KOREASCHOLAR

현재까지 연구로는 흰목이 균사체에서 EPS 생산과 균사생장에 대한 적정 정치배양 조건이 연구되었다. 본 연구로부터 탄소원과 질소원의 처음 농도, 균사 형태와 발효조의 타입의 선택은 흰목이 균사체 EPS 생산에 가장 영향을 미친다는 것을 알게 되었다. 이들 결과는 공기주입식 반응기에서 EPS 생산성은 진탕탱크 반응기 보다 더 높았다는 점을 증명하였다. 또한 흰목이 균사의 정치배양의 생리적 생장에 대한 지식은 아직도 제한적이다.

The optimization of submerged culture conditions for mycelial growth and exopolysaccharide (EPS) production in an edible mushroom Tremella fuciformis were studied in shake flasks and bioreactors. The temperature of 28℃ and pH 8 in the beginning of fermentation in agitated flasks was the most efficient condition to obtain maximum mycelial biomass and EPS. The optimal medium constituents were as follows (g l-1): glucose 20, tryptone 2, KH2PO4 0.46, K2HPO4 1 and MgSO4H2O 0.5. The fungus was cultivated under various agitation and aeration conditions in a 5L stirredtank bioreactor. The maximum cell mass and EPS production were obtained at a relatively high agitation speed of 200 rpm and at an aeration rate of 2 vvm. The flow behavior of the fermentation broth was Newtonian and the maximum apparent viscosity (35 cP) was observed at a highly aerated condition (2 vvm). The EPS productivity in an airlift reactor was higher than that in the stirred-tank reactor. The EPS was protein-bound polysaccharides consisted of mainly mannose, xylose, and fructose. The molecular weights of EPS were determined to be 1.3~1.5×106.

ABSTRACT
 서론
 재료 및 방법
  미생물 및 배지
  접종원 제조 및 플라스크 배양
  생물반응 발효조
  셀 건조중 및 EPS 농도
  아미노산과 탄수화물의 분석
  영상분석 순서
  EPS(exopolysaccharide)의 특성 평가
 결과 및 고찰
  플라스크 배양에서 최초 산도와 온도의 영향
  플라스크 배양의 적정한 배지구성 조합
  진탕 탱크배양 반응기의 진탕속도의 영향
 적요
 감사의 글
 인용문헌

• 장현유(한국농업대학) | Hyun- You Chang
• 이찬( 중앙대학교) | 이찬
• 최성우( 수원대학교) | 최성우
• 윤정원( 대구대학교) | 윤정원