KOREASCHOLAR

The demand for novel strains has been rising in the domestic market to increase the production of sclerotia from Wolfiporia hoelen. To improve strain breeding efficiency, we investigated whether single-nucleotide polymorphisms (SNPs) in the RNA polymerase II subunit (RPB2) gene, which may be linked to the mating type locus, are useful for distinguishing monokaryons from dikaryons in Korean W. hoelen strains. We designed a specific primer set to efficiently amplify a region of RPB2 using PCR with the genomic DNA of 12 cultivated strains and 31 wild strains of W. hoelen collected from Korea. Nucleotide sequences of the PCR-amplified RPB2 genes were determined and analyzed for the presence of SNPs among the 43 W. hoelen strains. Previously reported SNP loci were detected in the RPB2 gene of all W. hoelen strains tested. However, these previously reported SNP loci could not be applied to differentiate monokaryons from dikaryons in approximately one-third of Korean wild strains with homozygous genotypes. Three additional SNPs in the RPB2 gene, which may improve the ability to distinguish monokaryons from dikaryons, were identified by searching through the multiple sequence alignments of the 43 W. hoelen strains. The applicability of these three novel SNPs, together with the previously known SNPs, in the RPB2 gene to W. hoelen strain breeding was verified by examining the hybrid strains and their parental strains.

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REFERENCES

• 김수연(국립산림과학원 산림생명자원연구부 산림미생물연구과, 단국대학교 과학기술대학 미생물학과) | Su Yeon Kim (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science, Department of Microbiology, College of Science & Technology, Dankook University)
• 박미정(국립산림과학원 산림생명자원연구부 산림미생물연구과) | Mi-Jeong Park (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science, Suwon 16631, Republic of Korea)
• 김성환(단국대학교 과학기술대학 미생물학과) | Seong Hwan Kim (Korea 2Department of Microbiology, College of Science & Technology, Dankook University, Cheonan 31116, Republic of Korea)
• 가강현(국립산림과학원 산림생명자원연구부 산림미생물연구과) | Kang-Hyeon Ka (Forest Microbiology Division, Department of Forest Bio-Resources, National Institute of Forest Science, Suwon 16631, Republic of Korea) Corresponding author