KOREASCHOLAR

참나무 단목원목으로 재배한 P. linteus KACC93057P (PLHS)2년산 자실체 60%에탄올 추출물의 총 페놀함량은 19.05±0.32 mg GAE/g로 다른 비교구 버섯 종에 비하여 4배에서 10배이상의 높게 나타났다. 125 μg/mL농도에서 DPPH와 ABTS 라디칼 소거능은 62%와 100%를 나타냈으며 FRAP 환원력은 0.7μMFeSO4/g 으로 비교 구 버섯종 보다 2에서 10배이상의 유효활성이 검출 되었다. PLHS 자실체 ethyl acetate분배 추출물의 HPLC분석에서 styrylpyrone 계열의 화합물 davallialactone, hispidin, hypholomine B와 phenylpropanoid 계열의 화합물인 caffeic acid가 동정 되었다.

The aim of this study was to identify chemical composition and antioxidant activity of phenolic extract of fruiting bodies of an artificially cultivated Hankyong Sanghwang mushroom, Phellinus linteus KACC 93057P (PLHS). The total phenolic content of 60% ethanolic extract of fruiting bodies of two-year-old PLHS grown on Oak wood logs was 19.05±0.32 mg gallic acid equivalent (GAE)/g, which was 4-10 times high compared to the other species of mushrooms. 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbisthiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and the ferric reducing antioxidant power (FRAP) value of PLHS were 2-10 and 5 times higher, respectively, than those of the other species of mushrooms. High performance liquid chromatography (HPLC) analysis of ethyl acetate fraction of 70% methanolic extract of the fruiting bodies of PLHS revealed the presence of styrylpyrone-class compounds, davallialactone, hispidin, hypholomine B, and caffeic acid, a compound of the phenylpropanoid pathway.

ABSTRACT
 서 론
 재료 및 방법
  균주배양 및 종균
  상황버섯인공재배
  항산화활성 페놀성분 추출
  총 폴리페놀 함량 측정
  DPPH 라디칼 소거능 활성 측정
  ABTS 라디칼 소거능 활성 측정
  FRAP를 이용한 총 항산화력 측정
  통계처리
  페놀화합물 동정
 결과 및 고찰
  목질진흙버섯 KACC93057P의 인공재배 자실체의 형태적 특성
  총 페놀함량
  항산화활성
  페놀화합물의 동정
 적 요
 REFERENCES

• 민경진(한경대학교 미래융합기술대학원) | Gyong-Jin Min (Graduate School of Future Convergence Technology, Hankyong National University)
• 정이은(한경대학교 미래융합기술대학원) | Ee-un Jeong (Graduate School of Future Convergence Technology, Hankyong National University)
• 윤봉식(전북대학교 생명공학부) | Bong-Sik Yun (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
• 강희완(한경대학교 미래융합기술대학원, 한경대학교 원예생명과학과, 한경대학교유전공학연구소) | Hee-Wan Kang (Graduate School of Future Convergence Technology, Hankyong National University, Department of Horticultural Life Science, Hankyong National University, Institute of Genetic Engineering, Hankyong National University) Corresponding author