KOREASCHOLAR

PC12 신경세포주에서 녹각영지버섯 주정 추출물의 산화 스트레스 개선 효과 Protective effect of ethanolic extract of antler-shaped Ganoderma lingzhi against oxidative stress in PC12 neuronal cell line

김형돈, 이은영, 박정용, 서경혜, 이강효, 최재훈, 한재구, 조재한, 이승은
  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/357317
PDF 원문보기
한국버섯학회지
제16권 제3호 (2018.09)
pp.213-217
한국버섯학회 (The Korean Society of Mushroom Science)
초록

This study was carried out to identify medicinal mushrooms with protective effects against oxidative stress in PC12 neuronal cell line, followed by evaluation of their antioxidant property. Extracts of medicinal mushrooms, including Ganoderma lucidum extract (GLE), antler-shaped Ganoderma lingzhi extract (AGLE), Hericium erinaceus extract (HEE), and Sanghuangporus baumii extract (SBE), were screened for cytotoxicity using MTT assay. None of the extracts up to 10 μg/ml concentration affected cell viability. These extracts were further checked for their protective effect against oxidative stress-induced reactive oxygen species (ROS) production. Exposure to 50 μM H₂O₂ induced ROS generation in PC12 cells, which was inhibited only by treatment with AGLE. In addition, inhibition of H₂O₂-induced ROS generation by AGLE was found to be in a dose-dependent manner (2.5, 5, and 10 μg/ml). Microscopic examination of DCF fluorescence for detection of ROS showed a similar pattern. Further, antioxidant activity of AGLE was determined by ABTS radical cation assay, and its IC50 was found to be 46.90±0.31 μg/ml. Taken together, these results suggest that AGLE may help to alleviate oxidative stress in PC12 neuronal cells.

키워드
Antler-shaped Ganoderma lingzhiNeuronal cellOxidative stress
목차
ABSTRACT
 서 론
 재료 및 방법
  공시균주
  버섯 추출물 제조
  세포주 배양
  세포 생존율 측정
  활성산소 (reactive oxygen species, ROS) 생성량 측정
  2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonicacid)(ABTS) 라디칼 소거능 측정
  형광현미경 관찰
  통계처리
 결과 및 고찰
  신경세포주에서 여러 가지 약용 버섯 추출물의 처리농도 결정
  신경세포주에서 산화 스트레스 개선 약용 버섯 추출물탐색
  녹각영지버섯 추출물의 ABTS 라디칼 소거능 측정
  신경세포주에서 녹각영지버섯의 산화 스트레스 개선 효과
 적 요
 REFERENCES
저자
  • 김형돈(농촌진흥청 국립원예특작과학원 인삼특작부) | Hyung Don Kim (Department of Herbal Crop Research, NIHHS, RDA)
  • 이은영(농촌진흥청 국립원예특작과학원 인삼특작부) | Eun Young Lee (Department of Herbal Crop Research, NIHHS, RDA)
  • 박정용(농촌진흥청 국립원예특작과학원 인삼특작부) | Jeong-Yong Park (Department of Herbal Crop Research, NIHHS, RDA)
  • 서경혜(농촌진흥청 국립원예특작과학원 인삼특작부) | Kyung Hye Seo (Department of Herbal Crop Research, NIHHS, RDA)
  • 이강효(농촌진흥청 국립원예특작과학원 인삼특작부) | Kang-Hyo Lee (Department of Herbal Crop Research, NIHHS, RDA)
  • 최재훈(농촌진흥청 국립원예특작과학원 인삼특작부) | Jehun Choi (Department of Herbal Crop Research, NIHHS, RDA)
  • 한재구(농촌진흥청 국립원예특작과학원 인삼특작부) | Jae-Gu Han (Department of Herbal Crop Research, NIHHS, RDA)
  • 조재한(농촌진흥청 국립원예특작과학원 인삼특작부) | Jae-Han Cho (Department of Herbal Crop Research, NIHHS, RDA) Corresponding author
  • 이승은(농촌진흥청 국립원예특작과학원 인삼특작부) | Seung Eun Lee (Department of Herbal Crop Research, NIHHS, RDA) Corresponding author