PPARα activator가 고지방 사료를 섭취한 운동하지 않은 쥐에 비해 고지방 사료를 섭취한 운 동 쥐에서 백색지방조직의 혈관신생을 보다 효과적으로 억제하는지를 조사하였다. 수컷 쥐는 무작위로 PPARα activator인 fenofibrate와 운동을 모두 처리하지 않은 대조군(Con), fenofibrate 단독처리군(FF), 운동 단독처리군(Ex) 및 fenofibrate와 운동의 조합처리군(Ex+FF)으로 나누어 8주간 고지방 사료를 섭취시 켰다. 백색지방조직의 무게와 백색지방세포의 크기는 Con에 비해 FF, Ex 및 Ex+FF 모두 감소하였으며, Ex+FF는 FF에 비해 더욱 감소하였다. 백색지방조직에서 MMPs와 혈관신생 인자의 유전자 발현은 Con에 비해 FF, Ex 및 Ex+FF 모두 감소하였으며, Ex+FF는 FF에 비해 더욱 감소하였다. 그러나 혈관신생 억제인 자의 유전자 발현은 Con에 비해 FF, Ex 및 Ex+FF 모두 증가하였고, Ex+FF는 FF에 비해 더욱 증가하였 다. 따라서 본 연구는 fenofibrate 단독처리보다는 fenofibrate와 운동의 조합처리가 효과적으로 백색지방조 직의 혈관신생을 억제함으로써 백색지방조직의 증가를 감소시키고 복부비만을 억제한다는 것을 밝혔다.

This study aimed to determine the anti-obesity effect of adding Wolfiporia extensa Ginns (W) to fermented pollack skin products in an obesity-induced animal model. The experimental groups were the normal diet group (C), high-fat diet group (HF), dried pollack skin (H1), fermented pollack skin (H2), and W of 0.1 (F2-WL), 0.3 (F2-WM), and 0.5 (F2), respectively. It was confirmed that adding W to fermented pollack skin reduced blood triglycerides, total cholesterol, and LDL levels, while increasing HDL levels. Wolfiporia extensa Ginns was effective in controlling weight and improving blood lipids in a dose-dependent manner. In histological analysis, findings of fatty liver induced by a high-fat diet were improved by the addition of H2 and W. Size and density of fat globules in the epididymis were decreased. In addition, the concentration of TNF-α was increased in the high-fat diet group, but decreased by the addition of fermented pollack skin and W. In conclusion, adding fermented dried pollack skin and Wolfiporia extensa Ginns was effective for weight control and blood lipid improvement. Thus, the use of by-products in functional foods is expected to have a high value in the future.

본 연구에서는 T2 터보 스핀 에코 지방 포화 기법 중 딥러닝 기반 T2 터보 스핀 에코 Dixon 기법에서 지방분율에 대한 분석을 통해 정확한 지방 포화가 이루어지는지 알아보고자 하였다. 이에 미국 방사선학회 인증 팬텀을 기준 팬텀으로 설정 하고, 액체 지방 팬텀을 이용하여 일반 T2 지방 포화 기법들과 딥러닝 기반 T2 Dixon 기법의 지방분율을 정량적으로 분석 하였다. 연구 방법은 기준 팬텀 3시 방향에 지방 함유율이 0, 10, 20, 30%인 액체 지방 팬텀을 고정하고, 기법별 액체 지방 팬텀 중심부의 신호강도 값을 도출하였다. 그리고 측정된 값을 지방분율 공식을 이용하여 수치화하였다. 연구 결과 각각의 액체 지방 팬텀의 지방분율 측정에서 T2 Dixon 딥러닝 기법과 Dixon 기법이 다른 일반 지방 포화 기법들과 비교해 기준 지방분율에 가장 근접하였다. 그리고 두 기법 간 통계적 차이는 없어 딥러닝 영상 재구성이 지방 포화에 영향을 미치 지 않음을 알 수 있었다. 따라서 딥러닝 기반 T2 터보 스핀 에코 Dixon 기법은 정확한 지방분율로 지방 포화를 할 수 있어 그 유용성이 있다고 생각한다.

This study investigated the anti-obesity effects of Coriandrum sativum L. ethanol extracts in a high fat diet-induced obesity model (DIO). We confirmed the anti-obesity effects by analysing the expression of the related proteins, weight gain, dietary intake, dietary efficiency, blood biochemistry, histological analysis and western blot analysis. After oral administration of Coriandrum sativum L. ethanol extracts at concentrations of 250 and 500 mg/kg, a significant improvement in dietary efficiency, reduction in weight gain, triglycerides, total cholesterol and LDL-cholesterol in blood lipid was observed for 8 weeks. In addition, improvement in blood glucose and metabolism confirmed through glucose tolerance test was observed. Further, the concentration of alanine transaminase (ALT) in blood was significantly decreased, which improved the fatty liver caused by high-fat diet intake as confirmed by liver tissue analysis. This phenomenon was confirmed to decrease the expression of fat accumulation-related PPARγ and FAS protein in the liver tissue. Especially, it is believed that FAS, a liposynthetic enzyme, has a stronger inhibitory effect than PPARγ. Therefore, Coriandrum sativum L. ethanol extract is thought to improve obesity by reducing blood lipids levels, improving glucose metabolism and inhibiting synthesis of the fat that accumulates in the liver in high-fat diet-induced obesity animal models.

Physicochemical properties and storage stability of plant-based alternative meat prepared with low-fat soybean powder (LPAM) treated by supercritical-CO2 and those of full-fat soybean powder (FPAM) were compared. Ash and crude protein contents were higher in LPAM than in FRAM. Water absorption capacity and oil absorption capacity were significantly higher in LPAM than in FPAM. Water binding capacity was higher in LPAM than in FPAM during a 20 days storage period at 5℃ and pH was significantly lower in LPAM than in FPAM after a 5~10 days storage period. Hardness, gumminess and chewiness significantly increased with the increase in the storage period, and the three were significantly higher in LPAM than in FPAM after 10 days and 20 days of storage. The acid value showed no remarkable difference according to the storage period in LPAM; however, it was significantly higher in FPAM than in LPAM after 20 days of storage. The peroxide value and TBA value were significantly increased according to the storage period, and were significantly lower iin LPAM than in FPAM during all the storage periods. Therefore, the use of low-fat soybean powder may be effective in improving oxidative stability during storage in the production of plant-based alternative meat.

Toll-like receptor 4 (TLR4) is known to contribute to the modulation of insulin resistance and systemic inflammation seen in obesity and the metabolic syndrome. The present study was performed to investigate the fertility competence of TLR4 knock out male mice (TLR4 mice) on a high-fat diet (HFD), compared to a normal-chow diet (NCD). The controls included wildtype (WT) mice fed on a HFD or NCD. Six-week-old male mice were fed with either a NCD or HFD for 20 weeks. Body and organ weights, serum levels of glucose, triglycerides and hepatoxicity, sperm quality and spermatogenesis were observed after the sacrifice. Also, randomly selected male mice were mated with virgin female mice after feeding of 19 weeks. The weight of the body and organs increased in WT and TLR4 mice on a HFD compared to those of mice on a NCD. The weights of the reproductive organs did not vary among the treatment groups. The motility and concentration of the epididymal spermatozoa decreased in both WT and TLR4 mice fed a HFD. The pregnancy rate and litter size declined in the HFD-fed WT mice compared to the HFD-fed TLR4 mice. In conclusion, the HFD alters energy and steroid metabolism in mice, which may lead to male reproductive disorders. However, fertility competence was somewhat restored in HFD-fed TLR4 male mice, suggesting that the TLR4 is involved in testis dysfunction due to metabolic imbalance.

본 연구는 발가락 지방 소거 검사 시에 종종 저품질의 영상이 획득되는 경우에 주목하여 다양한 조절인자들을 조절하지 않아도 손쉽게 영상 품질을 향상하는 방법을 고안해보았다. 팬텀과 지원자를 대상으로 발 전용 코일만 사용한 경우와 추가 코일을 결합 사용한 경우의 영상을 획득 후 신호대 잡음비(SNR)를 측정하여 비교하였다. 시상면, 관상면, 축상면 모두 T2 Dixon을 시행하였고, 시상면의 경우 T2 지방 소거(FS)와 T1 지방 소거를 추가 시행하였다. 팬텀 검사에서 추가 코일을 결합 사용 시 SNR은 시상면의 경우 T2 Dixon water는 1.21배, T2 FS은 1.22배, T1 FS은 1.17배 향상되었다. 관상면 T2 Dixon water는 1.15배, 축상면 T2 Dixon water는 1.47배 향상되었다. 지원자 검사에서 추가 코일을 결합 사용 시 SNR은 시상면의 경우 T2 Dixon water는 2.07배, T2 FS은 5.17배, T1 FS은 3.20배 향상되었다. 관상면 T2 Dixon water는 1.20배, 축상면 T2 Dixon water는 1.37배 향상되었다. 추가 코일을 결합 사용하였을 때 모든 지방 소거 검사 및 모든 방향에서 SNR 향상의 결과를 얻을 수 있었다. 결론적으로 사용자의 고도 기술 및 추가 검사 시간이 필요하지 않고도 SNR을 향상할 수 있음을 의미한다.

본 논문의 목적은 짜라까 상히따에서 근육조직과 지방조직의 손상에 의한 질병 치료법 연구이다. 아유르베다에서 가르치는 모든 치료법의 목표는 세 도샤의 균형 상태를 유지하 는 데에 있다. 건강은 규정된 질병이 없는 상태를 의미하는 것이 아니라 도샤, 조직 그리고 노폐물이 적절하게 기능적으로 균형을 이룰 때라고 정의한다. 그렇지만 질병의 근원은 이 들이 불균형상태를 말하며 그것의 근본원인은 독소이다. 근육조직은 근육물질을 제공하여 뼈를 지탱하고 활동 기능과 역할이 있다면 지방조직은 지방물질을 제공하며 윤활작용을 하 는 기능과 세포막을 구성하는 역할을 한다. 이러한 근육조직과 지장조직의 손상원인은 지 나치게 음식물을 많이 섭취하거나 또는 적게 섭취하는 경우이다. 그들의 손상에 따른 질병 을 살펴보면 근육조직은 육아종과 근종 등이 있고 지방조직은 당뇨병과 소변질환 등이 있 다. 근육조직과 지방조직의 손상에 의한 질병 치료법으로는 각각 찜질요법과 8가지 바람직 하지 않은 사람에서 찾는다. 먼저 찜질요법의 상당부분은 지난 번 연구에서 다루어졌기 때 문에 그것을 제외한 소개가 안 된 내용만 소개하였다. 지방조직은 비만인 경우와 저체중인 경우로 나누어 단식요법과 영양요법으로 관리를 하면 되지만 이들 요법은 지난번에 연구논 문에서 언급되었기 때문에 자세한 내용을 지난번 연구에 미루고 생략하였다.

Sirtuin 1 (SIRT1), the most conserved nicotinamide adenine dinucleotide-dependent protein deacetylase, is involved in the regulation of energy metabolism, genomic stability, and development. SIRT1 knockout (SIRT1) mice exhibit decreased energy expenditure and hypersensitivity to a high-fat diet (HFD). SIRT1 deficiency in the testis has also been shown to cause male infertility in animal models. Therefore, the present study was conducted to examine the alteration of the testicular function of SIRT1 mice on HFD. Six-week-old mice were fed ad libitum as wild type (WT) and SIRT1 male mice with either a control diet or with HFD for 32 weeks and then were sacrificed. The levels of biomarkers for hepatotoxicity, sex hormones, and cytokines were analyzed in the serum and blood-testis barrier, and the sperm morphology was examined in the testis and epididymal spermatozoa. Interestingly, an enlargement of seminal vesicles was observed in the SIRT1 mice fed with HFD. A significantly higher level of hepatotoxicity was also seen in these mice. The concentration of serum testosterone increased in HFD-fed SIRT1 mice compared to the controls. The levels of interleukin-1β and TNF-α increased in both HFD-fed WT and SIRT1 mice. In RT-PCR, the m RNA expression of tight junction protein 2 and claudin 3 significantly decreased in HFD-fed SIRT1 compared to those of the controls. Degenerative spermatocytes and spermatids were detected in the HFD-fed SIRT1 mice testicular section. Sperm motility decreased in WT and SIRT1 with HFD feeding, and sperm concentration decreased significantly in WT-HFD and SIRT1 mice with or without HFD feeding. Taken together, HFD can alter energy and steroid metabolism in SIRT1-deficient mice, which can lead to imbalances in motility and production of sperm and testosterone that can result in male reproductive disorders.

The physicochemical properties of the emulsions prepared with four different proteins (pea protein isolate, whey protein isolate, soy protein concentrate, and soy protein isolate) were characterized in terms of particle size, rheological property, and freeze-thaw stability, while the feasibility of the protein-stabilized emulsions as a fat replacer was evaluated. Confocal laser scanning microscopic analysis showed that O/W emulsions were successfully prepared with all the proteins; however, the smallest particle size and excellent syneresis were observed in the WPI-stabilized emulsions. The use of WPI-stabilized emulsions as an alternative for shortening up to 50% level did not lead to differences in the muffin batters’ specific gravity and viscoelastic properties. After baking, the substitution of shortening with the WPI emulsions at up to 50% by weight did not significantly affect the muffins’ specific volume and texture characteristics.