The price of mushrooms harvested by bottle cultivation is rapidly dropping and the income of farm households is also rapidly decreasing due to the increase of production cost. Although some of these mushroom farms have employed the systems of mass production, they are in financial difficulty because of the investment they need to do for facilities such as cultivation room and automated systems. In the other hands, some retailers want to buy a small volume of mushrooms of many different mushrooms produced by the small farms and these small farms was required to investigate the common cultural condition for mushroom production of many different mushrooms. In this study, we investigated the common cultural conditions for production of many different mushrooms (e.g., Pleurotus eryngii and Agrocybe cylindracea) at the bottle cultivation farms. The cultural period of Pleurotus eryngii and Agrocybe cylindracea was 30~35 days. The optimum temperature of the mycelial growth was 25~28℃ with the growth room being maintained about 20~23℃ with the consideration of the respiration heat of the mycelium. The temperature of mushroom growth room was 16~18℃ for all growth periods. In our results, Pleurotus eryngii and Agrocybe cylindracea produced the highest yields at the substrate formulation of sawdust 75%, rice brain 20%, soybean cake wastes 5%, water contents 70% and 850 ml P.P. In the long run, our results will result in the development of new automatic cultivation model and increase the income of small production farms.