본 연구는 기능성 곡물로 이용되고 있는 아마란스(Amaranthus ssp.)를 어린잎채소 용도로 개발하기 위하여 국내에 보유되어 있는 유전자원에 대한 어린잎 생육특성, 엽 적색도 발현 정도 및 기능성 물질의 함량 변이를 평가함으로써 어린잎채소로서 이용 가능한 유망 아마란스 자원 선발을 위하여 수행되었다. 국내에 수집․보존되어 있는 아마란스 유전자원 262 종에 대하여 색도색차계로 측정한 엽 적색도 발현 정도를 기준으로 전체 자원의 상위 15%에 해당하는 39종을 1차 선발하였다. 1차 선발된 유전자원에 대하여 고온기 및 저온기의 재배를 통하여 어린잎 생육과 유전자원 별 적색소 발현을 검증 평가하여 안정적인 적색 강도를 보이는 고색도 자원을 최종 선발하였다. 아마란스의 엽 적색도는 모든 1차 선발 계통들에 있어서 고온기에 비해 저온기 재배 시 적색도 발현이 우수하였으며 2회의 재배를 통한 평균 적색도 발현 정도를 기준으로 IT199970 등 11종의 자원을 선발하였다. 이들 선발된 유전자원들에 대한 어린잎 생체중 및 총페놀 함량, 총플라보노이드 함량 정보를 제시하였다. 이들 선발 종들은 5개의 아마란스 종에 속하였으며 엽 적색도 발현이 우수하여, 향후 적색 풍미를 더하는 어린잎 샐러드 채소의 소재로서 이용 가능할 것으로 판단된다.

Meedon rice varieties are important for local adaptability, grain quality and market availability, and have been grown in Myanmar for centuries. Because of temporal variability and spatial heterogeneity, Meedon rice varieties in rainfed lowland areas may be diverse. However, information on diversity of Meedon rice germplasm is limited. This study was carried out to assess genetic diversity and to analyze population structure of Meedon rice germplasm conserved in Myanmar Seed Bank using SSR markers. For assessing genetic diversity, 154 accessions of Meedon rice germplasm were analyzed with nine SSR markers. A total of 86 alleles were detected with an average of 9.6 alleles per locus. All the loci were found to be polymorphic, and there were considerable genetic variation among accessions with mean values of expected heterozygosity (HE) = 0.5774 and polymorphic information content (PIC) = 0.5496. High frequency of rare alleles was identified, among which 35 unique (accession-specific) alleles were observed. Based on cluster analysis, rice accessions were mainly clustered into two groups, and as a result of model-based analysis, two distinct genetic populations and an admixture were classified. This result indicated that SSR markers have proved to be useful markers for detecting genetic diversity in Meedon rice, and the occurrence of a considerably high number of rare and unique alleles in the germplasm indicates their potentiality as a reservoir of rare genotypes for use. Unique alleles are also important because they may be diagnostic of a particular type of genotype for identification.

Genomic DNA prepared from fruitbdies of 25 Grifola frondosa strains were amplified with the ITS primers and the PCR reaction products were enzymed. The ITS bands have same electrophoresis patterns but part of them have different restriction enzyme cutting site. These strains were divided into three broad categories. SRAP amplification employing 47 SRAP primer pairs were carried on and 138 polymorphic bands were detected. The phylogram tree showed that: ten strains were differentiated from the others effectively and fifteen strains have no obvious differences between each other. The results implied that the ITS-RFLP and SRAP markers were effective methods for strains identifica tion and germplasm evaluation but other markers were also needed to be used in conjunction.

Sorghum-Sudan grass which is an annual forage grass in Gramineae have been bred for biomass promotion. The recombinant inbred line (RIL) populations were derived from the F2:3 genetic mapping population of Sorghum 314A×zongke Sudan grass. The breeding selection based on agronomic characters and DNA molecular marker statistical analysis was carried among the progenies of the RIL populations, and 9 recombinant breeding progenies were determined as heterobeltiosis with high yield. According to DNA diversity analysis of simple sequence of repeats(SSR) marker, the genetic varieties were abundant among the families, and the recombinant breeds of high yield were distributed among the different RIL populations.2 family lines, which have similar agronomic characters and yield, and whose genetic distance nearly equal to zero, showed difference on arid tolerance. 4 breeding lines were selected on agronomic characters and resistance in the field trials, and 2 breed lines were qualified for the regional testing.

39 accessions of sickle alfalfa (Medicago falcata L.) collected from different regions of China were evaluated for their tolerance to drought. Differences were observed among accessions in their reactions to drought and 8 good tolerant accessions at populations level and 18 excellent tolerant accessions at individual level were identified based on drought response index (DRI).

국내외로부터 수집한 고추 유전자원을 포장에 재배하여 종자를 증식하는 동시에 주요 특성을 조사하였다. 헝가리에서 도입하여 1992년도에 평가한 계통들은 Hungarian Wax 혹은 Bell형으로서 바이러스에 심하게 걸려 우리 포장환경에 잘 적응하지 못하였다. 1994년도에 평가한 계통들 중 브라질에서 풋마름병 저항성으로 도입한 KC350과 KC351은 직립성이며 바이러스에 강한 편이어서 매우 유망한 유전자원으로 사료되었다. 중국에서 도입한 KC376는 바이러스에 강한 편이며 대과를 착생하여 유망하게 보였다. 태국에서 도입된 계통들은 안동대에서 특성을 평가기록하였다.

우리나라에서 Korean lespedeza의 자생지에 대한 작물의 생육상태, pH, 무기 및 유기물 함량, 토양입자, 토성 등과 토양의 물리-화학성에 관한 기초자료를 얻고져 총 62개 지역을 조사하였으며 얻은 자료를 분석한 결과는 다음과 같다. 1. Korean lespedeza의 초장 및 건물량은 조사시기 및 지역에 따라서 큰 차이를 보였으며, 8월 하순 및 9월 상순에 조사한 수치가 높았다 2. 식물체내의 영양분 함량에 있어서 지상부는 지하부보다 N

한국재래종과 도입종을 포함한 62계통의 고추를 경북대학교 부속농장에 재배하여 종자를 증식하는 동시에 개화소요일수를 포함한 16가지 형질의 특성을 조사 기록하였다. 개화소요일수에 있어서는 KC85(칠곡 동명)와 KC116(PI308791), KC154(Cherry Sweet) 및 KC185(조선고추)가 83일로 가장 조생이었고 KC139(Serrano Chili)와 KC103(PI297488)이 126일로 가장 만생이었다. 과실을 따는데 걸리는 힘에 있어서는 KC85(칠곡 동명)가 0.45kg으로 가장 쉽게 따지고 KC162(PI224444)가 3.34kg으로 가장 많은 힘이 걸렸다. 흰가루병에는 KC10(Pimiento), KC23(PI224423), KC51(PI257044), KC56(PI257053), KC126(PI358812), KC119(PI322719), KC143(Emerald Giant), KC148(VR-2), KC163(PI224445), KC166(Papari Mild)이 발병이 적었다. 탄저병 발생은 KC39(Serrano Chili)에서 가장 적었다. 고추 품종의 수집 및 도입활동을 계속하여 한국재래종 14점과 도입종 25점을 추가 확보하였다.

High-quality and high-phytonutrient watermelon fruits have strong market opportunities besides their health related benefits. Hence, investigating quality and nutritional related traits of watermelon genetic resources could provide important baseline data in breeding for increased lycopene content thereby increasing the marketability of watermelon. To this end, we have examined some fruit morphological traits and lycopene content of 105 genetic resources. Seeds, originally obtained from 22+ countries, were obtained from the National Agrobiodiversity Center, Jeonju, South Korea, grown in an experimental field and harvested at a fully mature stage. The size of pistil scar (SPS), the width of stripes (WS), weight of fruit (WF), length of fruit (LF), width of fruit (WIF), the thickness of pericarp (TP), soluble solids content (SSC), fruit shape in longitudinal section, ground color of skin, the intensity of the green color of skin, fruit shape at the apical part, grooving distribution, conspicuousness of stripes, and main color of the flesh were recorded on the field and inside laboratory and the lycopene was measured using spectrophotometric and HPLC methods. Watermelon fruits have shown a diverse morphological characters. Red and pink fleshed fruits dominated in the entire collections. Fruits with higher thickness of rind were found to exhibit less soluble solid content (SSC). Korean origin fruits were characterized by intermediate SSC while the United States of America (USA), Russia (RUS), Tajikistan (TJK), Turkmenistan (TKM), Taiwan (TWN), and Uruguay (URY) originated fruits had the highest SSC. The lycopene content varied between 41.37 and 182.82 ㎍/g, 2.81 and 163.72 ㎍/g, and 3.54 and 255.47 ㎍/g using HPLC, UV-Vis spectrophotometer, and microplate reader spectrophotometer, respectively. Red- and pink-fleshed fruits had the highest levels of lycopene content compared to the yellow- and orange-fleshed. Lycopene content had a significant positive correlation with SSC, however, no correlations were detected between lycopene and other quantitative fruit morphological characters. Our study demonstrated high diversity exists in fruit morphological traits and lycopene content of the germplasm collections which provide beneficial baseline data for a future breeding program and utilization of watermelon germplasm collections in gene banks for the maintenance and improvement of the current levels of production, marketability, and health-related benefit of watermelon fruits.

Sclerotinia rot, caused by Sclerotinia sclerotiorum, is a devastating disease that poses a serious threat to perilla production in Korea. Identifying effective sources of resistance offers long term prospects for improving management of this disease. Screening disease resistant genetic resources is important for development of disease-resistant, new cultivars and conduct related research. In the present study, perilla germplasm were screened in vitro against S. sclerotiorum using detached leaf method. Among 544 perilla accessions, two were highly resistant (IT226504, IT226533), five were resistant (IT226561, IT226532, IT226526, IT226441, and IT226589), five were moderately resistant (IT226525, IT226640, IT226568, IT220624, and IT178655), 16 were moderately susceptible, 31 were susceptible, and 485 were highly susceptible. The resistant accessions in this study could serve as resistance donor in the breeding of Sclerotinia rot resistance or subjected to selection procedure of varietal development for direct use by breeders, farmers, researchers, and end consumers.

본 연구는 이란 등 5개국에서 수집한 홍화자원을 대상으로 하여 천연항산화제로써 홍화종자의 항산화 활성을 평가하고, 세로토닌 유도체인N-(p-Coumaroyl) serotonin과N-feruloylserotonin의 함량을 측정하여 항산화 활성과의 상관관계를 조사하였다. 총폴리페놀함량은 28.25 ~ 90.53 ㎍ GAE/㎎ DE의 범위를 나타내었고, 색소화합물인 DPPH 라디칼과ABTS+을 이용한 유리라디칼소거능을 측정하는 DPPH assay와 ABTS assay 의 값은 18.76 ~ 93.98 및 48.91 ~ 163.73 ㎍ ASCE/㎎ DE의 범위를 나타내었다. 시료의 환원능을 측정하는 방법인 FRAP assay 과 RP assay는 3.80 ~ 132.29 및 26.32 ~ 80.08 ㎍ ASCE/㎎ DE의 범위로 나타내었다. UHPLC를 이용하여 CS와 FS를 분석한 결과, CS 함량은 2.56 ~ 64.99 ㎎/g DE이고, FS 함량값은 1.92 ~ 65.36 ㎎/g DE이었다. 5개국 중 이란 원산종은 다른 나라에 비해 세로토닌 유도체 함량과 항산화 활성의 평균값이 높게 측정되었다. 항산화 활성과 세로토닌 유도체 함량 간의 상관관계 분석 결과, CS는 TPC와 ABTS, DPPH와 높은 상관성을 보였고(r = 0.673, 0.727, 0.820), FS는 DPPH와 높은 상관성을 보였다(r = 0.740). RACI를 통하여 선발된 IT321214과 IT321215는 항산화 활성이 높은 자원으로 천연항 산화제 소재 탐색 및 개발에 기초자료로 활용할 수 있을 것으로 생각된다.