‘Nongjin-go’ is a new breed strain of Lentinula edodes, saw-dust bag variety. It is a cross combination of dikaryonLentinula edodes ASI 3305mut and monokaryon L5-16 of L. edodes ASI 3305(Sanjo701ho). We crossbred them by 2011 andverified productive capacity from 2012 to 2013 in Rural Development Administration. Optimum temperature of mycelial growthis 30oC and it of fruit-body primodium formation is range from 15 to 23oC. Nongjigo is agricultured at mid-high temperaturewell. Fruiting body is platy-hemisphere, light brown and centralizing. And bast is formed around edge of pileus. Yield productionsper period is regular than ‘Sanjo701’. Plastic bag culture medium is 1.5 kilogram and culture periods are 90~100 days. As itsbrowning of pileus in culture is a little slow, Light and ventilation is needed a lot in light-culturing. Humidity is controlled properlyfor its color in fruit-body growing. Tested culture medium is consisted of 80% Oak-Tree saw-dust and 20% rice-bran.