KOREASCHOLAR

Sparassis latifolia is a fungus abundant in β-glucan and amino acids and is highly valued as a medicinal mushroom. Among amino acids, γ-aminobutyric acid (GABA) is a free amino acid and has biological effects, such as increase/decrease of hypertension, improvement of cerebral blood flow, and prevention of dementia. In this study, biological elicitors were used to increase bioactive substances as a biofortification method. Sodium alginate extracted from seaweed (Sargassum horneri, Sargassum fulvellum, Sargassum fusiforme) were used as the elicitor. The levels of β-glucan and GABA in the mycelium and fruiting body grown by adding the elicitor to the medium were investigated. Addition of sodium alginate positively affected GABA production and negatively affected the β-glucan production in these fungi. Sodium alginates extracted from S. fulvellum induced the highest increase in GABA in the mycelium and fruiting bodies. Moreover, we investigated the effects of the extracts from mycelium and fruiting bodies on dendrite development in primary cortical neurons. We found that the extract from the fruiting bodies of sodium alginate treated fungi with increased levels of GABA inhibited the dendrite outgrowth of excitatory neurons, but not inhibitory neurons.

ABSTRACT
서 론
재료 및 방법
    꽃송이버섯 공시균주 및 생장
    해조류 기반 엘리시터 제조
    β-Glucan 측정
    GABA 측정
    초대 신경세포 배양
    면역형광법
    MTT assay
    Western blotting
    Dendrite outgrowth 측정
    통계
결과 및 고찰
    β-Glucan 측정결과
    GABA 측정결과
    독성실험결과
    초대 신경세포 수상돌기 발달 결과
    신경세포 내 신호전달경로의 변화결과
결 론
REFERENCES

• 최문희(조선대학교 공학기술연구원) | Moon-Hee Choi (The Research Institute of Advanced Engineering Technology, Chosun University)
• 기성환(조선대학교 약학대학) | SungHwan Ki (College of Pharmacy, Chosun University)
• 이성은(조선대학교 약학대학) | Seong-Eun Lee (College of Pharmacy, Chosun University)
• 이금화(조선대학교 약학대학) | GumHwa Lee (College of Pharmacy, Chosun University)
• 신현재(조선대학교 생명화학고분자공학과) | Hyun-Jae Shin (Department of Biochemical Polymer Engineering of Chosun University) Corresponding author