The present study is designed to know the effect of cultivative environment and conditions, locational genetic variation on the content and components of seed isoflavones soybean genotype. The concentration of isoflavones showed difference between three different seeding time. Specially, Somyung of normal seeding (6. 21) revealed the highest concentrations (7657.0 ㎍ g-1) than other soybean seeds. On the other hand, Cheongjakong and Taekwangkong of early seeding (5. 25) showed the lower concentrations (1518.6 ㎍ g-1, 2524.0 ㎍ g-1) than other soybeans. For different regions, Taekwangkong of Yeoju region had the highest total isoflavone concentrations (3227.5㎍ g-1) than other cultivation regions. While, Taekwangkong of Miryang region (619.5 ㎍ g-1) revealed lower concentrations than other regions. And, Cheongjakong of Yeoju regions also revealed higher concentrations (3140.1 ㎍ g-1) than other regions. On the other hand, Cheongjakong of Miryang (1619.4 ㎍ g-1) showed lower concentrations of total isoflavone concentrations. In our present study, the isoflavone concentration of soybean was influenced by seeding and cultivation regions.