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        검색결과 17

        1.
        2024.04 구독 인증기관·개인회원 무료
        Climate change has made outbreaks of insect-transmitted plant viruses increasingly unpredictable. Understanding spatio-temporal dynamics of insect vector migration can help forecast virus outbreaks, but the relationship is often poorly characterized. The incidence of Beet curly top virus (BCTV) was examined in 2,196 tomato fields in California from 2013-2022. In addition, we experimentally showed dispersal of the beet leafhopper, the only known vector of BCTV is negatively correlated with plant greenness, and we estimated spring migration timing using a vegetation greenness-based model. Potential environmental factors and spring migration time of beet leafhoppers were associated with BCTV incidence. We found BCTV incidence is strongly associated with spring migration timing rather than environmental factors themselves. In addition, the vegetation greenness-based model was able to accurately predict the severe BCTV outbreaks in 2013 and 2021 in California. The predictive model for spring migration time was implemented into a web-based mapping system, serving as a decision support tool for management purposes.
        2.
        2017.10 구독 인증기관·개인회원 무료
        Vector-borne plant virus transmission is a complex mechanism. Plant viruses modify development and behavior of vectorinsects in a positive, negative, or neutral manner. Bemisia tabaci transmits Tomato yellow leaf curl virus (TYLCV) whichis a virus that seriously damaged tomato cultivars all around the world. We compared several behavioral and physiologicalcharacteristics between non-viruliferous (NV) and TYLCV-viruliferous (V) of whiteflies. When B. tabaci acquired TYLCV,total life span was shorter and fecundity was lower than NV ones. V whiteflies were more susceptible to thermal stressby increasing hsp mRNA levels but higher in mortality by either heat or cold shocks. Further, V whiteflies increasedthe rates of plant sap probing and light attraction behaviors than NV ones. Our results provide insights to understandvector’s role in relation to the acquisition and transmission of plant viruses.
        3.
        2017.10 구독 인증기관·개인회원 무료
        The relationship between virus, vector insect and its host plant has been studied in epidemic of virus disease, fitnessof vector insect, symbiosis and phylogenesis of the relationship, etc. The biological association, symbiosis, concept hasbeen suggested on the virus-vector relationship since 2000. We have conducted such a typical study with the Rice stripevirus and the small brown planthopper, Laodelphax striatellus. In addition, it is able to extend our view to ecosystemservice of the relationship. We reviewed the trophic efficiency included with virus factor. Nutrient loss of the host plantby virus is negative effect but terrestrial decomposition by the virus may be positive feedback in ecosystem. Thus, thevirus-vector relationship is able to be understand not only as competition for the host plant’s nutrition but also as indirectmutualism for the efficient utilization of the host plant or fitness to environmental conditions, especially climate change.
        4.
        2017.10 구독 인증기관·개인회원 무료
        Different types of insect-borne plant viruses can modify their hosts and vectors in distinct manners. Therefore, interactionsbetween two types of viruses co-existing in a field are known to be complex to predict. Obtaining empirical data byconducting field experiments, however, requires numerous biotic and abiotic factors to be controlled, and is therefore hardto execute. Thus, we designed an individual based model to simulate the transmission pattern of two viruses, using potatoes(Solanum tuberosum) for host plant, aphids (Myzus persicae) for virus vector, potato leafroll virus and potato virus Yfor different types of plant viruses. More specifically, we aimed to investigate the effect of the following on the spreadof the plant viruses: dispersal by winged-form aphids, initial number of virus-infected seed potatoes, and indirect interactionsbetween two viruses by affecting life traits of the vectors.
        5.
        2017.04 구독 인증기관·개인회원 무료
        The interactions of vector-virus-plant have important ecological and evolutionary implications. In this presentation, I will use the case studies on the whitefly vectors, begomoviruses, and plants to illustrate the complexity, consequences and mechanisms of this type of tripartite interactions. The interactions between begomoviruses and their whitefly vectors via their shared host plants can be mutualistic, neutral or negative depending on the species/strains of each type of the organisms involved. With regard to the mechanisms of plant-mediated positive effects of the viruses on whiteflies, three case studies indicate that suppression of jasmonic acid/salicylic acid related plant defence plays an important role. Our recent studies show that the order of arrival of the interacting vector insects and viruses on the plants may also alter the physiological feature and consequences of the interactions. Future efforts in this area should try to expand the number and diversity of case studies in order to reveal the patterns of interactions, to unravel the molecular and biochemical mechanisms of the interactions using a multidisciplinary approach, and to examine the virus-plant-vector interactions in the field and in natural plant communities.
        6.
        2013.10 구독 인증기관·개인회원 무료
        Acquisition of plant viruses has various effects on physiological mechanisms in vector insects. Bemisia tabaci is the only known vector of Tomato yellow leaf curl virus (TYLCV), which is a serious virus affecting tomato cultivars. In this study, the lifespan of Q1 biotype was compared between non-viruliferous (NV) and TYLCV-viruliferous (V) whiteflies. Total lifespan from egg to adult death of NV whiteflies was 62.54 days but 10.64 days shorter in V whiteflies. We investigated the temperature susceptibility of B. tabaciby comparing mortalities as well as heat shock protein (hsp) mRNA levels between NV and V whiteflies. For this, NV and V whiteflies were exposed for either 1 or 3 h at 4, 25, and 35 °C. The mortality of V whiteflies was higher than NV ones following exposure at either 4 or 35 °C, but there was no significant difference at 25 °C. Analysis of the expression level of heat shock protein (hsp) genes using quantitative real-time PCR showed that both cold and heat shock treatments stimulated higher expression of hsps (hsp40, hsp70, and hsp90) at various rates in V whiteflies than NV ones, but there was no difference at 25 °C. All together, our results show that TYLCV acquisition accelerated the developmental rate and increased susceptibility to thermal stress in B. tabaci. Therefore, this modification may result in reduced vector longevity due to increased metabolic energy utilization. Our results provide insights into the complex interaction between vector fitness and thermal stress in relation to the acquisition and transmission of plant viruses.
        7.
        2010.10 구독 인증기관·개인회원 무료
        Recently, several significant plant virus diseases have devastated various crops in agricultural fields of Korea, These virus disease are mostly transmitted and spread by vector insects, such as rice stripe virus (RSV) transmitted by planthopper Laodelphax striatellus, tomato spotted wilted virus (TSWV) by thrips Frankliniella occidentalis and tomato yellow leaf curl virus (TYLCV) by whitefly Bemisia tabaci. Insects as vectors are significantly important for the control of plant disease as well as insect themselves. Here, on the view of insects, interactive mechanisms of vector insect and plant pathogens were discussed on the system of TYLCV and B. tabaci. When insects acquisited virus they would be beneficial, neutral or detrimental. Generally, plant virus are ingested into the mouse and move from the gut to hemolymph and back to salivary gland. During this movement there would be numerous molecular interactions at a vector-virus specific mode. In addition, virus acquisition change vector's physiological and developmental states including longevity, fecundity and dispersal behavior. However, these interactive mechanisms of vector insects with plant virus are largely under investigated. B. tabaci is genetically diverse and transmit many begomoviruses including TYLCV. I demonstrated effects of TYLCV acquisition on fecundity, thermal response, endosymbiont profile and gene expression of B. tabaci.
        8.
        2008.10 구독 인증기관·개인회원 무료
        The sweet potato whitefly Bemisia tabasi is one of the most important pests of various horticultural crops. In addition, B. tabaci is a vector of many plant-pathogenic viruses and cause a serious secondary damage to crop plants. Association of plant-pathogenic virus with vector insects is known to be effective on the transmission capacity, fecundity, longevity of vectors including whiteflies. However, the interactive mechanisms between virus and vector insects are still poorly understood. Recently, a serious damage caused by virus disease together with B. tabasi emergence was identified at tomato glasshouse in Tongyoung. We detected the signals of Tomato Yellow Leaf Curl Virus (TYLCV) in tomato leaves and vector whiteflies using PCR amplification and confirmed its presence by those sequence comparison. To determine the effects of TYLCV acquisition on physiological status of vector whiteflies, transcript levels of genes that associated with metamorphosis, metabolism, stress and immune processes were compared between TYLCVinfected whiteflies and non-infected ones. Generally, the transcript levels of virus-infected whiteflies were lower than those of non-infected ones. In addition, the associations of endosymbiont levels within whiteflies were discussed in aspect of the acquisition and transmission of TYLCV.
        9.
        2008.10 구독 인증기관·개인회원 무료
        For the physiological study on environmental impact of genetically modified (GM) pepper plant on non-target but three-trophically related insect species, we investigated behavioral responses of Aphis gossypii and Aphidius colemani in Y-tube olfactometer to the cucumber mosaic virus (CMV)-resistant transgenic pepper plant (H15 GM line) expressing coat protein gene of CMV and its wild type pepper plant (untransformed, susceptible to CMV pathotype II, P2377 inbred line) in relation to CMV infection. CMV-infected plants were prepared with the 30 min of inoculation by the winged A. gossypii viruliferous or mechanical inoculation using CMV-Fny, and with molecular diagnosis using reverse transcriptase-polymerase chain reaction (RT-PCR) over 2 weeks after inoculation. In this study, time for attraction responses (attraction time) of A. gossypii were not significantly different in the pepper strain, and the virus infection of plant. However, the attraction time of A. colemani was significantly different between the GM plant and the non-GM plant. In addition, the attraction time of A. colemani to the GM plant was significantly decreased according to the CMV infection. For further study, the volatile organic compounds (VOCs) emitted by these plants were collected with an entrainment kit and analyzed by Gas Chromatography (GC) on HP-1 column. The specific VOCs related to CMV infection were detected in the GM plant over 4 weeks after inoculation of CMV in this study. Thus, it is suggested that VOCs of the GM plant in this study may be produced more as a signal attracting A. colemani in relation to CMV infection.
        11.
        1975.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        우리나라 잎담배에 발생하고 있는 바이러스의 종류와 감염상을 조사하기 위하여 전주지방에서 Burley종 (Burley-21) 20개체, 부천연초시험장 포장에시 황색종(Hicks) 20개체를 병징별로 채집하여 혈청학적인 방법에 의해 실시했다. 검정대상 바이러스는 Tobacco mosaic virus(TMV), Cucumber mosaic virus(CMV), Alfalfa mosaic virus (AMV), Potato virus X(PVX) 및 Potato virus Y(PVY)등 5종으로 TMV, AMV, CMV는 한천내확산법으로 PVX, PVY는 미량침강법으로 실시하여 다음과 같은 결과를 얻었다. 1. 공시이병개체중에서 TMV, CMV, AMV, P X 및 PVY 등 5종의 바이러스가 검출되었다. 2. 조사된 40개체중 각 바이러스의 감염율은 AMV , CMV , TMVPVY, PVX의 순이었다. 3. Burley종에서는 TMV감염율이 로 황색종의 보다 현저히 낮았다. 4. 조사된 각 개체는 단독감염으로부터 2-4종의 바이러스로 다양하게 혼합감염을 나타냈으며 이 중 단독감염이 , 혼합감염이 이었다.
        4,000원
        12.
        2013.04 KCI 등재 서비스 종료(열람 제한)
        본 연구는 바이러스 무병묘의 대량 증식을 위한 기초 자료를 얻고자 포도 '자랑' 품종의 신초와 뿌리의 형성 및 생장에 미치는 배지조성물질과 생장조절제의 영향을 구명하고자 수행되었다. 배지의 무기염 농도가 낮을수록 신초의 형성은 많아지는 경향으로, 특히 1/2MS배지에서 신초의 형성 및 생장이 가장 양호하였다. 부정근의 형성은 무기염의 농도가 높아질수록 양호하여 2MS배지에서 부정근의 수가 2.1개로 가장 많았다. Sucrose의 경우, 신초의 형성은 1%, 부정근의 형성은 3%, 생장은 1% 농도구에서 가장 좋았다. 활성탄의 0.05% 첨가에 의해 신초의 생장은 양호했으며, 특히, 부정근의 형성 및 생장에 효과적이었다. '자랑'의 대량 증식을 위한 배지의 pH는 6.8이 가장 적합하여 신초의 마디수와 길이가 각각 3.9개와 1.3 cm로 가장 좋았다. 기내 대량증식에 미치는 생장조절제의 영향으로 BA 1.0 mg/L에서 신초의 생장이 가장 왕성하였으며, NAA 1.0 mg/L에서 절편체당 16.9개의 신초가 형성되어 양호한 결과를 얻었다.
        13.
        2012.09 KCI 등재 서비스 종료(열람 제한)
        고구마의 연작장해를 경감시키기 위하여, 연작지 토양과 심토반전 토양에서 농가묘와 바이러스 무병묘를 70×25cm 간격으로 재배하였다. 연작지 토양의 시비량은 N-P2O5-K2O = 55-63-156kgha1과 우분퇴비 10tonha1으로 표준시비를 하였고, 심토반전 토양은 질소비료와 퇴비만을 50% 증시하였다. 삽식 30, 120일째의 생육과 수량 및 품질특성을 조사한 결과는 다음과 같다. 1. 삽식 30일경부터 농가묘에서는 바이러스 병징이 뚜렷하였으나, 무병묘에서는 나타나지 않았다. 2. 무병묘의 수량성은 농가묘보다 심토반전 토양 15.0%, 연작지 10.5%의 증가를 보였다. 3. 심토반전 토양에서의 수량성은 연작지보다 농가묘 8.8%, 무병묘 3.2%의 증가를 보였으며, 심토반전 토양에서 바이러스 무병묘 재배는 상저비율이 농가묘(60.1%) 대비 80%로 높아져 경제성이 있었다. 4. 무병묘에서 수확한 고구마의 품질은 농가묘보다 피색이 선명하고, 고구마 모양이 좋아져 외관 품질향상에도 유리하였다. 5. 심토반전 토양 및 무병묘에서 수량증가는 30일째 엽수와 120일째 분지수와 정의 상관관계(p=0.05)가 인정되었으며, 이는 고구마 괴근형성에 초기생육이 중요하다는 것을 보여주었다.
        14.
        2011.12 KCI 등재 서비스 종료(열람 제한)
        Pepper mild mosaic virus (PMMoV) and cucumber mosaic virus (CMV) are important pathogens in various vegetable crops worldwide. We have found that methanol extracts of Quercus dentate (Daimyo Oak) gallnut strongly inhibit PMMoV and CMV infection. Based on this result, the inhibitor named as "KN0912" formulated from the extract of Q. dentate gallnut was tested for its inhibitory effects on PMMoV or CMV infection to each local lesion host plant (Nicotiana glutinosa; PMMoV, Chenopodium amaranticolor; CMV). Pre-treatment effect of KN0912 against infections of each virus to local host plant was measured to be 75. 1:±0. 5-97. 5±1. 5% to PMMoV and 70.6±2.2-99.0±1.0% to CMV in 1-10mg/ml conc. and the absorption effect of the antiviral composition of KN0912 to the inside of tobacco leaves tissue, was inhibited by 55.7% to PMMoV and 63.8% to CMV. The persistence of KN0912 treatment was maintained until after the 3 days high inhibitory effect by 98% to PMMoV and by 95.1% to CMV. Inhibitory effects on systemic host plants of KN0912 were measured to be 80-90% to PMMoV and 60-75% to CMV. From the change of morphological characteristics of PMMoV particles under EM, we are tentatively suggested that one mode of action of KN0912 is inactivation due to the destruction of virus particles.
        15.
        2004.10 KCI 등재 서비스 종료(열람 제한)
        본 연구에서는 카네이션과 고구마의 무병 종묘를 생산하고자 정단분열조직배양을 실시하였으며, 생산된 배양종묘의 바이러스 감염여부를 진단하기 위해 ELISA방법을 이용하여 주요 바이러스를 검정하였다. 카네이션의 정단분열조직 배양에 공시 한 'Roland' 등 4품종 중 투명화 현상이 없이 가장 정상적인 잎이 발생하고 절간신장이 이루어지며 뿌리의 발생도 양호한 생장반응을 보인 품종은 석죽계통인 'Giant Gipsy' 이었으며 MS기본배지에 NAA 0.05 ㎎/L와 BA 0.1㎎/L 혹은 kinetin 1.0㎎/L와 조합한 구에서 shoot의 재생이 양호하였다. 배양7주일 후에는 explant 1개체에서 평균 5-6개, 많은 것은 10개체 이상의 multiple shoot가 형성되었고 기내 마디삽목법에 의하여 대량증식할 수 있었다. 투명화현상이 심한 품종은 'Casha'와 'Desio'로 나타났는데 투명화 현상은 kinetin 첨가보다는 BA첨가구에서 현저하게 증가하였다. 카네이션 기내 배양종묘의 바이러스 감염여부는 ELISA방법을 이용하여 CarMV와 CarRSV를 신속하게 다량의 시료를 진단할 수 있었다. 그 결과 정단분열조직의 배양을 통해 65.2%에서 무병 주가 생산되었음을 확인할 수 있었다.
        16.
        1997.04 KCI 등재 서비스 종료(열람 제한)
        팥 바이러스병에 대한 기초자료를 얻고자 병징의 유형과 생육 단계별 감량정도 및 수량에 미치는 영향을 조사하고 병징 유형별 바이러스를 분리 동정한 결과는 다음과 같다. 1. 팥에 발생되는 바이러스병의 병징은 크게 mosaic, yellow mosaic 및 severe mosaic의 3가지 군으로 분류되었으며 병징별 분포는 mosaic>severe mosaic>yellow mosaic 순이었다. 2. 성숙기까지 매개충 차단 재배시의 이병률은 1.5%(방임구 20.7%)로서 생육 후기에 감량될수록 이병률이 낮았으며 10a당 수량도 171kg으로서 방임구에 비하여 45% 증수되었다. 3. 지표식물 검정 결과 mosaic 유형은 CMV, yellow mosaic 유형은 AMV, severe mosaic 유형은 AzMV의 기주범위와 유사하였다. 4. 항혈청에 의한 각 병징별 반응결과 yellow mosaic 유형의 시요는 AMV 항 혈청과 mosaic 유형의 시요는 CMV의 항혈청과 침강선이 형성되어 각각 AMV 및 CMV의 한 계통으로 판정되었다. 5. 병징 유형별 전자현미경 관찰결과 yellow mosaic유형은 18~58 18nm의 타원형 입자가 다수 관찰되었고 mosaic 증상의 시요에서는 직경 30nm의 구형 입자, severe mosaic병징의 시요에서는 730 12nm의 사상형 입자와 봉입자가 관찰되었다.
        17.
        1995.10 KCI 등재 서비스 종료(열람 제한)
        충주팥의 전년산 바이러스 나병주로부터 얻은 종자가 차대 생육 및 수량에 미치는 영향을 구명하기 위하여 관행재배와 바이러스 매개충인 진딧물을 서단한 강실재배와 비교하고 감염된 바이러스의 형태를 관찰한 결과는 다음과 같다. 1. 매개충을 서단했던 강실재배에서는 전년도 나병주로부터 얻은 종자도 바이러스 감염증상을 발견할 수 없었으나 관행재배에서는 생육이 진전됨에 따라서 건전개체나 나병개체로부터 얻은 종자 공히 바이러스가 심하게 나타났다. 2. 관행재배에서는 바이러스 감염으로 인하여 생육이 부진하였으나 강실재배에서 는 심하게 나병된 개체에서 얻은 종자도 건전개체에서 얻은 종자와 같이 나병 주를 발견할 수 없었고 다소 도장되었으나 전반적으로 생육 및 수량성이 양호하였다. 3. 바이러스 감염이 없었던 강실재배는 관행재배에 비하여 협수, 분기수가 많았고 백립중도 무거웠으며 수량도 10a당 202kg으로서 210% 증수되었다. 4. 바이러스에 나염된 모자이크 증상의 엽조직을 50,000배의 전자현미경으로 검경한 결과 사상 모양의 바이러스 입자가 관찰되었다.