KOREASCHOLAR

Optimization of medium components and incubation time for the production of Paecilomyces tenuipes mycelia in submerged culture

Si Young Ha, Ji Young Jung, Dong Hwan Lee, Jae-Kyung Yang
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  • URLhttps://db.koreascholar.com/Article/Detail/405909
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한국버섯학회지
제19권 제1호 (2021.03)
pp.1-8
한국버섯학회 (The Korean Society of Mushroom Science)
초록

The choice of the culture medium is an important factor for the mass production of mycelia in submerged cultures. The influence of liquid medium on the mycelial dry weight of Paecilomyces tenuipes was investigated in this study. The regression equation is expressed as Y=-1292.94187+17.78612X1+18.92425X2+2.11464X3-0.019375X1X2-0.006276X1X3+0.008177X2X3- 0.070169X1 2-0.292175X2 2-0.008818X3 2, where Y represents the value of the mycelial dry weight (g/L), X1 is the particle size of wood sawdust in liquid medium (mesh), X2 is the concentration of the wood sawdust in liquid medium, and X3 is incubation time (h). The medium was optimized using a response surface methodology, and the optimal medium contained 30 g of wood sawdust (140 mesh), 20 g of glucose, and 10 g/L of peptone. Under these conditions, the mycelial dry weight reached 38.1 g/L (actual value). The culture medium containing wood sawdust is simple and easy to use, highly efficient, and eco-friendly, and its effectiveness in large preparations of P. tenuipes mycelia with low material costs has been demonstrated.

키워드
MediumMyceliumPaecilomyces tenuipesResponse surface methodologySubmerged culture
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
    Maintenance and seed culture
    Preparation of liquid medium and components
    Determination of mycelial dry weight
    Experimental design by Box-Behnken design (BBD) ofresponse surface methodology (RSM) for optimization ofliquid medium composition and incubation time
    Determination of cordycepin content by highperformance liquid chromatography
    Statistical analysis
RESULTS AND DISCUSSION
    Effect of different liquid media on mycelial dry weight
    Optimization of screened variables using RSM(BBD) for mycelia growth
REFERENCES
저자
  • Si Young Ha(Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University)
  • Ji Young Jung(Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University)
  • Dong Hwan Lee(Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University)
  • Jae-Kyung Yang(Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University) Corresponding author