Stomata are natural pores of plants and constitute the entry points for water during transpiration. However, they also facilitate the ingress of potentially harmful bacterial pathogens. The phytohormone abscisic acid (ABA) plays a pivotal role in protecting plants against biotic stress, by regulating stomatal closure. In the present study, we investigated the mechanism whereby ABA influences plant defense responses to Pseudomonas syringae pv. tomato (Pst) DC3000, which is a virulent bacterial pathogen of Arabidopsis, at the pre-invasive stage. We found that overexpression of two ABA receptors, namely, RCAR4/PYL10-OX and RCAR5/PYL11-OX (hereafter referred to as RCARs), resulted in ABA-hypersensitive phenotypes being exhibited during the seed germination and seedling growth stages. Sensitivity to ABA enhanced the resistance of RCAR4-OX and RCAR5-OX plants to Pst DC3000, through promoting stomatal closure leading to the development of resistance to this bacterial pathogen. Protein phosphatase HAB1 is an important component that is responsible for ABA signaling and which interacts with ABA receptors. We found that hab1 mutants exhibited enhanced resistance to Pst DC3000; moreover, similar to RCAR4-OX and RCAR5-OX plants, this enhanced resistance was correlated with stomatal closure. Taken together, our findings demonstrate that alteration of RCAR4- or RCAR5-HAB1 mediated ABA signaling influences resistance to bacterial pathogens via stomatal regulation.
유형이 다른 품종들간에 기공밀도와 기체확산저항의 차이와 광합성 속도에 미치는 영향 등을 비교 검토한 바 일반적으로 Indica 품종의 기공밀도가 Japonica 품종보다 컸으나 기체확산은 작았다. Indica 품종과 Japonica 품종간의 교잡을 통하여 육성한 품종들의 기공밀도와 기체확산 저항은 중간가를 나타냈다. 기공밀도와 기체확산저항 광합성 속도 간에는 현저한 정의 상관을 보였지만 특수엽중과는 현저한 질의 상관을 보였고, 단엽면적과의 상관관계는 현저하지 않았다. Indica 품종과 Japonica품종간의 교잡을 통하여 Indica 품종의 기공밀도가 크고 기체확산저항이 낮은 특성과 Japonica 품종의 특수 엽중이 큰 특성을 결합할 수 있다면 광합성 속도를 더욱 높일 수 있으리라 추측된다.
Diurnal changes in leaf stomatal resistances were measured on leaf positions and both surfaces to investigate the stomatal response to irradiance in wheat, var. Chokwang and barley, var. Dongbori 1. Stomatal frequency and size were also determined to explain the control mechanism of gas exchanges in two species. The leaf diffusive resistances of two species decreased, as the sun rose, to minimum at 10 to 11 o'clock a.m. and increased gradually in the afternoon, even faster at sunset. As the adaxial irradiance increased, stomatal resistances decreased sensitively in the range of 30uEm-2 ㆍsec-1 to 150uEm-2 ㆍsec-1 quantum flux density. The stomatal opening of the abaxial surface began at lower irradiance and was completed earlier than the adaxial surface. The adaxial irradiances decreased in order of leaf position, flag, the 2nd, the 3rd leaf, and the stomatal resistances increased in the same order. Even under the same irradiance, the stomatal resistance of lower leaves were higher than those of upper leaves. The stomatal frequencies of lower leaves were less, but the stomatal size was greater than those of upper leaves. Consequently, the relative leaf area occupied by stomatal pores were constant among leaf positions in two species.