KOREASCHOLAR

Characterization of intraspecific hybrids of Pleurotus ostreatus based on mitochondrial DNA profiles of di -monokaryon and mono-monokaryon crosses

Eun Jung Kim, Won Sik Kong, Kab Yeul Jang, Pyung Gyun Sin, Yunjung Park, Young sook Hahn, Young Bok Yoo
  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/276354
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한국버섯학회지
제8권 제4호 (2010.12)
p.181
한국버섯학회 (The Korean Society of Mushroom Science)
초록

This study was conducted to develop superior hybrids of Pleurotus ostreatus with di-mono and mono-monoka-ryon crosses. Random Amplified Polymorphic DNA-PCR (RAPD-PCR) was used to compare its mitochondrial DNA profiles of hybrids using specific primers designed from microsatellite markers of Pleurotus salmoneo-stramineus. A total of fifty-six dikaryon-monokaryon hybrids were sampled for RAPD-PCR experiments and the results show that twenty-four hybrids were dikaryon and thirty-one hybrids were monokaryon. Interestingly, one hybrid was an intermediate form with the DNA profiles that are different from those of its parents. The DNA profiles from eighty-eight monokaryon-monokaryon hybrids were also analyzed by RAPD-PCR. The results of mitochondria DNA profiles show that seventy-one hybrids are the same to one of their parents, but seventeen hybrids show DNA profiles of both parents.

키워드
저자
  • Eun Jung Kim(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, 1Suwon 441-707, Korea. Department of food and nutrition, Sungshin women’s university)
  • Won Sik Kong( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, 1Suwon 441-707, Korea. Department of food and nutrition, Sungshin women’s university) | Won Sik Kong
  • Kab Yeul Jang( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, 1Suwon 441-707, Korea. Department of food and nutrition, Sungshin women’s university) | Kab Yeul Jang
  • Pyung Gyun Sin( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, 1Suwon 441-707, Korea. Department of food and nutrition, Sungshin women’s university) | Pyung Gyun Sin
  • Yunjung Park( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, 1Suwon 441-707, Korea. Department of food and nutrition, Sungshin women’s university) | Yunjung Park
  • Young sook Hahn( Suwon 441-707, Korea. Department of food and nutrition, Sungshin women’s university) | Young sook Hahn
  • Young Bok Yoo( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA, 1Suwon 441-707, Korea. Department of food and nutrition, Sungshin women’s university) | Young Bok Yoo