The purpose of this study is to develop transgenic cell line expressing targeted human granulocyte colony stimulating factor (hGCSF) and green fluorescence protein (GFP) genes as well as production of Somatic Cell Nuclear Transfer (SCNT) embryos derived from co-expressed transgenic donor cells. Constructed pPiggy-mWAP-hGCSF-EF1-GFP vector was chemically transfected into bovine fetus cells and then, only GFP expressed cells were selected as donor cells for SCNT. Cleavage and blastocyst rates of parthenogenetic, SCNT embryos using non-TG cell and hGCSF-GFP dual expressed SCNT embryos were examined (cleavage rate: 78.0±2.8 vs. 73.1±3.2 vs. 70.4±4.3%, developmental rate: 27.2 ±3.2 vs. 21.9±3.1 vs. 17.0±2.9%). Result indicated that cleavage and blastocyst rates of TG embryos were significantly lower (P<0.05) than those of parthenogenetic and non-TG embryos, respectively. In this study, we successfully produced hGCSF-GFP dual expressed SCNT embryos and cryopreserved to produce transgenic cattle for bioreactor system purpose. Further process of our research will transfer of transgenic embryos to recipients and production of hGCSF secreting cattle.

서 론
 재료 및 방법
  1. 소 미성숙난자의 회수 및 체외성숙 유도
  3. 유선조직 특이적인 hGCSF 유전자 발현을 위한 Vector의구축
  4. Vector 구축
  5. 소 성숙난자의 체세포 복제
  6. 소 복제수정란의 난활성 및 체외배양
  7. 형질전환 복제수정란의 RT-PCR 분석
  8. 통계분석
 결 과
 고 찰
 결 론
 REFERENCES

• 양정석(상지영서대학교 동물생명산업과,건국대학교 식품생명과학부) | Jung Seok Yang
• 조소영(상지영서대학교 동물생명산업과) | So Young Joe
• 구본철(대구가톨릭대학교 의학과) | Bon-Chul Koo
• 허영태(건국대학교 동물생명공학과) | Young-Tae Heo
• 강만종(전남대학교 축산학과) | Man-Jong Kang
• 송 혁(건국대학교 동물생명공학과) | Hyuk Song
• 이수민(상지영서대학교 동물생명산업과) | Su Min Lee
• 고대환(상지영서대학교 동물생명산업과) | Dae Hwan Ko
• 엄상준(상지영서대학교 동물생명산업과) | Sang Jun Uhm Correspondence