The root of Codonopsis lanceolata has been used in traditional medicine. This study was conducted to confirm the comparative effect of ethanol solvent extraction (CLE) and supercritical carbon dioxide extraction (CLS) of C. lanceolata roots. CLS had higher antioxidant than CLE. For supercritical co-solvent modified carbon dioxide extraction (CLS), it were extracted at 250 bar 50℃ 150 min at a flow rate of ethyl alcohol 3 mL/min for 90min. In addition, CLS inhibited the adipocyte differentiation of 3T3-L1 cells. When treated with the extract at a concentration of 100 μg/mL, the Wnt/β-catenin pathway reporter luciferase activity of HEK 293-TOP cells increased approximately by 3-folds compared to that of the untreated control group. Also, the treatment by CLS (50 μg/mL) showed a significant increase of involucrin expression. These results indicate that supercritical carbon dioxide extract of C. lanceolatamay serve as a cosmeceutical agent for improving skin barrier function and the treatment of obesity.

Abstract
1. Introduction
2. Experimental
    2.1. Sample preparation
    2.2. Cell culture and materials
    2.3. Cell viability
    2.4. Antioxidant assay
    2.5. Luciferase assay
    2.6. Adipocyte differentiation and Oil RedO staining
    2.7. Western blot and Immunoblotanalysis of 3D skin equivalent model
    2.8. Statistical analysis
3. Results and Discussion
    3.1. Effect of CL on cell viability
    3.2. Antioxidant effect of CL
    3.3. Effect of CLS on the adipocytedifferentiation and Wnt/β-cateninactivity
    3.4. Effect of CLS on involucrin expression
4. Conclusion
References

• Bora Kim(Division of Chemistry and Cosmetics, Mokwon University) Corresponding author