KOREASCHOLAR

Oyster mushrooms including of P. ostreatus, P. eryngii, P. pulmonarius and P. cornucopiae are one of the famous mushrooms for foods in Korea. RAPD were carried out using 14 of oligoprimers to analyze the phylogenetic relationship among 57 strains of 32 Pleurotus species. Most of species formed the minimum clade with strains within species and was divided respectively species. Therefore clade was separated well in accordance species. Pleurotus species formed again clade to be added in close related to other species, and were discriminated by sixteen clades with each representative species including high similarity groups. Sixteen clades were composed representative species according to each clade. There were clade I of P. pulmonarius(P. sajor-caju, P. opuntiae, P. sapidus), clade II of P. eryngii(P. fuscus var. ferulae, P. fossulatus), clade III of P. ostreatus(P. ostreatus var. columbinus, P. spodoleucus, P. floridanus), clade IV of P. florida, clade V of P. djamor(P. flabellatus, P. incarnates, P. salmoneo-stramineus), clade VII of P. populinus(P. subareolatus), clade VIII of P. cystidiosus(P. cystidiosus var. formosensis), clade X of P. dryinus(P. dryinus var. pometi), clade XIV of P. cornucopiae(P. citrinopilieatus, P. euosmus), and clade XV of P. australis. These species were representative species each clades. Five species, P. ulmarius(clade VI), P. griseus(clade IX), P. calyptratus(clade XI), P. lampas(clade XII), P. smithii(clade XIII)and P. serotinus (clade XVI) were used each one strain in analysis, so they were clustered other groups.

 ABSTRACT  Introduction  Material and Methods   Fungal Strains   Sample Preparation and Genomic DNA Extraction   Amplification of RAPD using arbitrary primers   Phylogenetic analysis of DNA amplificationfingerprints  Results and Discussion  Acknowledgements  References

• Choi, Sun-Gyu(Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
• Jang, Kab-Yeul( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Jang, Kab-Yeul
• Kim, Gyu-Hyun( Department of Biotechnology, Yonam college of Agriculture) | Kim, Gyu-Hyun
• Kong, Won-Sik( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Kong, Won-Sik
• Jo, Jae-Sun( Department of Food Engineering, Kyung Hee University) | Jo, Jae-Sun
• Kim, Hae-Yeong( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Kim, Hae-Yeong
• Yoo, Young-Bok( Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) | Yoo, Young-Bok