KOREASCHOLAR

In this study, we aimed to compare the mycelial growth of Pleurotus ostreatus after medium supplementation with various amino acids at different concentrations to select the optimal medium nutrient composition for mycelial growth. The mycelial growth of P. ostreatus was investigated after adding four amino acids (tryptophan, threonine, methionine, and lysine) at 0.5% or 1% to the medium.The rate of P. ostreatus mycelial growthwas faster in the potato dextrose agar (PDA) medium supplemented with threonine at 0.5% or 1% than that of the control, whereas it was inhibited by tryptophan treatment. Supplementation of sawdust mediumwith all amino acids, except tryptophan, at 0.5% did not alter the mycelial growth, compared to the controls. However, addition of any amino acid to sawdust medium at a higher concentration (1%) inhibited the mycelial growth. The laccase acitivity of P. ostreatus mycelium cultured in PDA medium was the highest when threonine was added, and the lowest when tryptophane was added, consistent with the results of the mycelial growth. Therefore, the addition of threonine, methionine, or lysine to PDA medium at a concentration of 0.5-1%was effective for increasing the mycelial growth of P. ostreatus; however, it inhibited mycelial growth insawdust medium, suggesting that the effects of amino acids dependedon the medium nutrient composition.

서 론
재료 및 방법
    공시균주
    Agar 배지 균사체 배양
    톱밥 배지 균사체 배양
    Laccase 활성 조사
    통계 분석
결과 및 고찰
    균사체 생장 조사
    톱밥 배지 균사체 생장량 조사
    Laccase 활성 조사
적 요

• 최성주(공주대학교 식물자원학과) | Seong-Ju Choi (Department of plant Resources, Kongju National University, Yesan 340-702, Korea)
• 박태민(공주대학교 식물자원학과) | Tae-Min Park (Department of plant Resources, Kongju National University, Yesan 340-702, Korea)
• 박윤진(두과녹비자원연구센터) | Youn-Jin Park (Kongju National University Legumes Green Manure Resource Center)
• 오태석(공주대학교 식물자원학과) | Tae-Seok Oh (Department of plant Resources, Kongju National University, Yesan 340-702, Korea)
• 조영구(공주대학교 식물자원학과) | Young-Koo Cho (Department of plant Resources, Kongju National University, Yesan 340-702, Korea)
• 장명준(공주대학교 식물자원학과) | Myoung-Jun Jang (Department of plant Resources, Kongju National University, Yesan 340-702, Korea) Corresponding author