The ovicidal effect of plant extract mixture (5% cinnamon extract + 10% citronella oil + 30% citrus oil + 10% derris extract + 20% neem extract + 25% penetrating surfactant) against several major insect pests was tested using the spraying method. In the case of stink bugs, eggs tended to die during hatching. When treated with a plant extract mixture (500-times solution), mortality for Halyomorpha halys, Riptortus clavatus, Eurydema dominulus, Trialeurodes vaprarorium, Bemisia tabaci, Spodoptera exigua, and Agrotis ipsilon reached as high as 100%. Therefore, it is believed that industrialization will be feasible in the future.

To control western flower thrips (Frankliniella occidentalis), a significant pest of peppers, the control effects of eco-friendly agricultural materials were tested when treated alone and in combination. The control effects of yellow sticky roll traps installed on the stem, predatory natural enemy (Hypoaspis miles) inoculated on the stem, and plant extracts (50% custard apple seed oil + 10% cinnamon extract) sprayed on the foliage were 45.3%, 36.6%, and 50.2%, respectively, when treated individually. Meanwhile, when three eco-friendly agricultural materials were comprehensively applied, the control effect was maintained between 62.4% and 80.9% compared to untreated conditions during the pepper harvesting season. These methods can be utilized for the eco-friendly control of western flower thrips in pepper greenhouses.

Entomopathogenic fungi serve as eco-friendly alternatives to chemical pesticides. In this study, we investigate the interactions between mosquitoes and Metarhizium anisopliae JEF-157, which showed high insecticidal activity against mosquitoes, by RNA-seq analysis. RNA from mosquitoes was extracted at the median lethal time to identify changes in gene expression. The results showed 580 genes were up-regulated, while 336 genes were down-regulated in fungal treated mosquitoes. Up-regulated genes were related to metabolic and cellular processes such as cytochrome P450, DNA replication, and apoptosis. Down-regulated genes were involved in metabolism pathways such as lysosome, starch and sucrose metabolism, and fatty acid biosynthesis. These results are crucial for elucidating the mechanisms of fungal invasion and interaction in insects, providing insights for future pest management strategies.

The Republic of Korea (ROK), as a member state of the IAEA, is operating the State’s System of Accounting for and Control (SSAC) and conducting independent national inspections. Furthermore, an evaluation methodology for the material unaccounted for (MUF) is being developed in ROK to enhance capabilities of national inspection. Generally, physical and chemical changes of nuclear material are unavoidable due to the operating system and structure of facilities, an accumulation of material unaccounted for (MUF) has been issued. IAEA developed statistical MUF evaluation method that can be applied to all facilities around the world and it mainly focuses on the diversion detection of nuclear materials in facilities. However, in terms of the national safeguard inspection, an evaluation of accountancy in facilities is additionally needed. Therefore, in this research, a new approach to MUF evaluation is suggested, based on the Guide to the Expression of Uncertainty in Measurement (GUM) that an evaluation of measurement uncertainty factors is straightforward. A hypothetical list of inventory items (LII) which has 6,118 items at the beginning and end of the material balance period, along with 360 inflow and outflow nuclear material items at a virtual fuel fabrication plant was employed for both the conventional IAEA MUF evaluation method and the proposed GUM-based method. To calculate the measurement uncertainty, it was assumed that an electronic balance, gravimetry, and a thermal ionization mass spectrometer were used for a measurement of the mass, concentration, and enrichment of 235U, respectively. Additionally, it was considered that independent and correlated uncertainty factors were defined as random factors and systematic factors for the ease of uncertainty propagation by the GUM. The total MUF uncertainties of IAEA (σMUF) and GUM (uMUF) method were 37.951 and 36.692 kg, respectively, under the aforementioned assumptions. The difference is low, it was demonstrated that the GUM method is applicable to the MUF evaluation. The IAEA method demonstrated its applicability to all nuclear facilities, but its calculated errors exhibited low traceability due to its simplification. In contrast, the calculated uncertainty based on the GUM method exhibited high reliability and traceability, as it allows for individual management of measurement uncertainty based on the facility’s accounting information. Consequently, the application of the GUM approach could offer more benefits than the conventional IAEA method in cases of national safeguard inspections where factor analysis is required for MUF assessment.

본 연구에서는 기존 식품첨가물 분석법에서 합으로써 분 석되는 락색소를 laccaic acid A, B, C, E 4가지 성분으로 분류하고 개별적으로 정량 할 수 있는 분석법을 확립하였 다. Natural red 25를 사용하여 구조적으로 비슷한 laccaic acid A와 B를 1차적으로 분취한 후 2차로 A와 B를 분리 했다. 같은 방식으로 C와 D를 1차, 2차에 걸쳐 각각의 개 별 표준품으로 사용하였다. 락색소 불검출 시료 3가지 시료 (햄, 토마토 주스, 고추장)를 확보하여 0.05-107.2 μg/mL 범 위에서 결정계수(r2) 0.995 이상의 직선성을 확인하였다. 3 가지 시료에서 정밀도와 정확성을 측정한 결과, 일내 정 밀도는 0.2-12.3%, 정확도는 90.6-112.7% 범위 내에서 확인 되었으며 일간 정밀도는 0.3-13.3%, 정확도는 90.3-113.0% 범위내로 확인 되었다. 락색소를 사용하는 식품과 사용 금 지 식품에 대해 회수율을 측정한 결과, 사용 가능 식품에서 는 91.6-114.9% 범위의 회수율을 보였으며, 사용 불가 식품 의 경우 92.5-113.5% 범위의 회수율을 보였다. 락색소의 검 출 한계는 3가지 시료에서 검출한계 0.01-0.15 μg/mL, 정량 한계 0.02-0.47 μg/mL로 확인되었다. 락색소의 4가지 성분중 laccaic acid A와 C에 대한 측정 불확도를 산출한 결과, laccaic acid A의 측정 불확도는 13.65±0.39 mg/kg(신뢰수준 95%, K=2), laccaic acid C의 측정 불확도는 4.19±0.39 mg/kg(신뢰 수준 95%, K=2)로 비교적 낮은 측정불확도 값을 산출하 였다. 따라서 본 연구에서는 식품 중 락색소의 개별 분석 과 정성 및 정량분석을 위해 유효성이 검증된 분석법을 확립으로 식품 중 잔류물질 기준규격 설정 및 관리에 참 고 자료가 될 수 있고, 향후 매트릭스 효과에 따른 laccaic acid 개별 분석과 개별 활성 및 독성시험 연구의 근거 지 표가 될 수 있다고 판단된다.

한국의 목조건축문화유산은 상당수가 산림에 인접하여 흰개미에 의한 피해에 취약하다. 국내 흰개미 방제법은 살충제 접촉을 통한 화학적 방제법이 주로 이용되며, 방제 기간을 단축하기 위해서는 흰개미를 빠르게 약제까지 유인시킬 방법이 필요하다. 본 연구에서는 한국 서식 흰개미 에 대한 유인물질로 이산화탄소를 선정하여 유인여부 및 적절한 유인농도를 탐색하였으며, 지중 환경에서의 유인효력 기초평가를 진행하였다. 평가를 통해 흰개미에 대한 이산화탄소의 유인 효과를 확인하였으며, 이산화탄소 농도 10% 이하의 유효한 범위를 선정하였다. 또한 지중에서의 유인 효과를 확인함으로써 지중 환경에서의 적용 가능성을 파악하였다. 향후 현장에서 이산화탄소의 적용방안을 연구함으로써, 목조건축문화유 산의 흰개미 방제 시 개선된 방제 효과를 가질 수 있을 것으로 사료된다.