Optimization of Monochamus alternatus media and culture period for cordycepin production in Cordyceps militaris culture using solid-state fermentation
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/409784
pp.126-133
In this study, we investigated the effect of solid culture medium on the production of cordycepin in Cordyceps militaris. The regression equation was expressed as follows: Y1= 755.3-58.6625X1+4.79432E-14X2-46.6625X3-5.66269E-14X1X2- 0.025X1X3+1.62475E-14X2X3-160.6625X1 2+0.0125X2 2-206.9625X3 2, where, Y represents the value of cordycepin content (μg/g), X1 corresponds to the weight of M. alternatus in solid culture medium (g/bottle), X2 to the water content of the solid culture medium (%), and X3 to the culture period (day). The solid culture medium was optimized using the response surface methodology, and the optimal medium composition was as follows: the weight of M. alternatus in solid culture medium and water content were 16.2% and 100.7% (20.14 mL water/20 g solid culture medium), respectively, with a culture period of 39 days. Under these conditions, the cordycepin content of the fruiting bodies reached 150.0 μg/g (actual value). The supplementation of M. alternatus in solid culture for improved cordycepin content of C. militaris seems to be a promising alternative to wild and solid cultivation.
INTRODUCTION
MATERIALS AND METHODS
Fungal strains
Inoculum preparation
Solid-state fermentation for growth of fruiting body
Analytical methods
Statistical analysis
RESULTS AND DISCUSSION
Influence of M. alternatus form on cordycepin content offruiting bodies
Effect of M. alternatus supplements weight on cordycepincontent of fruiting body
Effect of solid culture medium water content oncordycepin content of fruiting body
Optimization of screened variables using RSM (BBD)for improved cordycepin content of fruiting body
REFERENCES