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Inter simple sequence repeat (ISSR)-PCR에 의한 양송이버섯(Agaricus bisporus) 계통과 단핵균주의 다형성 분석 Inter simple sequence repeat (ISSR)-PCR based polymorphism of Agaricus bisporus strains and monokayon isolates

민경진, 공원식, 강희완
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  • URLhttps://db.koreascholar.com/Article/Detail/307740
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한국버섯학회지
제13권 제3호 (2015.09)
pp.175-180
한국버섯학회 (The Korean Society of Mushroom Science)
초록

Twenty Inter simple sequence repeat (ISSR) primers were used to assess genetic diversity of 64 Agaricus strainsincluding 45 A. bisporus strains and other 19 Agaricus spp. ISSR primers, (GA)T, (AG)YC, (GA)C and (CTC) amplified PCRpolymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted forUPGMA cluster analysis. The varieties, Saea, Saedo, Saejeong and Saeyeon that have recently been developed in Korea wereinvolved in the same group with closely genetic relationship of coefficient similarity over 0.92, whereas, other Korean strains weregenetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese. Furthermore, ISSR-PCRpolymorphism could potentially be used to identify homokaryon isolates.

키워드
Agaricus bisporus strainsGenetic diversityISSRPCR polymorphism
목차
서 론
 재료 및 방법
  공시균주 및 배양
  Genomic DNA 추출
  PCR 다형성 분석
  유전적 유연관계 분석
 결과 및 고찰
  양송이 계통간 ISSR-PCR 다형성 및 유연관계분석
  ISSR 마커에의한 단포자 PCR다형성 분석
 적 요
 감사의 글
 References
저자
  • 민경진(한경대학교 미래융합기술대학원, ㈜ 제이케이바이오테크) | Kyong-Jin Min
  • 공원식(농촌진흥청 국립특작과학원 버섯과) | Won-Sik Kong
  • 강희완(한경대학교 미래융합기술대학원, ㈜ 제이케이바이오테크3농촌진흥청 국립특작과학, 한경대학교 유전공학연구소) | Hee-Wan Kang *Corresponding author