The purpose of this study was to observe the effects of the polysaccharide (GLP) obtained from the liquid cultured Ganoderma lucidum on the lipidperoxidation in a rat liver microsome and hepatotoxicity in the primary cultured rat hepatocytes. It is well known that the polysaccharide of Ganoderma lucidum exhibits hepatoprotective activity, antitumor activity etc., which many suggest a relationship to lipidperoxidation. The effect of GLP on CCl4- and galactosamineintoxicated cytotoxicity in the primary cultured rat hepatocytes were reduced the GPT value. In order to the estimate the effects of anti-lipidperoxidation of the polysaccharide, enzymatic and nonenzymatic reaction assays were performed, in vitro, in the rat liver microsome. An enzymatic lipidperoxidation reaction by ADP＋FeCl3＋NADPH and CCl4＋NADPH, GLP (1 mg/mL) inhibited 77.4% and 39.4%, respectively, and the nonenzymatic reaction displayed a 97.4% strongly inhibition. In the enzymatic and nonenzymatic inducers treated with GLP, the formation of malondialdehyde (MDA) progressively decreased by raising the GLP concentration. These results suggest that the anti-lipidperoxidation and radical scavenging activity of GLP may play an important part in the liver protection action.

Abstract
Introduction
Material and Method
    1. Materials
    2. Fermentation
    3. Preparation of GLP
    4. Isolation of hepatocytes
    5. Preparation of the microsomes
    6. Hepaprotective effect
    7. Measurement of lipidperoxidation
    8. Hydroxy radical scavenging activity
    9. Statistical analysis
Results
    1. Hepatoprotective activity of GLP
    2. Effect of GLP on ascorbate and FeSO4-induced LPO
    3. Effect of ADP＋FeCl3-induced LPO on GLP
    4. Effect of CCl4/NADPH-induced LPO on GLP
    5. Hydroxy radical scavenging activity
Discussion
References

• June-Woo Lee(Dept. of Hotel Culinary Art and Bakery, Kyungbuk College) Corresponding author