본 연구에서는 혈전용해능이 우수한 균주를 선별하여 청국장의 발효 특성을 비교 조사하였다. 시판청국장에서 분리된 5종 균주의 혈전용해능을 비교한 결과, N2가 (Plasmin lunit/mL과 비교)로 가장 높게 나타났고 N3, N5가 각각 를 나타내었다. N2 균주의 kit동정 결과, B. subtilis와 유사한 균주로 나타났다. 이를 활용하여 청국장을 제조한 결과, 분양균주에서는 B. subtilis (KCTC 3014)가 가장 많은 점질물

This study was carried out to investigate the Cheongbukjang fermentation characteristics and to select the strain showing a fibrinolytic activity. Fibrinolytic activity of 5 strains isolated from the commercial Chungkukjang was tested N2 strain showed the highest activity while N3 and N5 had similar activity compared to plasmin 1 unit/mL The selected N2 strain was determined as B. subtilis with homology by API kit analysis. Quality characteristics of Cheongbukjang fermented by 6 kinds of strains were tested Among 3 strains cultured, B. subtilis (KCTC 3014) showed the highest viscous substance, fibrinolytic activity and amino type nitrogen content. After isolated, B. subtilis N2 showed the highest viscous substance, fibrinolytic activity and amino type nitrogen content. Optimum steam-time for Cheongbukjang fermented by B. subtilis (KCTC 3014) and B. subtilis N2 was 45 min while optimum fermentation-time was 20 hr.

• 우승미
• 권중호
• 정용진