheonan than in Jeju over the past two years. The quantity of corn cultivated in all cultivated areas was similar. The average yield of AGR 41, corn quantity and total DM were slightly higher in Jeju than in Cheonan. In terms of stover yield, and total dry matter content of the Nero IT cultivar cultivated in Jeju had a slightly higher yield than the cultivar cultivated in Cheonan. Based on this study, all cultivars can show some variations but not too much in terms of growth productivity and distinct characteristics depending on their location. Overall, AGR 41, and Nero IT cultivars in Jeju exhibit slightly higher levels of productivity than the same cultivars in Cheonan.
        4,000원
        7.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 국내에서 재배되고 있는 절화 장미 품종들에 있 어서 바이러스 감염 여부를 검정하기 위해 실시하였다. 절화 장미의 주산지인 경기도 G시의 장미재배 농장에서 8품종을, 경상남도 C시의 장미재배 농장에서 10품종을, 전라남도 H군 의 장미재배 농장에서 14품종을, 전라북도 J군의 장미재배 농 장에서 9품종 등 총 41품종을 대상으로 ArMV, INSV, TMV, ToRSV, TRSV, TSV, TSWV 등 총 7종류의 바이러스에 감염되었는지를 ImmunoStrip® kit를 이용하여 검정하였 다. 경기도 G시의 장미 재배농장의 ‘Bobos’, ‘Pink Army’, ‘15-712’, ‘18-356’은 TRSV에 감염된 것으로 나타났고, 경상 남도 C시의 장미 재배농장의 ‘Haesal’은 TMV, ‘Querencia’ 는 ToRSV에 감염된 것으로 나타났다. 전라남도 H군의 장미 재배농장의 ‘Deep Purple’은 ArMV와 TRSV에, ‘Lady of Shalott’은 TRSV, ‘Mortimer Sackler’는 TMV와 TRSV, ‘Natal Briar’는 ArMV와 INSV에, ‘Sprit of Freedom’은 INSV, ‘Imogen’은 TRSV, ‘Pink Beauty’는 ArMV, TMV, TRSV에 감염된 것으로 조사되었다. 전라북도 J군의 장미 재배농장의 ‘Amor Zen’, ‘Dignity’, ‘Peach Pangpang’, ‘Yellow Pangpang’ 등 4개 품종은 TRSV에 감염된 것으 로 나타났고, 본 연구에서 조사한 총 41개 품종 중 22개 품 종이 바이러스에 감염되었으며, TRSV에 13 품종이, ArMV와 TMV에 3 품종이, INSV에 2 품종이, ToRSV에 1 품종이 감 염되어 TRSV에 가장 많이 감염된 것으로 나타났다.
        4,000원
        8.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In this study, the characteristics and taste components of six different oyster mushroom cultivars (Gonji-7ho, Santari, Baekseon, Chunchu, Suhan, and Heuktari) were analyzed and compared. The Heuktari mushroom pileus had the lowest brightness index (32.8) and remained dark (brightness index: 30.5) even after blanching. The moisture content of the mushrooms was approximately 90%. The salinity and sugar contents were highest in Heuktari (5.7% and 7.1%, respectively). Gonji -7ho had the highest contraction rates, with a length contraction rate of 16.4% and thickness contraction rate of 23.9%. The total amino acid content was highest in Heuktari (537.8 mg/100 g), but the glutamine content contributing to umami taste was highest in Santari (59.4 mg/100 g) and the aspartic acid content was highest in Baekseon (33.1 mg/100 g). Among the 5?-nucleotide components, guanosine monophosphate, which enhances umami taste, was highest in Baekseon (0.7 mg/g). Baekseon was also calculated to have the highest umami taste concentration based on amino acid and nucleic acid contents (12.7 g/100 g). The results of this study serve as valuable basic data on the physicochemical characteristics of oyster mushroom cultivars grown in Korea.
        4,000원
        9.
        2023.09 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study was conducted to evaluate Korean rice cultivars to search any suitable varieties for the northern region of the Korean Peninsula (North Korea). A total of 92 rice varieties which included 74 bred cultivars from South Korea, 13 collections from North Korea and five local cultivars from China, were involved in the evaluating trials for their performance. Stability at three sites inthe northeastern areas of China, which are located in the northern border areas of North Korea, for three years from 2017 to 2019. The ecologically well-adapted rice varieties were selected at each trial site during the first year and employed again as the materials for the subsequent yield trials. A total of six varieties with relatively better performing and stability were selected through the subsequent trials at three sites for three years: Unkwang, Daejin and Taebaek at Dandong region; Hanseol and Taebong at Longjin region; Taebong and Namweon at Hunchun region, respectively. Dandong is located in the southeast parts of Liaoning, which has similar climatic characteristics to the west coastalregion of North Korea. Longjin is located in the middle-east parts of Jilin, which has also similar climatic characteristics to the northern inland region of North Korea. Hunchun is located in the northeastern parts of Jilin, which has presumably similar climatic characteristics to the northern part of the eastern coastal region of North Korea. Unkwang is characterized by its early maturity, with the heading at the 2nd of August, and reveals a yield potential of 7.11 T/ha with resistance to the blast (BL) and the bacterial leaf blade (BLB) diseases. It is predicted to be suitable for the northern area of the Suyang mountain region and the central inland region of North Korea. Daejin has asemi-early maturity heading around the 6th of August and yields of 6.06 T/ha with resistance to BL, BLB, and stripe virus (SV) diseases. Daejin will be adaptable to the northern area of Suyang mountain region. Taebaek is medium maturing variety of Indica/Japonica type. It is heading at the 14thof August with good ripening at maturity, and has yield potential of 6.84 T/ha with resistance to BL, BLB and SV diseases. It will be adaptable to the southern area of Suyang mountain region. Hanseol is early maturing, heading on the 3rd of August, and yields 6.44 T/ha with the short stature and cold tolerance (CT). It will be adaptable to the northern inland and mountainous regions of North Korea. Taebong is semi-early maturingwith the heading at the 6th of August and has a yield potential of 6.96 T/ha with cold tolerane and blast resistance. Taebong was also outstanding in theHunchun region, indicating a more or less wide adaptability in cold areas. It is expected to be adaptable to the northern inland region of North Korea as well as the northern part of the eastern coastal region. Namweon is a medium-maturing variety with aheading date of August 10, and a yield potential of 4.76 T/ha with cold tolerance and resistance to rice neck blast disease. It is expected to be adaptable to the northern part of the eastern coastal region.
        4,500원
        10.
        2023.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study aims to select eggplant cultivars adaptive to the hot temperature period greenhouse climate by water consumption, and growth performance of plants and fruits of different European eggplant cultivars, including ‘Bartok (BA)’, ‘Bowie (BO)’, ‘Black Pearl (BP)’, ‘Ishbilia (I)’, ‘Mabel (M)’, ‘Vestale (VE)’ and ‘Velia (VL)’, in substrate hydroponic cultivation under hot and humid greenhouse conditions. On the 118 DAT, the leaf number and stem dry weight were highest in ‘VL’, followed by ‘M’, and there was no significant difference in leaf dry weight among cultivars. The marketable fruit number per plant was 16.4 for ‘M’, which was higher than other cultivars, and ‘VE’ and ‘VL’ were 8.5 and 8.8, respectively. The weight per fruit was low for ‘M’ at 136 g, and the highest in ‘VE’ and ‘VL’ at 332 and 281 g, respectively. There was no significant difference in fruit production per plant. In this study, ‘M’, which has high water use efficiency and a large number of fruits, and ‘VL’, which required less quantity to water consumption for producing 200 g of fruit and had a high product weight, will have excellent adaptability in the UAE greenhouse condition.
        4,000원
        11.
        2023.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        영남 평야지에서는 추석전 햅쌀 출하를 목적으로 조기재배나 극조기재배가 증가하고 있는 추세이다. 그러나 극조기 벼 수확 이후에 후작물로 적합한 작목이 없어서 휴경을 하는 농가가 많아 적합한 작부체계 개발을 위한 재배법 개발이 필요한 실정이다. 따라서 본 시험은 극조기 벼 수확 후 이모작 작부체계에 적합한 벼 품종 및 재배시기 설정을 위해 수행하였다. 조생종 14개 품종을 4월 7일, 4월 14일, 4월 21일, 4월 28일에 각각 이앙하였을 때, 극조기 벼 이모작을 위한 기준 출수기인 7월 10일 이전에 출수가능한 품종은 백일미, 주남조생, 중모1032, 진옥이었다. 이 품종들의 적산온도로 추정한 적정 이앙시기는 4월 10일에서 4월 20일 사이로, 이 시기에 이앙할 경우 8월 20일 이내에 수확이 가능하여 8월 하순 후작물과의 작기연결성에 유리한 적합 품종으로 판단된다.
        4,600원
        13.
        2023.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        본 연구는 절화용 튤립(Tulipa spp.) 4품종을 대상으로 재 식밀도에 따른 생장과 발육 특성을 알아보고 광이용효율을 분 석하여 우리나라에서 튤립 촉성재배시 필요한 기초자료를 얻 고자 실시되었다. 절화용 튤립 ‘Novi Sun’, ‘Timeless’, ‘Il de France’, ‘Dow Jones’ 4품종을 대상으로 구근용 재배 상자 (가로×세로×높이; 60cm×40cm×20cm)에 상자당 48, 70, 96개의 구근을 정식하여 저온처리 이후 비닐하우스로 운반되 었다. 그 결과, ‘Novi Sun’과 ‘Timeless’는 온실 이식 이후 35 일 만에 수확되었으며 ‘Il de France’와 ‘Dow Jones’는 그보 다 빠른 30일 만에 수확되었다. 모든 품종에서 재식밀도가 증 가할수록 생체중, 줄기 직경, 엽면적이 감소하였다. 정상개화 율은 96bulbs/box 처리에서 특히‘Novi Sun’ 32.7%과 ‘Timeless’ 4.7%로 감소하였으며 이 두 품종에서 줄기와 꽃의 건물중이 크게 감소하는 것으로 나타났다. 광이용 효율과 밀접 한 지표인 엽면적지수는 재식밀도가 높아질수록 증가하였다. 광합성 효율은 ‘Novi Sun’과 ‘Timeless’의 경우 저광도에서 재식밀도에 따라 큰 차이가 없는 것으로 나타났다. 엽면적 감 소와 형태적인 변화가 작은 ‘Il de France’과 ‘Dow Jones’에 서 광합성 효율이 크게 변화하였다. 재식밀도에 따라 다양한 생육 반응은 유사한 경향은 있으나 품종에 따라 광합성 효율과 동화산물의 분배가 크게 달라지는 것으로 나타났다. 튤립 촉성 재배 시 정상적인 꽃의 발달과 생육 확보를 위해서는 충분한 재식 간격을 확보할 수 있는 재식밀도로 정식해야 한다.
        4,000원
        14.
        2022.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The physiological characteristics, growth, and yield of each regional rice variety (‘Odaebyeo’, ‘Saechucheong’, ‘Ilmibyeo’) were investigated depending on the impact of changes in temperature and CO2 concentration. Experiments were conducted with a control group, which reflected atmospheric CO2 concentration and temperature, and treatment groups, in which the CO2 concentration and temperature were increased by 250 ppm and 2.0℃ from those in the control group. The results showed that the increase in CO2 concentration and temperature reduced the growth and yield of the rice ‘Odaebyeo’, but did not substantially change the productivity of the ‘Saechucheong’ and ‘Ilmibyeo’. The increase in CO2 concentration and temperature increased stomatal conductance and rate of transpiration of the ‘Odaebyeo’ variety, thereby decreasing its water use efficiency (WUE). In contrast, the increase in CO2 concentration and temperature increased the photosynthetic rate and WUE of the ‘Saechucheong’ and ‘Ilmibyeo’ varieties. The gradual change in climate is considered to directly affect growth and development of rice and diversely affect the productivity of each variety. Therefore, it is necessary to implement technological development, select regionally optimal rice varieties, develop new rice varieties, as well as conduct long-term monitoring of each rice variety for climate adaptation to counter global warming.
        4,200원
        15.
        2022.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study compares the quality characteristics of Jeung-pyun prepared by supplementing with rice mash of various cultivars. Results showed high contents of crude protein and crude ash in Saeilmi. The highest and lowest amylose contents were obtained in Goami4 and Baekjinju, respectively. Saeilmi had the highest water absorption index (WAI) of rice flour, whereas the highest water-soluble index (WSI) was obtained in Baekokchal. Maximum viscosity, minimum viscosity, and breakdown were high in Baekjinju, and high cooling viscosity and setback levels were determined in Goami4. The sugar content, total free sugar, and pH of the rice mash were highest in Baekjinju. The highest volume of Jeung-pyun was obtained with Saeilmi supplementation, whereas the specific volume was highest in Baekokchal. Evaluation of L, a, and b color values of Jeung-pyun revealed the maximum L value in Saeilmi, a value in Goami4, and b value in Baekjinju. The physical properties of Jeung-pyun were lower in all supplemented groups compared to the control group for hardness, adhesiveness, and chewiness. The lowest chewiness was obtained in Baekokchal-supplemented Jeung-pyun. We conclude that supplementation with different varieties of rice affects the quality characteristics of Jeung-pyun, which are important factors for manufacturing processed foods.
        4,000원
        16.
        2022.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study aimed to investigate the biological activities, including polyphenolic content, flavonoid content, and antioxidant activities, of various cultivars of Korean perilla leaves. The results indicated that among nine cultivars (Namcheon dlggae, Saedora, Nulbora, Donggel 1, Donggell 2, Soim, Sangyeop, Somirang, and Saebom) of perilla leaves, the total polyphenolic content (gallic acid equivalent mg/g, GAE) was the highest in “Nulbora,” while it was lowest in Namcheon dlggae. Moreover, flavonoid content in the extracts of nine cultivars leaves was in the range of 132.93~268.50 mg catechin equivalent/g sample. The antioxidant effects of the perilla leaves were determined using two different in vitro bioassays measuring DPPH and ABTS radical-scavenging activities. The results revealed that antioxidant activity was also higher in “Nulbora” compared with other cultivars. Xanthin-oxidase-inhibition activity ranged from 65.65% to 80.58%, with “Nulbora” exhibiting the highest activity, although the difference with other cultivars was not significant. “Nulbora” extracts reduced the expression of pro-inflammatory genes and several cytokines, including IL-6 activation induced by LPS in macrophages in the range of 100–50 μg/mL. These results suggest that extracts from perilla leaves can be used as bioactive and functional materials that could be important in industrial applications in the future.
        4,200원
        17.
        2022.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In vitro digestibility and protein digestibility corrected amino acid scores (PDCAAS) were investigated to verify the availability of protein in various Rhizopus oligosporus fermented products of domestic soybean (Glycine max L.) cultivars. Danbaegkong (DBK), Daepung (DP), Daewonkong (DWK), Saedanbaek (SDB), Seonyu (SY), and Cheongja4ho (CJ4) were used as raw samples, which were fermented using commercially available Rhizopus oligosporus for 48 h. All cultivars showed increased crude protein content after fermentation. The crude protein content of DBK and SDB was significantly higher than that of the other samples (55.12% in DBK and 54.22% in SDB) (p<0.001). CJ4 had the highest alanine content of 28.88 mg/g (p<0.001), and no significant difference in cysteine content was detected among the cultivars. In most of the fermented samples, the in vitro digestibility was 0.9 or higher, indicating high protein in the fermented samples. However, it is considered that restrictions on digestion are low. In DWK, the amino acid content and PDCAAS, which together indicate protein quality, were 0.917 and 0.855, respectively, confirming that it was the best cultivar to provide the raw material for fermentation. In conclusion Rhizopus oligosporus fermented soybean products can be considered a prospective source of protein with high utility value.
        4,000원
        19.
        2022.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        밀은 세계 3대 주요 작물이지만 우리나라는 대부분 수입에 의존하고 있으며, 자급률이 1%도 도달하지 못한 실정이다. 국내 밀 품종은 40여 종 이상으로 지속적으로 개발되고 있으며, 품종마다 용도와 농업적 특성이 달라 목표 형질에 맞는 적절한 품종을 선택하여 재배하는 것이 중요하다. 품종을 정확하고 빠르게 판별하기 위해선 분자 마커 기술이 필요하다. 분자 마커는 생육 환경과 시기에 영향을 받지 않고 다양한 품종을 신속하고 정확하게 구분할 수 있어 유전적 변이성, 농업 형질 등을 분석하기에 유용하다. 본 연구는 기존에 보고된 국내 밀 32품종에 대한 SCAR (Sequence Characterized Amplified Region) 판별 마커를 재검정하여 재현성이 높은 마커를 선별하였으며, 기존에 검정되지 않은 국내 밀 9품종에 추가 적용하여 비교분석하였다. 15개의 마커 세트 중 6개의 마커가 재현성과 정확도가 높은 것으로 확인되었고, 최종적으로 국내 41품종 중 4, 7, 8, 10, 11, 12번 마커를 이용하여 다홍, 금강, 밀성, 조은, 수강, 한백, 조광, 영광을 판별할 수 있었다. 또한, 4번 마커를 통한 증폭산물의 염기서열 분석을 통해 SNP (Single Nucleotide Polymorphism)를 발굴하여 ‘올밀’에 대한 새로운 품종 판별 마커인 SdHRM1과 SdHRM2를 개발하여 HRM (High Resolution Melts) 분석을 시행하여 육안으로 판단하기 어려운 SNP를 정확하게 판별할 수 있었다. 품종간의 SNP를 활용한 품종 마커 기술은 다양한 농업형질에 적용할 수 있으며, 분자 육종 프로그램에 실질적인 도움이 되는데 큰 기여를 할 것으로 사료된다.
        4,500원
        20.
        2022.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Kale (Brassica oleracea var. acephala) is one of the most frequently consumed leafy vegetables globally, as it contains numerous nutrients; essential amino acids, phenolics, vitamins, and minerals, and is particularly rich in glucosinolates. However, the differences in the biosynthesis of glucosinolates and related gene expression among kale cultivars has been poorly reported. In this study, we investigated glucosinolates profile and content in three different kale cultivars, including green (‘Man-Choo’ and ‘Mat-Jjang’) and red kale (‘Red-Curled’) cultivars grown in a vertical farm, using transcriptomic and metabolomic analyses. The growth and development of the green kale cultivars were higher than those of the red kale cultivar at 6 weeks after cultivation. High-performance liquid chromatography (HPLC) analysis revealed five glucosinolates in the ‘Man-Choo’ cultivar, and four glucosinolates in the ‘Mat-Jjang’ and ‘Red-Curled’ cultivars. Glucobrassicin was the most predominant glucosinolate followed by gluconastrutiin in all the cultivars. In contrast, other glucosinolates were highly dependent to the genotypes. The highest total glucosinolates was found in the ‘Red-Curled’ cultivar, which followed by ‘Man-Choo’ and ‘Mat-Jjang’. Based on transcriptome analysis, eight genes were involved in glucosinolate biosynthesis. The overall results suggest that the glucosinolate content and accumulation patterns differ according to the kale cultivar and differential expression of glucosinolate biosynthetic genes.
        4,200원
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