양송이배지로부터 분리한 Burkholderia균의 인산가용화 공조효과 Synergistic effect of phosphate solubilization by Burkholderia strains isolated from button mushroom bed
pp.183-189
This study investigated the synergistic effect of single inoculation and co-inoculation of phosphate-solubilizing bacteria (PSB) Burkholderia metallica JH-7 and Burkholderia contaminans JH-15. Phosphate-solubilizing abilities of these strains were assessed by measuring phosphorus content in culture media that were singly inoculated or co-inoculated with these strains for 7 days. B. metallica JH-7 was found to release the highest content of soluble phosphorus (140.80 μg mL-1) into the medium, followed by single inoculation of B. contaminans JH-15 (135.95 μg mL-1) and co-inoculation of two strains (134.84 μg mL-1). The highest pH reduction, organic acid production, and glucose consumption were observed in the medium inoculated with B. metallica JH-7 alone compared with that in the medium co-inoculated with both the strains. Results of a plant growth promotion bioassay showed 17.4% and 7.48% higher leaf and root growth, respectively, in romaine lettuce inoculated with B. metallica JH-7 alone than in romaine lettuce inoculated with a control strain. However, no significant difference was observed between single inoculation and co-inoculation of these strains with respect to phosphorus release and plant growth. Although the results of the present study did not show the synergistic effect of phosphate solubilization by the PSB strains examined, these results indicate that treatment with PSB exerts a beneficial effect on crop growth.
재료 및 방법
인산가용화균의 분리
미생물 배양
가용화인의 함량분석
배지의 pH 및 잔류당 분석
HPLC에 의한 유기산 분석
상추재배실험
결과 및 고찰
분리균의 동정
가용화 인의 함량
배지 내 pH의 변화
배지 내 잔류당 변화
HPLC에 의한 유기산 분석
상추생육효과
적 요
감사의 글
References