The yields of the n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insoluble fractions from the powdered water extracts were 8.2%, 10.6%, 32.0% and 49.2%, respectively (Table 1). The antifungal activities of water extracts, n-hexane-soluble fraction, ethyl acetate-soluble fraction, methanol-soluble fraction and methanol-insoluble fraction against L. edodes were 23.5%, 26.2%, 41.5%, 25.4% and 2.5%, respectively, at 1000ppm (Figure3). The ethyl acetate-soluble fraction showed much higher antifungal activity than the other fractions. The antifungal activity of the ethyl acetate-soluble fraction against L. edodes at 1000 ppm showed a statistically significant difference in the fractions of n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insolub