This study explored the changes in senescence patterns and vase life of cut roses grown in summer and autumn, aiming to identify the relationship between harvest seasons and flower longevity. We analyzed gene expression profiles associated with lignin, pectin, ethylene, auxin, and sucrose transport to understand the molecular mechanisms underlying senescence symptoms, such as the bent neck, petal abscission, and petal wilting in cut rose flowers. Our results revealed season-dependent occurrences of bent neck and petal abscission, with higher incidence rates in autumn-harvested rose flowers. These increases in bent neck and petal abscission contributed to a shortened vase life for the cut flowers. Gene expression analysis indicated that elevated levels of ethylene biosynthesis genes and reduced expression of lignin, pectin biosynthesis, auxin response factor, and sucrose transport genes accelerated the increased senescence symptoms. Notably, the incidence rates of the bent neck were highly negatively correlated with the transcript levels of key genes involved in lignin and pectin biosynthesis, RhPRXPX and RhGAUT1, in pedicels. These findings contribute to our understanding of the molecular factors influencing the mechanical strength of flower pedicels and provide insights for postharvest strategies to enhance the ornamental value of cut flowers across seasons.

Oral bacterial infections substantially affect the development of various periodontal diseases and oral cancers. However, the molecular mechanisms underlying the association between Fusobacterium nucleatum (F. nucleatum ), a major periodontitis (PT)-associated pathogen, and these diseases require extensive research. Previously, our RNAsequencing analysis identified a few hundred differentially expressed genes in patients with PT and peri-implantitis (PI) than in healthy controls. Thus, in the present study using oral squamous cell carcinoma (OSCC) cells, we aimed to evaluate the effect of F. nucleatum infection on genes that are differentially regulated in patients with PT and PI. Human oral squamous cell carcinoma cell lines OSC-2O, HSC-4, and HN22 were used. These cells were infected with F. nucleatum at a multiplicity of infection of 100 for 3 hours at 37℃ in 5% CO2. Gene expression was then measured using reverse-transcription polymerase chain reaction. Among 18 genes tested, the expression of CSF3, an inflammation-related cytokine, was increased by F. nucleatum infection. Additionally, F. nucleatum infection increased the phosphorylation of AKT, p38 MAPK, and JNK in OSC-20 cells. Treatment with p38 MAPK (SB202190) and JNK (SP600125) inhibitors reduced the enhanced CSF3 expression induced by F. nucleatum infection. Overall, this study demonstrated that F. nucleatum promotes CSF3 expression in OSCC cells through p38 MAPK and JNK signaling pathways, suggesting that p38 MAPK and JNK inhibitors may help treat F. nucleatum-related periodontal diseases by suppressing CSF3 expression.

본 연구는 웹툰을 한국어 교육 분야에 활용하기 위한 기초 작업을 위 해 웹툰의 의성·의태 표현의 종류와 특징을 밝히는 것에 목적이 있다. 이 에 두 편의 인기 웹툰에서 무료제공되는 회차에서 300개의 의성어와 의 태어를 추출하여 살펴보았다. 조사 결과 웹툰에 사용되는 의성·의태 표현 이 사전에 등재된 어휘인 경우도 있지만, 사전에 미등재된 어휘인 경우 도 많은 비중을 차지하고 있었다. 형태적으로 변형이 가해지기도 하고, 다양한 품사들이 의성·의태 표현으로 사용되기도 하였다. 그리고 작가의 판단과 필요에 따라 새로운 어휘들이 웹툰 안에서 의성·의태 표현으로 만들어지기도 하며, 하나의 의성·의태 표현이 2개의 의미로 사용되기도 하였다. 기존의 만화 텍스트에서 나타나는 특징과 유사한 특징을 보이지 만 세부적으로는 더 다양한 유형으로 분화되었다. 이렇게 웹툰의 의성·의 태 표현의 변형, 생성이 자유로운 이유를 웹툰의 특징인 실제성, 상호작 용성, 추측 가능성에서 찾을 수 있었다. 기존의 연구들은 웹툰을 언어교 육에 적용하는데 초점이 맞추어져 있다보니 웹툰 언어의 특징을 분석하 지 못한데 반해 본 연구는 웹툰 언어의 특징을 언어학적 관점에서 다루 었다는 데에 의의가 있다고 할 수 있겠다. 본 연구를 시작으로 웹툰 언 어의 특징들을 형태, 의미, 통사적으로 연구될 수 있다면 한국어교육, 한 국어 통·번역 교육에 적용될 수 있으리라 생각된다.

사대부가 창작의 주체인 문인화는 필묵을 숭상하며 채색이 맑고 담백하다. 민화나 궁정 회화에 비해 정신적 의미의 표현 과 화면의 상징적 의미를 더욱 중요시하고 문인의 사고방식으 로 물상을 정신적 차원으로 끌어올려 화가 개인의 정서와 관념 을 투영한다. 이로써 작품은 정신세계의 물질화 형식으로 존재 한다. 그리하여 문인화는 ‘자기 위안’과 ‘유사성을 추구하지 않 고 운율 생성을 추구하는’ 회화 이념을 형성하였다. 문인화는 어떤 기법의 대명사가 아니라 일종의 관념이자 의 식이다. 필묵과 색채의 계승 면에서 채색-흑백-간결한 필묵-필 묵+색채의 발전과정을 거쳤다. 이는 오채의 아름다움을 보여준 당(唐)대에 기원하여 송대에 발전하였다. 당시 궁정 회화가 주 류적 지위를 차지하고 있었지만, 문인화가는 자신만의 회화의 길을 묵묵히 고수하며 탐색하였다. 북송부터 오대 시기까지 색 채를 벗겨냄으로써 차별화된 흑백산수화가 형성되었다. 원대에 는 복잡한 필치의 준찰(皴擦)을 버리고 깨끗하고 간결하게 화 면을 구성하였다. 대부분 수묵 위주이지만 화가는 ‘묵분오색’, ‘여백’ 등의 형식을 통해 문인화의 독특한 필묵의 언어 및 색채 관계를 형성하였다. 명·청시대에는 고대의 기법을 바탕으로 필 (筆)을 뼈대로, 묵(墨)은 살로 삼고 색으로 정신(神)을 입힘으로 써 필묵과 색채가 서로 돋보이도록 하였다. 본고는 회화 주체의 역할 변화 및 시대적 배경의 관점에서 문인화의 ‘기승전결’을 탐구하고, 문인의 내면 정신과 사고방식 을 결합하여 필묵과 색채 표현 간의 부침을 분석한다. 필묵과 채색으로 화면의 색채를 운용하는 관계에서 출발하여 문인화의 비판적인 색채 계승 및 재창조를 해석하고 문인화의 필묵과 색 채 간 긴밀한 연관성을 정리한다. 그러면 문인화를 더 잘 이해 하고 시대정신에 맞는 새로운 작품을 만들 수 있습니다.

In this study, the expression of genes related to fruit ripening was investigated using realtime PCR. The study aimed to determine the effective conditions of calcium compounds (Calcium citrate, calcium chloride, calcium nitrate, GH-Ca, OS-Ca) and chitosan treatment to extend the shelf-life in the ‘Kumhong’ nectarine and ‘Madoka’ peach fruits. In this study, in the ‘Kumhong’ and ‘Madoka’ fruits, the expression of genes related to cell wall degradation, pectin lyase (PL), polygalacturonase (PG), and pectin methylesterase (PME), was inhibited by calcium, chitosan, or a mixture (Chitosan+CaCl2) treatment. In ‘Madoka’ peach, although the expression of the PG gene was suppressed at pre-harvest Ca and chitosan treatment, the expression of the PL gene was induced at pre- and post-harvest Ca and chitosan treatment compared to the control. The spray of calcium, chitosan itself, or a mixture (Chitosan+ CaCl2) on the trees at the ripening stage and harvested fruits can extend the shelf-life by suppressing the expression of cell wall degrading enzymes genes (PL, PG, PME) in ‘Kumhong’ nectarine and ‘Madoka’ peach tree. These results provide valuable information for the development of technology for the extension of the shelf-life of peach and nectarine fruits.

This study examines the rise of the Body Positive Movement on TikTok and its role as a form of online content activism influencing the fashion design and industry. Through a combination of literature review and case study methodology, the study explores the expression techniques and thematic types of Body Positive Movement on TikTok. Reviews of literature, previous studies, online articles, fashion journals, and relevant search terms on TikTok informed a definition of Body Positive Movement and an analysis of its formation and rise. The research findings confirm the impact TikTok content on Body Positive Movement has on the fashion industry in addressing external factors (i.e., ‘Appearance’, ‘Race’, ‘Aging’, ‘Physical Disability’) and intrinsic factors (i.e., ‘Acceptance of Diversity’, ‘Self-Esteem’, ‘Rejection of Stereotypes’, ‘Appropriate Representation’, ‘Information Provision’). The key external factor , ‘Appearance’, includes subcategories such as ‘Body Shape’, ‘Body Hair’, ‘Skin’, and ‘Facial Features’. TikTok content creators on fashion creatively combine music, emojis, and visual storytelling to exhibit positive self-perception concerning these factors. A significant finding of the study is that short clips predominantly manifesting external factors differentiate into informative or enlightening videos associated with intrinsic factors. The study underscores Body Positive Movement's important influence on the fashion industry from design to presentation.

이 연구는 애니메이션을 사회적 임무와 문화적 임무를 짊어진 종합예 술로 보고, 애니메이션에서의 사운드를 대사, 음악, 효과음, 침묵으로 나 눈다. 침묵은 특별한 소리 형태로 감독의 의도를 담고 있으며, 애니메이 션 작품에서 사상성과 견해를 전달하는 사운드 처리 방식 중 하나다. 이 연구는 일본 애니메이션 감독 미야자키 하야오 감독의 애니메이션 작품 “바람계곡의 나우시카”에서 나타난 11번의 침묵 장면을 분석하고, 침묵 부분과 다양한 영상의 조합이 만드는 지향적 활동을 분석하여 현상학 창 시자 에드문트 후설의 지향성 이론을 적용하여 미야자키 하야오 감독이 침묵 장면을 통해 캐릭터의 정신 활동과 내면 감정을 표현하는 방식과 감독이 구축한 태도와 입장을 이해하는 데 도움을 준다. 이 연구는 나타 난 일정한 규칙을 요약하고 정리하여 감독이 작품의 침묵 장면에서 지향 적 내포를 가지고 있음을 확인하고, 이를 통해 침묵 장면이 시청각 체험 연구의 중요성을 강조한다. 이 연구는 시청각 작품에서의 사운드 처리에 서 침묵을 대담하게 사용하고, 더 많은 가능성을 확장하는 데 기여할 것 이다.

Background: Leydig cells, crucial for testosterone production, express ion channels like ANO1 that influence hormone secretion. This study investigates the expression and role of the Tandem of P domains in a weak inward rectifying K+ channel-related Acid-Sensitive K+-1 (TASK-1) channel in these cells, exploring its impact on testicular function and steroidogenesis. Methods: TASK-1 expression in Leydig cells was confirmed using immunostaining, while RT-PCR and Western Blot (WB) validated its expression in the TM3 Leydig cell line. The effect of a TASK-1 channel blocker on cell viability was assessed through live/dead staining and MTT assays. Additionally, the blocker’s effect on testosterone secretion was evaluated by measuring testosterone levels. Results: Immunohistochemical analysis revealed a predominant presence of TASK- 1, along with c-Kit and ANO-1, in Leydig cells adjacent to seminiferous tubules and also in Sertoli and spermatogenic cells. Expression levels of TASK-1 mRNA and protein were significantly higher in TM3 Leydig cells compared to TM4 Sertoli cells. In addition, blocking TASK-1 in TM3 cells with ML365 induced cell death but did not affect LHinduced testosterone secretion. Conclusions: These findings suggest that TASK-1 in Leydig cells is crucial for their viability and proliferation, highlighting its potential importance in testicular physiology.

Uncoupling protein 1 (UCP1) is a unique mitochondrial membranous protein expressed in brown adipose tissue (BAT) in mammals. While its expression in response to cold temperatures and adipogenic inducers is well-characterized in mammals and human infants, the molecular characterization and expression of UCP1 in fish remain unexplored. To address this gap, we analyzed UCP1 expression in response to adipogenic inducers in a fish cell line, rainbow trout gonadal cells (RTG-2), and compared it with UCP1 expression in three mammalian preadipocytes, 3T3-L1, T37i, and WT1 exposed to the Peroxisome proliferator-activated receptor gamma (PPARγ) agonists, rosiglitazone (Rosi). In mammalian preadipocytes, UCP1 protein was highly expressed by Rosi, with an induction of adipogenesis observed in a time-dependent manner. This suggests that UCP1 plays a significant role in adipogenesis in mammals. However, RTG-2 cells showed no response to adipogenic inducers and exhibited only marginal expressions of UCP1. These results imply that RTG-2 cells may lack crucial responsive mechanisms to adipogenic signals or that the adipogenic response is regulated by other mechanisms. Further studies are needed to confirm these phenomena in fish preadipocytes when an appropriate cell line is established in future research.

UV and O3 are materials used in the water treatment process, and many studies have been reported to remove organic matters, contaminants, and microorganisms. In this study, we were investigated effects of Chirnomidae (Chironomus flaviplumus, Chironomus riparius), which are contamination indicator species to exposure UV and O3 for the survival rate, body color change and gene expression response. The survival rate of C. flaviplumus exposed to UV decreased to about 70% after 24 hours, and C. riparius about 50%. There was no change in the survival rate of C. flaviplumus exposed to O3, and C. riparius decreased to 95% after 10 minutes of exposure, but there was no change during the subsequent exposure time. In addition, UV and O3 exposure to the two species in body color faded in a time-dependent. In the HSP70 gene expression, C. riparius showed an increase in expression after UV exposure compared to the control group, and a significant difference was shown 12 hours after exposure (P<0.05). C. flaviplumus exposed to O3 showed a relatively low expression compared to the control group, and showed a significant difference at 10 minutes and 1 hour after exposure (P<0.05). These results reported the ecotoxicological effects on Chironomidae according to UV and O3 exposure. Therefore, the results of this study can be used as basic data to understand the effects of UV and O3, which are disinfectants used in water treatment plants, on Chirnomidae entering plants. Key words: Chironomus flaviplumus, Chironomus riparius, UV, O3, acute toxicity, survival

현대사회에서 미술교육과 미술작품 제작 현장은 정보와 이미 지를 비롯한 폭발적인 성장 속에서 새롭게 전개되고 있다. 이 러한 사회·문화적 방향성의 모색이 필요하고 새로운 관점의 재정립이 요구되는 상황에서 인간과 교육을 둘러싼 사회에서의 미술교육에 대한 새로운 방법론적 접근이 필요하다. 현재 우리 나라 미술교육현장에서 수채화는 점점 도태되어 간다. 화랑가 의 유화 선호, 재료와 도구 사용에 대한 인식, 기법의 난이도 등으로 인해 다른 장르의 그림보다 소외되고 있다. 그 이유 중 가장 큰 문제점은 기초과정을 넘어선 작품 만들기 과정에서 드 러나는데 그것이 바로 보조재료 사용에 대한 미숙함이다. 수채화 제작은 기름을 사용하는 유화와는 달리 물을 사용함으 로 예술의 표현형식과 감상 효과에서 동양인의 체취에 더 친근 감을 주는 매체이다. 수채화 물감을 이용한 작업은 그 자체로 하나의 도전이라고 할 수 있을 정도로 어렵지만, 보조재료를 이 용한 기법을 활용한다면 더 수준 높은 작품성을 발휘할 수 있을 것이다. 수채화에서 중요한 점은 수채화 물감이 투명체 매체라 는 것인데 미세한 입자로 이루어져 있어 어두운색 위에 밝은색 을 덧칠할 수 없다는 것 때문에 작품제작 시 상당한 난관에 부 딪혀 쉽게 작업을 포기하게 되는 것이 현실이다. 본 연구에서는 그런 점을 개선하기 위해 현대미술에서 수채 화 채색작업을 중심으로 <마스킹 액(Masking fluid)> 보조재 료의 활용방안을 제시하고자 한다. 마스킹액을 이용한 여러 가 지 수채화 채색방법과 보조재료의 활용법을 제안함으로써 현장 에서 갖는 문제점에 대해 논의하고, 더욱 질 높은 미술교육과 작품제작에 이바지할 방안을 새롭게 모색하고자 한다.

This study was conducted to evaluate in vitro antioxidant activity of goat meat hot water extracts and the changes in apoptosis-related protein expression levels in the cancer cells treated with these extracts. Goat meat hot water extracts were prepared using different cuts of goat meat, including foreleg, hindleg, loin, and rib. Among these extracts, the foreleg and hindleg extracts displayed higher (P<0.05) ABTS radical scavenging activity than the other two extracts. Protein expression levels of BAX, p53, and p21 were not different in the cells treated with the extracts from different cuts, regardless of the cell type. Only p53 expression in HT-29 cells was elevated (P<0.05) after loin extract treatment. These results suggest that antioxidant activity and apoptosis-related effects of goat meat hot water extract varied with cut of meat under in vitro conditions. Because all data was obtained from the in vitro experiment, the ability to generalize conclusions is limited. Additional in vivo studies are necessary.

본 연구에서는 flavonoid apigenin과 baicalein의 세포 실험으로 콜라겐 회복효과를 확인하고 전사체 비교 분석을 통하여 collagen 회복 효과를 뒷받침할 수 있는 결과를 제안하고자 한다. 연구 결과 apigenin과 baicalein은 HS68 세포주에서 UV에 의해 손상된 I형 collagen 회복에 효과를 보였으며, 두 물질 모두에서 ECM 관련 유전자들의 발현 변화를 확인하였다. ECM을 조절하는 공통적인 메커니즘을 보일 뿐 아니라 각각 다른 범주의 유전자들을 변화시키는 결과도 보여서, 세포에 다양한 영향을 미치는 것으로 예측할 수 있었다.

Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer, and it has been steadily increasing in worldwide. Pituitary tumor transforming gene 1 (PTTG1) has been known as oncogene in a verity of cancers. Nevertheless, the expression and role of PTTG1 in OSCC progression remains largely unexplored. In this study, clinical datasets were analyzed to assess the genetic impact of PTTG1 on OSCC progression and to identify its functional roles in OSCC cell lines. We analyzed the expression of PTTG1 in head and neck squamous cell carcinoma (HNSC) and OSCC using databases form the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO). To investigate the effect of PTTG1 on proliferation and migration abilities in OSCC cell lines, following the knockdown of PTTG1 in HSC-2 and SCC-9 cell lines, we analyzed the proliferation and metastatic abilities of OSCC cells using EdU and Boyden chamber assays. Our database analysis revealed that PTTG1 was significantly overexpressed in tumor tissues compared to normal tissues. Moreover, its expression correlated with clinical parameters of OSCC. In vitro experiments demonstrated that depletion of PTTG1 suppressed the ability of cell proliferation and migration in both HSC-2 and SCC-9 cell lines. In conclusion, our study suggests that PTTG1 may act as an oncogene in OSCC. These findings provide new insights into the mechanisms and clinical implications of PTTG1 expression in OSCC patients.

The honey bee, Apis mellifera, has a defense system, including detoxification, antioxidation, and immunity pathways, against external stimulation such as chemicals, stress, and pathogens. However, pesticides, particularly neonicotinoids and butenolids, have been recently reported to alter physiological changes in honey bee. In this study, we investigated the expression levels of eight genes categorized into detoxification (CYPQ3), antioxidation (CAT and SOD2), and immune system (Abaecin, Apidaecin, Defensin1, Defensin2, and Hymenoptaecin), in five tissues (Head, Thorax, Gut, Fat body, and Carcass) of honey bee treated with three pesticides (Acetamiprid, Imidacloprid, and Flupyradifurone) using quantitative real-time PCR. Gene expression patterns was varied depending on the type of pesticides and tissues. However, among eight genes, the expression levels of CYPQ3 was notably induced, but those of AMPs were generally reduced by all pesticides tested in this study in five tissues. These suggest that CYPQ3-mediated detoxification pathway is induced, but AMP-mediated immune system might be disrupted when honey bee is exposed to neonicotinoids and butenolid.

The adult of honey bee, Apis mellifera, performs an age-dependent division of labor with nurse bees and foragers. Foragers fly outside the hive to collect pollen and nectar, while nurses feed and care for the larvae and queen inside the hive. Foragers are considered to be frequently exposed to agrochemicals, although nurses, stayed inside the hive, are potentially exposed to pesticides through application of miticides and pesticidecontaminated food provided by forager. Therefore, physiological effects of pesticides to nurses should be elucidated to understand the adverse effects of the chemicals on entire honey bee colony. In this study, we investigated the expression changes of the genes associated with labor division (task genes) and the nursing behavior of nurse bees fed four pesticides: acetamiprid (ACE), carbaryl (CB), imidacloprid (IMI), and fenitrothion (FEN). When nurses were exposed to ACE, IMI, and FEN, expression levels of task genes were up- and down-regulated, and their nursing behaviors were also suppressed and enhanced, respectively. CB did not alter the gene expression levels, however increased nursing behavior. These suggest the potential of pesticide that breaks the balance of labor distribution in honey bee colony.

In Korea, the Asian honey bee (Apis cerana) and the European honey bee (Apis mellifera) (Hymenoptera: Apidae) are the two most common honey bee species. These two closely related species are known to have different sensitivity levels to various insecticides due to millennia of exposure to different pests and pesticides. It is reported that A. cerana is known to be more sensitive to several insecticides, such as amitraz, fenitrothion, and fipronil, than A. melllifera. Multiple studies investigated toxicological responses and related CYPome in A. mellifera, but little is known in A. cerana. The goal of this study is to elucidate the underlying mechanism of different toxicological responses between two bee species, with an emphasis on cytochrome P450 (P450), a significant enzyme involved in metabolic activities. The differences in basal P450 expression patterns were investigated by comparing the relative expression levels of P450 orthologs in several dissected organisms of each species. To compare the sensitivity against major insecticides, lethal doses of major insecticides relevant to both honey bee species were assessed by topical and oral ingestion bioassays. The determined sublethal doses of insecticides were applied to honey bees, and the inducibility of P450s was investigated by comparing the expression patterns of multiple P450s. From these results, this study eventually attempts to compare the toxicological differences between two Apis species with differences in induced cytochrome P450 expression levels.

Canine parvovirus-2 (CPV-2) has been reported worldwide as a major pathogen associated with acute hemorrhagic enteritis. The disease is a major infectious cause of death, particularly in young dogs. The earliest type of CPV-2 was replaced with three main subspecies, CPV-2a, CPV-2b, and CPV-2c, within a few years. Vaccination is carried out regularly, but the emergence of antigenic variants and the influence of maternal antibodies have limited the efficacy of commercial vaccines. New vaccines, such as the subunit vaccine, have been developed for alternative, safe, and effective vaccination. The baculovirus expression vector system (BEVS) is an excellent eukaryotic expression system with a high-level expression of foreign proteins and the ability of post-translational modification. Therefore, it is used widely to produce recombinant protein and subunit vaccines. In this study, the VP2 protein of CPV-2b cloned in the gateway vector system was generated using a baculovirus expression system in Spodoptera frugiperda (SF9) insect cells. Hemagglutination assay (HA) titers (24) were obtained, and the expression was detected in 6-His tagged VP2 and monoclonal antibody (mAb) against CPV-2 by western blotting. The VP2 protein of CPV-2b expressed in this study may provide a basis for a clinical diagnosis and vaccination applications for CPV-2.

Background: Efficient gene editing technology is needed for successful knock-in. Homologous recombination (HR) is a major double-strand break repair pathway that can be utilized for accurately inserting foreign genes into the genome. HR occurs during the S/G2 phase, and the DNA mismatch repair (MMR) pathway is inextricably linked to HR to maintain HR fidelity. This study was conducted to investigate the effect of inhibiting MMR-related genes using CdCl2, an MMR-related gene inhibitor, on HR efficiency in HC11 cells. Methods: The mRNA and protein expression levels of MMR-related genes (Msh2, Msh3, Msh6, Mlh1, Pms2), the HR-related gene Rad51, and the NHEJ-related gene DNA Ligase IV were assessed in HC11 cells treated with 10 μM of CdCl2 for 48 hours. In addition, HC11 cells were transfected with a CRISPR/sgRNA expression vector and a knock-in vector targeting Exon3 of the mouse-beta casein locus, and treated with 10 μM cadmium for 48 hours. The knock-in efficiency was monitored through PCR. Results: The treatment of HC11 cells with a high-dose of CdCl2 decreased the mRNA expression of the HR-related gene Rad51 in HC11 cells. In addition, the inhibition of MMR-related genes through CdCl2 treatment did not lead to an increase in knock-in efficiency. Conclusions: The inhibition of MMR-related gene expression through high-dose CdCl2 treatment reduces the expression of the HR-related gene Rad51, which is active during recombination. Therefore, it was determined that CdCl2 is an inappropriate compound for improving HR efficiency.