꽃송이버섯 안정생산을 위해 액체종균배지조성 및 배양조건에 관한 연구결과, 물엿을 8Brix%로 조절하고 질소원으로 Yeast extract를 0.2%첨가한 배지에서 균사생장량이 우수하여 적합 배지로 선발하였다. 통기량에 따른 CO2발생량은 배양 9일까지 증가하다 감소하였고, 통기량이 높을수록 균체직경은 작아지고 배지감소율은 증가하여 통기량 0.9vvm이상에서 배지감소율이 급증하여 꽃송이버섯 액체종균에 적합한 통기량은 0.3~0.6 vvm 이 적합하였다. 또한, 배양기간일 길수록 균체량이 많았으나, CO2발생량이 높고 배지감소율이 낮은 9~11일을 적합 배양기간으로 설정하였다.
Sparassis latifolia is one of the most expensive mushrooms in Korean market owing to its high β-glucan content and immunoactivity. However, because of the long cultivation period and high contamination rates, it has low production efficiency. Therefore, we first need to establish the optimum conditions for liquid spawn production to increase its production efficiency. As a result of experiments, molasses culture medium was selected for mycelial growth. Also, the optimum sugar content for molasses and amount of aeration used were approximately 8 Brix% and 0.3~0.6 vvm, respectively. Mycelial dry weight increases, while the medium decreases, as the incubation period increases. Therefore, to achieve maximum production efficiency, the incubation period of 9 to 11 days is appropriate.