This study was performed to identify the differentially methylated region (DMR) and to examine the mRNA expression of the imprinted H19 gene in day 35 of SCNT pig fetuses. The fetus and placenta at day 35 of gestation fetuses after natural mating (Control) or of cloned pig by somatic cell nuclear transfer (SCNT) were isolated from a uterus. To investigate the mRNA expression and methylation patterns of H19 gene, tissues from fetal liver and placenta including endometrial and extraembryonic tissues were collected. The mRNA expression was evaluated by real-time PCR and methylation pattern was analyzed by bisulfite sequencing method. Bisulfite analyses demonstrated that the differentially methylated region (DMR) was located between -1694 bp to -1338 bp upstream from translation start site of the H19 gene. H19 DMR (-1694 bp to -1338 bp) exhibits a normal mono allelic methylation pattern, and heavily methylated in sperm, but not in oocyte. In contrast to these finding, the analysis of the endometrium and/or extraembryonic tissues from SCNT embryos revealed a complex methylation pattern. The DNA methylation status of DMR Region In porcine H19 gene upstream was hypo methylated in SCNT tissues but hypermethylated in control tissues. Furthermore, the mRNA expression of H19 gene in liver, endometrium, and extraembryonic tissues was significantly higher in SCNT than those of control (p<0.05). These results suggest that the aberrant mRNA expression and the abnormal methylation pattern of imprinted H19 gene might be closely related to the inadequate fetal development of a cloned fetus, contributing to the low efficiency of genomic reprogramming.

본 연구의 목적은 가로구간 사고특성을 분석하고 주 야간 사고모형을 개발하는데 있다. 이를 위해 청주시 가로구간 24개 도로를 연구대상으로 2007년 사고 자료를 이용한다. 연구의 주요결과는 다음과 같다. 첫째, 가로구간의 주간 사고건수가 야간 사고건수보다 많았지만, 사고율에서는 야간사고율이 높은 것으로 분석되었다. 둘째, 4개의 사고모형이 개발되었으며, 이들은 통계적으로 모두 유의한 것으로 분석되었다. 마지막으로, 모형에 채택된 독립변수를 활용하여 주 야간 사고모형의 차이가 비교 분석되었다.

The bean bug, Riptortus pedestris (=clavatus) Fabricius (Hemiptera: Alydidae) is a serious pest of soybean in many countries. It enters a reproductive diapause in the winter by short day length (＜13.5 h.) in Korea and Japan. The combined effects of temperatures (25℃ and 8℃) and day lengths (10L:14D and 14L:10D) upon the termination of the male"s diapause were studied. The combinations are ① HTLD = 25℃, 14L:10D treatment for 1, 2, 3 weeks and 30 days, ② HTSD = 25℃, 10L:14D treatment for 1, 2, and 3 weeks, ③ LTLD→HTLD = 8℃, 14L:10D treatment for 1, 2, and 3 weeks followed by HTLD treatment, ④ LTSD→HTLD = 8℃, 10L:14D treatment for 1, 2, and 3 weeks followed by HTLD. The amount of aggregation pheromone components secreted was adopted as the criteria for diapause termination in males. Males did not secrete aggregation pheromone under HTSD conditions. However, the males treated with HTLD for more than 21 days secreted significantly higher amounts of aggregation components, (E)-2-hexenyl (Z)-3-hexenoate and (E)-2-hexenyl (E)-2-hexenoate, compared to the control or HTSD treatments. The three components of the aggregation pheromone were secreted by the 14%, 29%, and 100% males treated with HTLD for 7 days, respectively. However more than 83% of the HTLD-treated males secreted all three components by the 30<SUP>th</SUP> day under the treatment. In comparison, the pheromone amounts secreted by the males treated with LTLD→HTLD or LTSD→HTLD were equal or less than those secreted by males with continuous HTLD treatment.

The purpose of this study is to design neckties that are motivated by Christmas symbol images that have been known to public most widely in the basis of Day marketing so as to develop the competitive commodities closed to consumers' emotion in the fashion industry. As a method of this study were to use Adobe Illustrator CS2, which is one of the vector graphic programs, to present the motif design such as Santa Claus, trees, presents and letters among Christmas symbols, and are to apply to neckties by giving a change with striped pattern, all over pattern and one point pattern. The results are as follows; Firstly, Santa Claus image was expressed by color contrast with red and white, which was perceived by red, green and white that are mostly used in Christmas. Secondly, tree images are expressed abstractly with color contrast where red and green are contrasted, and color way change was given for symbol color of Christmas. Third, in the image of gift, the image of share and image of colorfulness were considered for expression by making motifs of three dimensional hexahedron shape. Fourthly, in the image of type, motif was expressed by giving a change in horizontal and vertical writing types.

The purpose of the study was to apply and to expand the six sigma to reduce waiting times for computed tomography (CT) examination which manipulated by the department of radiology. It was preceded by DMAIC (Define, Measure, Analyze, Improve, and Control). In the stage of definition, it wereselected for total 5 critical to quality (CTQ), which were the kindness, the waiting time, the examination explanation, the waiting day and the waiting stand environment, that increased the reserved time of CT examination. In the stage of measurement, the number of examinations and of reservation waiting days performed and resulted in final CTQ(Y) which measured each 1.68 and 1.85 sigma. In the stage of analysis, the examination concentrated on morning time, non-scheduled examination of the day, the delayed time of booking, frequent telephone contacting and equipment malfunction were determined as variable key causes. In the stage of improvement, it were performed with expansion of the examination in the morning time, integration of laboratories that used to in each steps, developing the ability of simultaneous booking schedule for the multiple examinations, developing program of examination request, and the customer management team operations. For the control, the number of examinations and reserved waiting days were measured each 3.14 and 1.13 sigma.

The bean bug, Riptortus clavatus Thunberg (Hemiptera: Alydidae), is a pest of soybeans and tree fruits. It enters reproductive diapause during winter. We studied the effect of different combinations of temperature, day length, and treatment period on the termination of diapause in R. clavatus using adult females collected in October and November 2006. Ovarian development was used to determine diapause termination. The treatments were: ① HTLD, 25℃, 14L:10D treatment for 1, 2, 3 weeks and 30 days, ② HTSD, 25℃, 10L:14D treatment for 1, 2, and 3 weeks, ③ LTLD, 8℃, 14L:10D treatment for 1, 2, and 3 weeks followed by HTLD for 3 weeks, and ④ LTSD, 8℃, 10L:14D treatment for 1, 2, and 3 weeks followed by HTLD for 3 weeks. The HTSD treatments did not affect ovarian development, and resulted in no significant difference in the number of mature eggs in ovaries or the percentage of diapause-terminated females compared to the control females before treatment. The percentage of females that terminated diapause was significantly higher in the HTLD treatment than in the HTSD treatment. The HTLD treatment for more than 14 days increased the percentage of diapause-terminated females, accelerated the development of the ovaries, and increased the number of mature eggs in ovaries. Compared with the HTLD or HTSD treatments, the LTLD or LTSD treatments followed by the HTLD treatment accelerated ovarian development and increased the number of ovipositing females. The pre-LTSD treatment for 1 week was enough to increase the number of eggs oviposited.

본 연구는 공급자(supplier), 중간분배자(retailer/distributor) 그리고 고객(customer)으로 구성된 2 단계 공급사슬을 대상으로 공급자가 수요 증대를 목적으로 중간분배자에게 일정기간 동안 제품대금에 대한 지불 연기를 허용한다는 가정 하에 중간분배자의 최적 재고정책 결정을 위한 재고 모형을 다루었다. 소비성 상품의 경우, 고객의 수요는 일반적으로 상품 진열대에 진열되어있는 상품의 재고량에 많은 영향을 받는 것으로 알려져 있다.

본 연구는 노인 주간보호프로그램에 참여하는 노인들의 경험을 이해하고 활동 참여의 과정을 설명하는 것 을 목적으로 하였다. 연구방법 : 본 연구는 노인 주간보호 참여자들의 경험과 그 과정에 대한 깊은 이해를 얻기 위해 질적 연구방법의 하나인 근거이론 접근법을 이용하였다. 참여자에 대한 심층면담, 참여관찰, 그리고 운영일지 등을 통해 자료를 수집하였고, 이를 지속적 비교법을 통해 분석하였다. 결과 : 지역사회에서 장애를 가진 노인들은 일상적인 활동의 결여와 대인관계의 고립으로 인하여 지속적인 건강 의 악화를 경험하였다. 노인 주간보호는 참여자에게 일상적 활동과 사회적 관계 형성의 기회를 제공하였고 이 러한 경험은 새롭게 일상을 재구성하는 과정으로 설명되었다. 결론 : 주간보호에서 활동참여를 통한 일상의 재구성은 일과와 대인관계의 변화와 관련되었다. 노인 주간보호는 활동참여의 기회를 제공한다는 점에서 건강과 활동참여의 연관성을 주목해온 작업치료사들이 보다 관심을 가 져야할 임상영역이다.

This study was conducted to evaluate the attractiveness of the sex pheromone trap of Cnaphalocrocis medinalis Guenée as a forecasting tool from 2006 to 2008 in Suwon, Gyeong-gi province and Seosan, Chung-nam province, Korea. The sex pheromone lure was composed of Z11-18:Ald (55㎍): Z13-18:Ald (500㎍): Z11-18:OH (120㎍): Z13-18:OH (180㎍): Z13-18:Ac (55㎍) per a rubber septum, which set in a cylindrical cone trap. The trap catches of male moths was counted on a weekly basis and the lures were renewed every two weeks. The flight activity of C. medinalis moths monitored by the sex pheromone trap revealed two distinct peaks a year in both experimental areas from 2006 to 2008. Degree-days accumulation was calculated using lower developmental threshold (13.3℃) and degree-days (386.4DD) since adults had been first recorded. Estimated adult peak of the first generation was almost coincident with that observed by sex pheromone trap except in Suwon in 2008.