KOREASCHOLAR

An auxin-producing bacterium Yangsong-1 was isolated from a button mushroom bed in Chung cheongnam-do. The strain Yangsong-1 was classified as a novel strain of Arthrobacter enclensis based on a chemotaxonomic and phylogenetic analysis. The isolated A. enclensis Yangsong-1 was confirmed to produce indole-3-acetic acid (IAA), which is one of the auxin hormones. When the concentration of IAA was assessed by HPLC quantity analysis, the maximum concentration of IAA, 152.903 mg L-1, was detected from the culture broth incubated in R2A medium containing 0.2% L-tryptophan for 48 h at 35oC. A negative relationship between IAA production and pH was estimated to show that the increase in IAA caused pH acidification of the culture. The effect of the supplement on L-tryptophan, a known precursor of IAA production, appeared to be at maximal production at 0.2% concentration and was rather reduced at concentration above 0.4%. To investigate the growth-promoting effects on the crops, the culture broth of A. enclensis Yangsong-1 was placed in water cultures and seed pots of mung beans and lettuce. In consequence, the adventitious root induction and root growth of mung beans and lettuce were 1.5 and 1.9 times higher, respectively, than those of the control.

ABSTRACT
 서 론
 재료 및 방법
  균주의 분리
  Auxin 생성균주의 선발
  TLC에 의한 IAA의 확인
  HPLC에 의한 IAA의 정량분석
  Bio assay를 통한 식물생장실험
   가. 녹두발근생검법 (수경법)
   나. 녹두발근생검법 (Pot실험)
   다. 상추발근생검법 (Pot실험)
   분리 균주의 동정
 결과 및 고찰
  균주의 분리
  분리균의 동정
  Auxin 물질의 확인 및 생산능 비교
  Bio assay를 통한 식물생장 실험
  수경법에 의한 녹두발근력 확인실험
  Pot실험에 의한 녹두발근생검법
  Pot실험에 의한 상추발근생검법
 적 요
 References

• 문서진(충남대학교 농업생명과학대학 생물환경화학과) | Seo-Jin Moon (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
• 윤민호(충남대학교 농업생명과학대학 생물환경화학과) | Min-Ho Yoon (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) Corresponding author