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질산납이 한 국산 무미양서류의 배아발달에 미치는 영향 The Effects of Lead (II) Nitrate on the Embryo Development in Native Amphibians

이해범, 고선근
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  • URLhttps://db.koreascholar.com/Article/Detail/339305
Korean Journal of Environmental Biology (환경생물)
Vol. 35 No. 4 (2017.12)
pp.706-714
한국환경생물학회 (Korean Society Of Environmental Biology)
초록

An investigation of the effects of Pb for domestic anuran embryos were evaluated with the Frog Embryo Teratogenesis Assay; Xenopus (FETAX). Depending on the species, the difference between the embryo size and the embryonic development time was determined. As a result, mortality and malformation rates were increased, malformation patterns were changed and larval body length were decreased in a dose dependent manner of the Pb. The half maximal lethal concentration (LC50) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.58, 0.49, 0.52, 0.54 mg L-1, respectively. The half maximal effective concentration (EC50) of the Bufo gargarizans, Hyla japonica, Rana nigromaculata and Bombina orientalis were 0.35, 0.74, 0.30, 0.29 mg L-1, respectively. The teratogenic index (TI) were 1.66 in the Bufo gargarizans, 1.81 in the Hyla japonica, 1.73 in the Rana nigromaculata and 1.86 in the Bombina orientalis, respectively. Therefore, the Pb seems likely to have a teratogenic effect in all four species’ embryonic development. The Bombina orientalis was the most sensitive to the Pb. This means that the difference between the different species, even if they have all been exposed to the same concentration of pollutants depending on the species. The result above show that the Pb acts as a teratogenic agent in the development of the four domestic frog species.

목차
서 론
 재료 및 방 법
  1. 실험동물과 배아 준비
  2. 시험물질 처리
  3. 결과 분석
 결 과
  1. 정상발생과정 조사
  2. Pb의 농도에 따른 치사율 조사
  3. Pb 농도에 따른 기형율 조사
  4. Pb 농도에 따른 기형 양상 조사
  5. Pb 농도에 따른 성장률 조사
  6. Pb에 의한 기형성 지수 조사
 고 찰
 적 요
 REFERENCES
저자
  • 이해범(전남대학교 대학원 생물과학·생명기술학과, Graduate School of Biological Sciences and Biotechnology, Chonnam University) | Hae-Bum Lee
  • 고선근(호남대학교 물리치료학과, Department of Physical Therapy, Honam University) | Sun-Kun Ko Corresponding author