This study was established on which the selenium (Se), known as one of the functional elements in the human body, treatment was the most effective for the Se uptake in the soil and tree. Se treatments included foliar application, soil fertigation, and trunk injection. Se fertigation and control had similar soil P2O5, K, and Mg concentrations, and calcium and Se concentrations in the soil were greater on the control and Se fertigation, respectively. Leaf characteristics were not different among the treated trees. No differences were observed for the leaf K and Ca concentrations among the treated trees, and foliar Mg was greater on the Se treated trees than the control. Se foliar application and trunk injection had greater Se concentrations in the leaves and fruits than the Se fertigation and control.